Remediation complex enzyme preparation for degrading soil PAH pollutant and soil remediation method by using the same

Abstract

The invention discloses a remediation complex enzyme preparation for degrading soil PAH pollutants and a soil remediation method by using the same. The complex enzyme preparation is prepared by the following process: uniformly stirring for mixing 50-60% (V / V) of a laccase crude product enzyme liquid, 20-30% (V / V) of a manganese peroxidase crude product enzyme liquid, 10-20% (V / V) of a lignin peroxidase crude product enzyme liquid and 5-10% (W / V) of soluble starch, carrying out spray drying, controlling the moisture content of a finished product at 3-6%, and conducting aseptic packaging. The invention has the characteristics of simple processing, short effectiveness period and distinct effect; through the synergistic effects of three enzymes, the complex enzyme preparation can degrade the PAH pollutants in the soil; after testing, the complex enzyme preparation has degradation rate on PAH pollutants in the soil as high as 70-80% and can realize efficient detoxification and remediation of contaminated soil.

Description

technical field [0001] The invention belongs to the field of soil remediation, and in particular relates to a compound enzyme preparation capable of degrading soil PAH pollutants and a method for remediating soil with the same. Background technique [0002] Soil is the four elements that make up the environment and plays an irreplaceable role in the natural ecosystem. With the development of industrialization, urbanization and highly intensified agriculture, the pollution caused by industrial waste, agricultural fertilizers, pesticides, and heavy metals / chemicals has become more and more serious, resulting in deteriorating soil quality and seriously affecting ecological security and human health. Soil pollutants mainly include inorganic pollutants and organic pollutants. Inorganic pollutants include heavy metal pollution and radioactive element pollution. Organic pollutants include organic pesticide pollution, herbicide pollution, polycyclic aromatic hydrocarbon pollution (P...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the existing soil PAH pollutant degradation technology, the applicant provided a repairing compound enzyme preparation for degrading soil PAH pollutants through research and improvement. The repairing compound enzyme preparation was prepared by the following method: adding laccase Crude enzyme solution 50-60% (V / V), manganese peroxidase crude product enzyme solution 20-30% (V / V), lignin peroxidase crude product enzyme solution 10-20% (V / V) V) and 5-10% (W / V) of soluble starch, stir and mix evenly, spray dry, control the water content of the finished product to 3-6%, and aseptically package it to obtain; the enzyme activity of the crude laccase product is ≥10 10,000 U / L, the enzyme activity of the crude product of manganese peroxidase is ≥100,000 U / L, the enzyme activity of the crude product of lignin peroxidase is ≥3000 U / L, and the soluble starch is AR class