Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing ovarian cancer target ferroferric oxide nano-particle at room temperature

A technology of ferric tetroxide and ovarian cancer, applied in the direction of iron oxide/iron hydroxide, nanotechnology, nanotechnology, etc., can solve the problems of inability to obtain biologically active surface components and structures, destruction of polypeptide biological activity, harsh conditions, etc. To achieve the effect of environmental friendliness of products and improve biocompatibility

Inactive Publication Date: 2014-03-26
TAIYUAN UNIV OF TECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these methods require specific synthesis conditions: high temperature, high pressure, strong oxidants, and complex templates, etc., so the cost of preparing ferric oxide nanoparticles is high, the conditions are harsh, and the nanoparticles cannot obtain biological activity under high temperature and other conditions. Surface composition and structure, in addition, in the process of surface modification (grafting targeting polypeptide) such as the above conditions will cause serious damage to the biological activity of the polypeptide, so the ferric oxide nanoparticles prepared cannot be used in the biomedical field. wide application
The commonly used means of surface modification of Fe3O4 nanoparticles is chemical grafting. If biologically active substances (folate, polypeptide, protein, etc.) Affect the biological activity of biomolecules, and in addition, the particle size of nanoparticles will inevitably change during the grafting process; therefore, a low-temperature, convenient, environmentally friendly, biocompatible, and tumor-targeted Fe3O4 nanoparticles were developed The preparation method is very necessary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing ovarian cancer target ferroferric oxide nano-particle at room temperature
  • Method for preparing ovarian cancer target ferroferric oxide nano-particle at room temperature
  • Method for preparing ovarian cancer target ferroferric oxide nano-particle at room temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dissolve 5.3g ferric chloride hexahydrate and 1.98g ferrous chloride tetrahydrate in 25ml hydrochloric acid solution, stir and mix with a magnetic stirrer for 15 minutes, then add 5mg polypeptide solution to the above solution, continue stirring for 30 minutes, and The dispersion was put into a stoppered Erlenmeyer flask and left at room temperature for 24 hours. Disperse 15g of sodium hydroxide in 250mL of secondary water, stir and mix evenly. At a temperature of 25° C. and a stirring speed of 300 rpm, 250 mL of the above sodium hydroxide aqueous solution was added dropwise to the iron salt and polypeptide solution. Continue stirring at a speed of 300 rpm for 2-6 hours to facilitate the full reaction of the solution. Afterwards, the mixed system was left to stand at room temperature for 2 days to facilitate the nucleation and growth of ferric oxide nanoparticles. The final product is obtained by washing and centrifugation. During the centrifugation process, the reac...

Embodiment 2

[0021] Dissolve 5mg of polypeptide, 5.3g of ferric chloride hexahydrate and 1.98g of ferrous chloride tetrahydrate in 25ml of hydrochloric acid solution, stir and mix with a magnetic stirrer for 15 minutes; disperse 15g of sodium hydroxide in 250mL of secondary water, Stir to combine well. Add the above iron salt solution and lye into the conical flask dropwise at room temperature, and continue stirring at a speed of 300 rpm for 2-6 hours, so as to facilitate the full reaction of the solution. Subsequent washing and centrifugation processes were the same as in Example 1.

Embodiment 3W

[0022] Cytotoxicity research of embodiment 3WSG-Fe3O4 particles

[0023] Cells were seeded in 96-well plates, cultured to 80% coverage, and the medium was aspirated. Add different concentrations of polypeptide-Fe3O4 particle suspension diluted with complete medium, 200μL / well, and culture for 24h, 48h, 72h. 4-6 parallel experiments in each experimental group, 2-3 repeated experiments, with polypeptide-Fe 3 o 4 The particle concentration was 0 group as a control. Add MTT solution at the detection time point, 20 μL / well (keep out of light), and incubate in a 37°C incubator for 24h. Aspirate the liquid in each well, add 150 μL / well dimethyl sulfoxide (DMSO), and incubate at 37° C. for 30 min. The absorbance was measured with a microplate reader at 490 nm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing an ovarian cancer target ferroferric oxide nano-particle at the room temperature. The method comprises the steps of uniformly dispersing ferric salt, ferrous salt and polypeptide into a hydrochloric acid solution, dispersing alkali in water, mixing the two solutions at the room temperature, and preparing the ferroferric oxide nano-particle with good dispersion, diameter distribution of 20-50nm, good biocompatibility and target ovarian cancer cells (SK-OV-3). The method has the environment-friendly characteristics of simplicity and easiness in operation and the like.

Description

technical field [0001] The invention relates to a method for assisting the preparation of ovarian cancer-targeting nano-iron ferric oxide particles by using synthetic polypeptides at room temperature. Background technique [0002] Due to the large surface area, superparamagnetism, and other good physical, chemical, and biological properties of iron ferric oxide nanoparticles, they are widely used in magnetic resonance imaging (MRI), targeted drug delivery, cell separation and screening, and tumor therapy (thermotherapy). therapy) and other fields have great application potential. However, some recent studies have reported that ferric iron tetroxide nanoparticles can reduce the viability, morphological changes, and cytotoxicity of cells such as human macrophages, fibroblasts, epithelial cell lines, smooth muscle cells, umbilical vein endothelial cells, and cancer cells. Larger, and its poor stability (easy to be oxidized), non-targeting and other shortcomings limit the clini...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C01G49/08B82Y30/00B82Y40/00A61K41/00A61K49/14
Inventor 魏延黄棣胡银春王晓君韩志军
Owner TAIYUAN UNIV OF TECH