Novel candida mycoderma bacteria RNA (ribonucleic acid) extraction reagent and use method thereof

Abstract

The invention provides a novel candida mycoderma bacteria RNA (ribonucleic acid) extraction reagent and a use method thereof. The reagent is a first special reagent for candida mycoderma bacteria RNA extraction, a lywallzyme is adopted as a wall-breaking reagent, and a complete set of optimized extraction process is provided; the lywallzyme is selected and used for wall breaking of candida mycoderma bacteria, and an optimal wall-breaking time and temperature combination is determined, so that perfect wall breaking of the candida mycoderma bacteria is fully achieved and impurities such as proteins and the like can be removed to the maximum extent; the reagent has the characteristics of high RNA extraction purity and high yield and is high in reliability; extracted RNA can be directly used for subsequent molecular biology experiments, and a better effect is achieved.

Description

technical field [0001] The invention relates to the technical field of medical molecular biology, in particular to a novel Candida RNA extraction reagent and a use method thereof. Background technique [0002] Candida Albicans, also known as Candida, can invade the skin, mucous membranes and internal organs, manifested as acute, subacute or chronic inflammation, and most of them are secondary infections. There are many types of Candida, but only a few can cause disease to humans. Candida albicans (C.albicans) is the most common and most pathogenic, followed by Candida tropicalis (C.tropicalis), others include Candida Krusei (C.krusei), Candida parapsilosis (C.parapsilokis) and Candida pseudotropicalis (C.pseudotropicalis). [0003] The main feature of Candida is that the cells are spherical, oval, cylindrical, elongated, and sometimes irregular; they reproduce by germination, can form candida, and a few form chlamydospores and fungal filaments. Candida is a yeast-type fung...

AI Technical Summary

Problems solved by technology

The hot-acid phenol method takes a long time, uses a lot of reagents, and the phenolic substances added in the experiment are not easy to remove, and eventually it is easy to form brown complexes with RNA, resulting in the complete loss of biological activity of RNA

Although the commonly used wall breaking methods such as nitrogen grinding method and repeated freezing and thawing method are effective, the operation steps are cumbersome and are not suitable for the extraction of RNA from a large number of samples.

The closest technical solution to the proposal of this application is the Trizol method, which has a great influence on the experimental results. The Trizol method often cannot fully destroy the Candida cell wall before extracting Candida albicans RNA, making the extracted RNA very impure. There are remaining proteins, inorganic salt ions and a large amount of organic impurities, etc., which require a lot of follow-up work before they can be applied, so high-quality total RNA cannot be obtained, wasting a lot of manpower and material resources

Images