Preparation method of phospholipid biomimic membrane containing aquaporins

An aquaporin and biomimetic membrane technology, applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., can solve the problem of not being able to improve water flux and retention rate at the same time, and achieve the effect of broad application prospects.

Active Publication Date: 2014-04-16
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A new composite membrane containing aquaporin overcomes the shortcomings of traditional separation membranes that cannot simultaneously improve water flux and retention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Mix 2 ml of 1,2-dioleoylphosphatidylcholine at a concentration of 1 mg / ml with 2 ml of a chloroform solution of 1,2-dioleyloxy-3-trimethylaminopropane at a concentration of 1 mg / ml, and wash with Blow dry with nitrogen and vacuum dry, then add 30 ml of PBS buffer solution with pH=7.5, shake and sonicate to prepare mixed phospholipid vesicle solution of two phospholipids; add aquaporin 1 (0.008mg / ml / ) / glycerol (0.1mg / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml) PBS buffer solution 5ml, put the mixed solution into a dialysis bag with a molecular weight cut-off of 7KDa, and then buffer in PBS Dialyze in the solution for 3 days to prepare proteoliposomes with a ratio of protein to phospholipid of 1:50; immerse the polyacrylonitrile membrane prepared by the phase inversion method in 1.5M NaOH solution for 1.5h, wash it with deionized water, and remove the membrane Alternately soak in polyethyleneimine solution and sodium polystyrene sulfonate solution to obtain a composite me...

Embodiment 2

[0026] Mix 2 ml of 1,2-dioleoylphosphatidylcholine at a concentration of 1 mg / ml with 2 ml of a chloroform solution of 1,2-dioleyloxy-3-trimethylaminopropane at a concentration of 1 mg / ml, and wash with Blow dry with nitrogen and vacuum dry, then add 30 ml of PBS buffer solution with pH=7.5, shake and sonicate to prepare the mixed phospholipid vesicle solution of the above two phospholipids; add aquaporin 1 (0.008mg / ml / ) / Glycerol (0.1mg / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml) PBS buffer solution 2.5ml, put the mixed solution into a dialysis bag with a molecular weight cut-off of 7KDa, and then in Dialyze in PBS buffer solution for 3 days to prepare proteoliposomes with a ratio of protein to phospholipid of 1:100; immerse the polyacrylonitrile membrane prepared by the phase inversion method in 1.5M NaOH solution for 1.5h, wash it with deionized water, Soak the membrane alternately in polyethyleneimine solution and sodium polystyrene sulfonate solution to obtain a layer of ...

Embodiment 3

[0028]Mix 2 ml of 1,2-dioleoylphosphatidylcholine at a concentration of 1 mg / ml with 2 ml of a chloroform solution of 1,2-dioleyloxy-3-trimethylaminopropane at a concentration of 1 mg / ml, and use Blow dry with nitrogen and vacuum dry, then add 30 ml of PBS buffer solution with pH=7.5, shake and sonicate to prepare the mixed phospholipid vesicle solution of the above two phospholipids; add aquaporin 1 (0.008mg / ml / ) / Glycerol (0.1mg / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml) PBS buffer solution 1.25ml, put the mixed solution into a dialysis bag with a molecular weight cut-off of 7KDa, and then in Dialyze in PBS buffer solution for 3 days to prepare proteoliposomes with a ratio of protein to phospholipid of 1:200; immerse the polyacrylonitrile membrane prepared by the phase inversion method in 1.5M NaOH solution for 1.5h, wash it with deionized water, Soak the membrane alternately in polyethyleneimine solution and sodium polystyrene sulfonate solution to obtain a layer of assemb...

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Abstract

The invention relates to a preparation method of a phospholipid biomimic membrane containing aquaporins. A composite membrane the surface of which is electronegative is prepared by layer-by-layer assembly, and a phospholipid vesicle solution with electropositivity, which is wrapped with aquaporins, spreads on a composite membrane by an electrostatic bonding effect so as to form a phospholipid dual-layer membrane. According to the invention, the phospholipid biomimic membrane containing aquaporins is prepared by layer-by-layer assembly, after aquaporins is added, the flux of the membrane is improved by 4-5 times compared with the original membrane, and is about 16-28L/m2h (the water flux of the original membrane is 3-5L/m2h); the reject rate of monovalence Na<+1> is improved by more than 40%; the reject rate of bivalence Mg<+2> is improved by more than 65%; and the preparation method of the phospholipid biomimic membrane containing aquaporins can be applied to different fields of water softening, wastewater treatment and the like, and has a wide application prospect.

Description

technical field [0001] The invention relates to a method for preparing a phospholipid biomimetic membrane containing aquaporin, and belongs to the technical field of biomimetic membrane preparation. Background technique [0002] Seawater desalination is one of the ways to solve the shortage of water resources in the world today. In recent years, membrane separation technology has developed rapidly in the field of water treatment by virtue of its advantages of energy saving, high efficiency and environmental protection. The key to membrane water treatment is the performance of the separation membrane. Developing new composite membrane materials to reduce energy consumption and realize efficient and portable water purification technology is a research hotspot and development trend in the field of membrane materials. Aquaporin is an ideal channel for water molecules, which has super water permeability and single selective passage. The new composite membrane containing aquapo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D67/00B01D69/12C02F1/44
Inventor 王志宁王淼琪王莅宋向菊丁万德
Owner OCEAN UNIV OF CHINA
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