Polydopa/polyethyleneimine copolymer modified magnetic particles and preparation method and application thereof

A polyethyleneimine, polydopa technology, applied in chemical/physical process, preparation of test samples, alkali metal oxides/hydroxides, etc., can solve unfavorable catecholamine adsorption, polydopa coating preparation time long, limited number of amino groups, etc., to achieve the effects of good dispersion, mild conditions and easy operation

Active Publication Date: 2019-06-28
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation time of the polydopa coating in this patent is too long, exceeding 8 hours, and the thickness of the coating is only 3nm. At the same time, the number of amino groups in the coating is limited, which is not conducive to its adsorption of catecholamines.

Method used

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  • Polydopa/polyethyleneimine copolymer modified magnetic particles and preparation method and application thereof
  • Polydopa/polyethyleneimine copolymer modified magnetic particles and preparation method and application thereof
  • Polydopa/polyethyleneimine copolymer modified magnetic particles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of polyDOPA / PEI-MNPs includes the following steps:

[0039] (1) Preparation of COOH-MNPs: 4mmol / L FeCl 3 ·6H 2 O was added to 30mL of diethylene glycol and 10mL of ethylene glycol for ultrasonic-assisted dissolution, then 3g of anhydrous sodium acetate and 3g of sodium acrylate were added in sequence, and stirred in a constant temperature water bath at 70°C at a speed of 500r / min for 1h to form a homogeneous solution. Pour it into a Teflon high-pressure reactor, put it in an oven, heat up to 200°C, react for 10 hours, cool to room temperature, separate the product under an external magnetic field and wash it with ethanol and deionized water for 3 times, and dry it at 60°C Uniformly dispersed COOH-MNPs were obtained.

[0040] (2) 160 mg of dopa was dissolved in Tris-HCl aqueous solution (160 mL, 10 mmol / L, pH 8.5) to prepare a dopa solution. Then, 40 mg COOH-MNPs were weighed and dispersed in dopa solution, followed by polyethyleneimine (2 mg / mL), CuSO ...

Embodiment 2

[0048] The preparation of polyDOPA / PEI-MNPs includes the following steps:

[0049] (1) Preparation of COOH-MNPs: 5mmol / L FeCl 3 ·6H 2 O was added to 20mL of diethylene glycol and 20mL of ethylene glycol for ultrasonic-assisted dissolution, and then 6g of anhydrous sodium acetate and 3g of sodium acrylate were added in sequence, and stirred in a constant temperature water bath at 50°C at a speed of 300r / min for 1h to form a homogeneous solution. Pour it into a Teflon autoclave, put it in an oven, raise the temperature to 180°C, react for 15 hours, cool to room temperature, separate the product under an external magnetic field and wash it three times with de-ethanol and deionized water successively, and dry at 60°C Dry to obtain uniformly dispersed COOH-MNPs.

[0050] (2) 80 mg of dopa was dissolved in Tris-HCl aqueous solution (160 mL, 30 mmol / L, pH 7.5) to prepare a dopa solution. Then, 40 mg COOH-MNPs were weighed and dispersed in dopa solution, followed by polyethyleneimi...

Embodiment 3

[0058] The preparation of polyDOPA / PEI-MNPs includes the following steps:

[0059] (1) Preparation of COOH-MNPs: 6mmol / L FeCl 3 ·6H 2 O was added to 32mL of diethylene glycol and 8mL of ethylene glycol for ultrasonic dissolution, then 9g of anhydrous sodium acetate and 3g of sodium acrylate were added in sequence, and stirred in a constant temperature water bath at 60°C at a speed of 400r / min for 1h to form a homogeneous solution. Pour it into a Teflon autoclave, put it in an oven, raise the temperature to 190°C, react for 20 hours, cool to room temperature, separate the product under an external magnetic field and wash it three times with de-ethanol and deionized water successively, and dry at 60°C Dry to obtain uniformly dispersed COOH-MNPs.

[0060] (2) 320 mg of dopa was dissolved in Tris-HCl aqueous solution (160 mL, 50 mmol / L, pH 9.5) to prepare a dopa solution. Then, 40 mg COOH-MNPs were weighed and dispersed in dopa solution, followed by polyethyleneimine (2 mg / mL),...

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Abstract

The invention discloses polydopa/polyethyleneimine copolymer modified magnetic particles and a preparation method and application thereof. The preparation method of the polydopa/polyethyleneimine copolymer modified magnetic particles includes the following steps that COOH-MNPs is added into an alkaline solution of dopa, then polyethyleneimine, CuSO4 and H2O2 are added to serve as inducers, the material is heated to 25-50 DEG C to be stirred for 0.5-2.0 h, and polyDOPA/PEI-MNPs is obtained after separation. The polyDOPA/PEI-MNPs has mild preparation conditions, the operation is easy and convenient, the reproducibility and the stability are good, and separation and enrichment of catecholamine in diluted body fluid (especially blood) can be achieved.

Description

technical field [0001] The invention belongs to the technical field of magnetic solid-phase extraction, in particular to polydopa / polyethyleneimine copolymer modified magnetic particles (polyDOPA / PEI-MNPs) and its preparation and application in the field of biomedicine, especially suitable for catecholamine extraction Analytical applications of sex enantiomers. Background technique [0002] Epinephrine (EP), norepinephrine (NE) and isoproterenol (IP) belong to the catecholamines, which all contain o-dihydroxybenzene nucleus and ethylamine group. The core structure of catecholamines contains a chiral carbon atom, therefore, each catecholamine exists as an enantiomer. [0003] EP and NE are widely used as drugs to treat neurological disorders such as Parkinson's disease. IP is used to treat acute allergic symptoms, bronchial asthma, glaucoma, heart block, cardiac arrest, and bradycardia, among others. These drugs are usually administered as racemates. However, due to the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30B01D15/08G01N1/34G01N1/40G01N21/3563G01N27/00G01N27/447
Inventor 贾丽吴佳
Owner SOUTH CHINA NORMAL UNIVERSITY
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