Remediation complex enzyme preparation for degrading soil PAH pollutant and soil remediation method by using the same

A compound enzyme preparation and pollutant technology, which is applied in the field of soil remediation to achieve the effects of simple treatment, remarkable effect and short effective period

Active Publication Date: 2015-04-29
核工业井巷建设集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the existing soil PAH pollutant degradation technology, the applicant provided a repairing compound enzyme preparation for degrading soil PAH pollutants through research and improvement. The repairing compound enzyme preparation was prepared by the following method: adding laccase Crude enzyme solution 50-60% (V / V), manganese peroxidase crude product enzyme solution 20-30% (V / V), lignin peroxidase crude product enzyme solution 10-20% (V / V) V) and 5-10% (W / V) of soluble starch, stir and mix evenly, spray dry, control the water content of the finished product to 3-6%, and aseptically package it to obtain; the enzyme activity of the crude laccase product is ≥10 10,000 U / L, the enzyme activity of the crude product of manganese peroxidase is ≥100,000 U / L, the enzyme activity of the crude product of lignin peroxidase is ≥3000 U / L, and the soluble starch is AR class

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~ Embodiment 3

[0018] The method for preparing the crude product enzyme liquid used in the following examples 1 to 3 is as follows:

[0019] Enzyme solution of crude laccase product: Pycnoporus sp.SYBC-L3 purchased from CCTCC (preservation number: CCTCC NO: M2010235) was cultured on a slant, the strain was activated, primary seed cultivation, secondary seed cultivation ( Seed medium (w / v): glucose 5%, yeast extract 1%, peptone 0.5%) and fermenter stirring fermentation (fermentation medium (g / L): maltose 12, soybean meal 6, CuSO 4 ·5H 2 00.4, KH 2 PO 4 1, Na 2 HPO 4 0.2, MgSO4 7H 2 O0.5, MnSO 4 ·H 2 O0.034), the fermentation broth was vacuum filtered, and the filtrate was concentrated by ultrafiltration to obtain a concentrated laccase crude product enzyme solution. The enzyme activity of laccase was detected by DMP method to reach more than 100,000 U / L.

[0020] Manganese peroxidase crude product enzyme solution: Phanerochaete chrysosporium (preservation number: CGMCC NO: 5.0776) pur...

Embodiment 1

[0024] Embodiment 1 repair compound enzyme preparation 1

[0025] preparation:

[0026] (1) Liquefaction and dissolution: weigh 60% (V / V) of laccase crude product enzyme solution, 30% (V / V) of manganese peroxidase crude product enzyme solution, and 10% of lignin peroxidase crude product enzyme solution. % (V / V) and 5% (W / V) of soluble starch, and put them into a closed constant temperature stirrer, the stirring speed is 100rpm, the stirring time is 60min, the temperature is 30°C, until the solid is completely dissolved.

[0027] (2) Spray drying: The compound enzyme liquid obtained in step (1) is spray-dried through a spray dryer, and the main parameters are set as follows: inlet temperature 180°C, outlet temperature 65°C, atomizer frequency 50Hz, feed liquid pump frequency 5.5Hz , control the water content of the finished enzyme powder after drying to 3%.

[0028] (3) Aseptic packaging: send the dried enzyme powder prepared in step (2) into an automatic aseptic packaging ma...

Embodiment 2

[0033] Embodiment 2 Repair compound enzyme preparation 2

[0034] preparation:

[0035] (1) Liquefaction and dissolution: Weigh 55% (V / V) of crude laccase enzyme solution, 30% (V / V) of manganese peroxidase crude product enzyme solution, and 15% of lignin peroxidase crude product enzyme solution. % (V / V) and 10% (W / V) of soluble starch, and put them into a closed constant temperature stirrer, the stirring speed is 100rpm, the stirring time is 45min, the temperature is 25°C, until the solid is completely dissolved.

[0036] (2) Spray drying: The compound enzyme solution obtained in step (1) is spray-dried through a spray dryer, and the main parameters are set as follows: inlet temperature 170°C, outlet temperature 60°C, atomizer frequency 40Hz, feed liquid pump frequency 5Hz, The water content of the enzyme powder finished product is controlled to be 4% after drying.

[0037] (3) Aseptic packaging: send the dried enzyme powder prepared in step (2) into an automatic aseptic pac...

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PUM

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Abstract

The invention discloses a remediation complex enzyme preparation for degrading soil PAH pollutants and a soil remediation method by using the same. The complex enzyme preparation is prepared by the following process: uniformly stirring for mixing 50-60% (V / V) of a laccase crude product enzyme liquid, 20-30% (V / V) of a manganese peroxidase crude product enzyme liquid, 10-20% (V / V) of a lignin peroxidase crude product enzyme liquid and 5-10% (W / V) of soluble starch, carrying out spray drying, controlling the moisture content of a finished product at 3-6%, and conducting aseptic packaging. The invention has the characteristics of simple processing, short effectiveness period and distinct effect; through the synergistic effects of three enzymes, the complex enzyme preparation can degrade the PAH pollutants in the soil; after testing, the complex enzyme preparation has degradation rate on PAH pollutants in the soil as high as 70-80% and can realize efficient detoxification and remediation of contaminated soil.

Description

technical field [0001] The invention belongs to the field of soil remediation, and in particular relates to a compound enzyme preparation capable of degrading soil PAH pollutants and a method for remediating soil with the same. Background technique [0002] Soil is the four elements that make up the environment and plays an irreplaceable role in the natural ecosystem. With the development of industrialization, urbanization and highly intensified agriculture, the pollution caused by industrial waste, agricultural fertilizers, pesticides, and heavy metals / chemicals has become more and more serious, resulting in deteriorating soil quality and seriously affecting ecological security and human health. Soil pollutants mainly include inorganic pollutants and organic pollutants. Inorganic pollutants include heavy metal pollution and radioactive element pollution. Organic pollutants include organic pesticide pollution, herbicide pollution, polycyclic aromatic hydrocarbon pollution (P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
Inventor 赵晓联黄庆国周智慧廖祥儒王捷毛飞君
Owner 核工业井巷建设集团有限公司
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