Method for separating and enriching chlorogenic acid in tobacco

A technology for separation and enrichment of chlorogenic acid, applied in chemical instruments and methods, preparation of carboxylic acid esters, preparation of organic compounds, etc., can solve problems such as application difficulties, achieve good separation effect, short phase separation time, enrichment Multiplier effect

Active Publication Date: 2015-03-11
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a result of this, the target itself has changed, making further application difficult

Method used

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  • Method for separating and enriching chlorogenic acid in tobacco
  • Method for separating and enriching chlorogenic acid in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The invention is used to extract, separate and enrich the chlorogenic acid in tobacco leaves.

[0027] Take 10g of tobacco leaves crushed to 40 mesh, add 150mL of water, adjust the pH value to 3 with hydrochloric acid, and extract at 50°C for 3 hours; separate the tobacco extract with a conventional centrifuge, put 110mL of the extract into a self-made flotation cell, Add 10 mL of n-octanol and 1 mL of pyrogallol methanol solution as a mixed extraction solvent, pass through nitrogen flotation at a flow rate of 50 mL / min, and perform flotation for 40 minutes. The extractant from the upper layer is the concentrated solution containing chlorogenic acid. After HPLC detection, the calculated content of chlorogenic acid in the tobacco leaves is 1.55%.

Embodiment 2

[0029] The invention is used to extract, separate and enrich the chlorogenic acid in the tobacco dust.

[0030] Take 10g of tobacco powder, add 200mL of water, adjust the pH value to 5 with hydrochloric acid, extract at 60°C for 4 hours; separate the tobacco extract with a conventional centrifuge, put 150mL of the extract into a self-made flotation cell, add n-hexanol 2.5mL and 0.5mL of acetonitrile mixed extraction solvent, pass nitrogen flotation at a flow rate of 90mL / min, flotation for 20min, after the end, stand still for 20min, until there are no microbubbles in the flotation cell, separate the upper layer extractant, which is The concentrated solution containing chlorogenic acid is detected by HPLC, and the calculated content of chlorogenic acid in the tobacco powder is 1.96%.

Embodiment 3

[0032] The invention is used to extract, separate and enrich the chlorogenic acid in tobacco stems.

[0033] Take 10g of tobacco stems crushed to 80 mesh, add 250mL of water, adjust the pH value to 4 with hydrochloric acid, extract at 70°C for 2 hours; separate the tobacco extract with a conventional centrifuge, and put 210mL of the extract into a self-made flotation tank , add a mixed extraction solvent of 5 mL of n-decyl alcohol and 2 mL of pyrogallol methanol solution, pass nitrogen flotation at a flow rate of 70 mL / min, and carry out flotation for 30 minutes. The extractant in the upper layer was separated, which was the concentrated solution containing chlorogenic acid, and the content of chlorogenic acid in the tobacco stem was calculated to be 1.26% through HPLC detection.

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Abstract

The invention relates to a method for separating and enriching chlorogenic acid in tobacco. According to the method, the chlorogenic acid in tobacco is separated and enriched by utilizing a solvent flotation technology, and a mixed organic solvent is added to serve as an extracting agent, so that the polarity of the extracting agent is changed, and the extraction rate of the chlorogenic acid is improved. The pH value of a tobacco extracting solution is regulated, so that the polarity of the extracting agent is matched with that of the chlorogenic acid, and the separation, enrichment and purification effect is good. The extraction can be performed at room temperature due to the adopted solvent flotation technology, and the operation conditions are mild. According to floatation of an air floatation solvent, an emulsification phenomenon generated due to shaking during common liquid-liquid extraction is avoided, the amount of the used organic solvent is small, and the method is large in enrichment factor, short in phase separation time, low in cost and feasible to operate.

Description

technical field [0001] The invention relates to the technical field of separation of chlorogenic acid in tobacco, in particular to a method for separating and enriching chlorogenic acid in tobacco. Background technique [0002] my country is a big tobacco leaf planting country, and there is a large amount of tobacco leaves and cigarette leftovers urgently waiting to be used every year. Tobacco leaves contain a variety of valuable chemical components, among which chlorogenic acid is one of the most important phenolic substances in tobacco leaves, accounting for about 75% to 90% of the total polyphenols. Chlorogenic acid, also known as coffee tannin, is a phenylpropanoid compound produced by plants through the shikimic acid pathway during aerobic respiration. It contains 3% or higher chlorogenic acid in tobacco leaves. The highest content of polyphenolic compounds and the only tannins found in tobacco. It has an important impact on the growth and development of tobacco, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/58
CPCC07C67/58C07C69/732
Inventor 李小兰孟冬玲黄善松周艳玫范忠蒋光辉王维刚
Owner CHINA TOBACCO GUANGXI IND
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