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A kind of preparation method of adsorbent for adsorbing and degrading water pollutants

A water pollutant, adsorption and degradation technology, applied in the direction of adsorption water/sewage treatment, chemical instruments and methods, other chemical processes, etc., can solve the problems of long cycle of bioremediation technology

Active Publication Date: 2016-03-30
海南椰国热带水果食品加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cycle of bioremediation technology is long, and at the same time, it is limited by whether there are efficient petroleum hydrocarbon degrading bacteria. The physical adsorption method faces the problem of reprocessing the adsorbent after the adsorption is saturated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of an adsorbent for adsorbing and degrading seawater pollutants, comprising the following steps:

[0021] (1) Activate the strain Acetobacter xylinum for fermentation production of biocellulose, and prepare seed liquid for fermentation production: the seed liquid medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Load 500ml of liquid medium in the liter shaker flask, cultivate at 180 rpm and 30 degrees for 24h to obtain seed liquid;

[0022] (2) The seed liquid is added to the fermentation medium at an inoculum of 10% by volume, and the fermentation medium contains: acetic acid: 0.2%, sucrose: 10%, and the rest are coconut water; then insert into the fermentation medium The oil-degrading bacteria are mixed bacteria of Pseudomonas 2, Arthrobacter petrophaga 2 and Phanerochaete chrysosporium 3, and the number of effective bacteria in the fermentation medium after inoculation is 10 8 cfu / mL;

[0023] (3) 14 days of static cultur...

Embodiment 2

[0026] A preparation method of an adsorbent for adsorbing and degrading seawater pollutants, comprising the following steps:

[0027] (1) Activate the strain Acetobacter xylinum for fermentation production of biocellulose, and prepare seed liquid for fermentation production: the seed liquid medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Load 500ml of liquid medium in the liter shaker flask, cultivate at 180 rpm and 30 degrees for 24h to obtain seed liquid;

[0028] (2) adding the seed liquid to the fermentation medium by the inoculum size of 8% by volume, the fermentation medium contains acetic acid: 0.2%, glucose: 10%, and the rest are coconut water; insert petroleum into the fermentation medium again The degrading bacteria are mixed bacteria of Corynebacterium 1, Bacillus methanogenum 3, Pseudomonas pilosula 1, Boletus pineta 1, and Mycelia 1, and the number of effective bacteria in the fermentation medium after inoculation is 10 6 cfu / mL; then add...

Embodiment 3

[0032] A preparation method of an adsorbent for adsorbing and degrading seawater pollutants, comprising the following steps:

[0033] (1) Activate the strain Acetobacter xylinum for fermentation production of biocellulose, and prepare seed liquid for fermentation production: the seed liquid medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Load 500ml of liquid medium in the liter shaker flask, cultivate at 180 rpm and 30 degrees for 24h to obtain seed liquid;

[0034] (2) The seed liquid is added to the fermentation medium at an inoculum of 12% by volume, and the fermentation medium contains acetic acid: 0.2%, sucrose: 5%, fructose: 5%, and the rest are coconut water; The oil-degrading bacteria are inserted into the base, which are Pseudomonas 1, Corynebacterium 1, Bacillus methanogens 1, Arthrobacter petrophaga 1, Nocardia 1 and Umbrella bursae 1, Lactobacillus with bad taste 1, Mixed bacteria of Boletus pinetae 1, Mycelia 1, Clostridium cyanobacteria ...

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Abstract

The invention relates to a method for preparing an adsorbent for absorbing and degrading seawater pollutants, which comprises the following steps: (1) activating the bacterial strain for fermentation production of biological cellulose, and preparing seed liquid for fermentation production; (2) by volume ratio of 8- 12% of the inoculum amount is added to the fermentation medium in which the oil-degrading bacteria are added; (3) standing and fermenting at 25-35°C for 8-20 days; (4) collecting and culturing The biocellulose film produced on the base surface is washed with alkali and dried until the water content is less than 50%. The adsorbent prepared according to this method uses the three-dimensional structure of biocellulose to immobilize petroleum-degrading bacteria in it to form a membrane structure, which can be directly laid on the surface of the water body to increase the contact area between the adsorbent and the surface of the water body; at the same time, the properties of biocellulose Stable, the oil-degrading bacteria in it can be released slowly and efficiently, absorbing and degrading oil pollution in water bodies, and has the characteristics of immobilization, high efficiency, and long-term effectiveness.

Description

technical field [0001] The invention relates to a method for degrading water body pollutants, in particular to a method for preparing an adsorbent for adsorbing and degrading water body pollutants. Background technique [0002] In recent years, with the increasing demand for oil and its products in various countries around the world, oil spill accidents have occurred frequently in the process of offshore mining, loading and unloading, transportation and utilization of oil, which not only caused a large amount of crude oil loss, but also caused serious pollution to the environment. It is increasingly threatening the ecological environment of water bodies and land areas, and has long-term harm to water body environment, natural resources and aquaculture resources. Therefore, oil spill pollution in water bodies has become a major environmental problem that people must face. [0003] At present, there are mainly physical methods, chemical methods and biological methods to deal w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30C02F1/28
Inventor 钟春燕钟宇光
Owner 海南椰国热带水果食品加工有限公司
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