SNP marker related to Yili horse milk traits and detection method thereof

A detection method and marker technology, applied in the field of animal molecular genetics and breeding, can solve the problem of insignificant differences in milk production

Active Publication Date: 2014-04-23
刘武军 +1
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AI Technical Summary

Problems solved by technology

The study on the correlation between PRL exon 5 and PRLR exon 4 polymorphic genotypes and milk production in three different breeds of horse populations showed that in the Yili horse population, there was a significant difference in milk production between the two genotypes. However, in the new Jima and hybrid horse populations, the milk production difference between individuals of different genotypes was not significant; the mutations in exon 5 of the PRL gene and exon 4 of the PRLR gene may affect the milk production performance of Yili horses important site

Method used

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  • SNP marker related to Yili horse milk traits and detection method thereof
  • SNP marker related to Yili horse milk traits and detection method thereof
  • SNP marker related to Yili horse milk traits and detection method thereof

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Experimental program
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Embodiment

[0026] 1. Extraction of horse genomic DNA

[0027] Using a blood collection tube filled with sodium citrate anticoagulant, collect 5ml of Ili horse jugular venous blood from the slaughterhouse and store it at -20°C;

[0028] According to the "Molecular Cloning Experiment Guide", the genomic DNA was extracted by phenol-chloroform extraction and stored at 4°C.

[0029] 2. Primer design and synthesis

[0030] According to the SNP site of the horse (GenBank accession number: BIEC2-560774), specific primers were designed with reference to foreign literature, and the primers were ordered and synthesized in Shanghai Bioengineering Technology Co., Ltd. The primer sequences are as follows:

[0031] Upstream primer: 5'-GGTTGCTATTCTGGGCTTTG-3'

[0032] Downstream primer: 5'-TGTGTTGGGATGGACAAAAA-3'

[0033] Primer size: 194bp

[0034] Optimum annealing temperature: 57.1°C

[0035] 3. PCR amplification

[0036] 1) PCR amplification reaction system, see Table 1.

[0037]

[0038]...

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Abstract

The invention provides a SNP (single nucleotide polymorphism) marker related to Yili horse milk traits and a detection method thereof. The SNP marker is positioned on a base at a 29764513 site on a horse chromosome 21, and the average milk production amount of a Yili horse having the 29764513 site with a base G is significantly higher than that of a Yili horse having the 29764513 site with a base A. The genotype of a to-be-detected Yili horse is detected by a molecular marker technology. Breeding selection of the Yili horse milk production amount is carried out according to the genotype, and thus breeding of Xinjiang specialized milk horse breeds is accelerated.

Description

technical field [0001] The invention relates to animal molecular genetic breeding, which utilizes DNA molecular marker technology to detect the correlation with milk traits of Yili horses, and selects a method for improving the milk yield of Yili horses. Background technique [0002] Mare's milk is sweet and cool in nature and has the highest nutritional value among all kinds of dairy products. It contains protein, fat, sugar, phosphorus, calcium, potassium, sodium, vitamin A, vitamin B1-B2, vitamin C, niacin, inositol It is rich in vitamins and minerals, easy to be digested and absorbed by the human body, and has the functions of nourishing and strengthening the body, moistening dryness and beautifying the skin, clearing away heat and quenching thirst. Since mare's milk only contains 1.5% fat, it is less than cow's milk. double. The kumiss made from fermented mare's milk is not only cool and delicious, but also rich in nutrition. It can also nourish the spleen and stomach,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘武军邵勇钢王琼莫合塔尔·夏甫开提孟军耿明刘玲玲
Owner 刘武军
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