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A gas-controlled solid-phase polypeptide synthesis microfluidic chip device and its application

A solid-phase peptide synthesis and microfluidic chip technology, which is applied in peptides, peptide preparation methods, chemical/physical/physicochemical processes, etc., can solve problems such as difficult direct application, achieve low cost, and realize the effect of automatic control

Active Publication Date: 2015-09-23
BEIJING NANOPEP BIOTECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, solid-phase peptide synthesis requires the use of strong organic solvents such as dimethylformamide (DMF), dichloromethane (DCM), and trifluoroacetic acid (TFA) and volatile strong acids. Ordinary pumps, valves, etc. are difficult to use directly, and more ingenious designs are needed to ensure automated solid-phase peptide synthesis on microfluidic chips

Method used

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  • A gas-controlled solid-phase polypeptide synthesis microfluidic chip device and its application
  • A gas-controlled solid-phase polypeptide synthesis microfluidic chip device and its application
  • A gas-controlled solid-phase polypeptide synthesis microfluidic chip device and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Example 1, Fabrication of gas-controlled solid-phase polypeptide synthesis microfluidic chip device

[0061] (1) Fabrication of synthetic reaction microfluidic chip

[0062] The production process of the synthetic reaction microfluidic chip includes: exposure, development, chromium removal, glass wet etching, delustering glue, chrome removal and bonding steps, as follows:

[0063]The pattern of the designed synthetic reaction microfluidic chip cover sheet is made into a film mask by a high-resolution printer; the film mask and the homogeneous chrome plate (chrome type: LRC; chrome thickness: 145nm; glue thickness: 570nm ) in a UV lithography machine with an exposure time of 5 seconds; the exposed chromium plate was developed in an aqueous solution of 0.7% by mass of NaOH, and the pattern on the mask was copied to the photoresist layer; Put the developed homogeneous chromium plate into the chromium removal solution (the mass-volume concentration of ammonium cerium nitra...

Embodiment 2

[0072] Example 2, using the device made in Example 1 to carry out solid-phase peptide synthesis

[0073] As the solid phase carrier, commercial Wang resin is selected, and the amino acid at the C-terminal of the polypeptide is connected to the resin in advance according to the sequence of the polypeptide to be synthesized. The polypeptide sequence to be synthesized in this example is Tyr-Gly-Gly-Phe-Leu. In this example, a commercially available Fmoc-Leu modified solid-phase peptide synthesis carrier was used as the solid-phase carrier, and the peptide was synthesized using the air-controlled solid-phase peptide synthesis microfluidic chip device prepared in Example 1.

[0074] Concrete synthetic steps are as follows:

[0075] a. This step is a manual operation step, and the synthetic carrier is loaded. Use a syringe to pour Fmoc-Leu-modified solid-phase peptide synthesis carrier (resin) dispersed in N'N-dimethylformamide (DMF) into the reaction cavity from the main injectio...

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Abstract

The invention relates to a pneumatic control solid-phase polypeptide micro-fluidic chip device and an application thereof. The pneumatic control solid-phase polypeptide micro-fluidic chip device comprises a synthetic reaction micro-fluidic chip, a reagent charging pool array, a plurality of paths of gas electromagnetic valves and a constant-pressure air source, wherein a runner structure is arranged inside the synthetic reaction micro-fluidic chip and comprises a main runner and a plurality of branch runners, a reaction cavity is arranged at a place, which is close to an outlet, of the main runner, one end of each branch runner is communicated with the main runner, the reagent charging pool array comprises a plurality of reagent charging pools, each reagent charging pool is communicated with the synthetic reaction micro-fluidic chip, the plurality of paths of gas electromagnetic valves are provided with common input ends communicated with the constant-pressure air source, each path of gas electromagnetic valve is provided with an output end communicated with each reagent charging pool, and the constant-pressure air source is used for outputting a pressure with a set pressure range to the plurality of gas electromagnetic valves. The pneumatic control solid-phase polypeptide micro-fluidic chip device provided by the invention can be used for synthesis of continuously flowing solid-phase polypeptide.

Description

technical field [0001] The invention relates to the technical field of polypeptide synthesis, in particular to the technical field of solid-phase polypeptide synthesis, in particular to a gas-controlled solid-phase polypeptide synthesis microfluidic chip device and its application. Background technique [0002] Solid-phase chemical synthesis is an important application of heterogeneous reactions, which was founded by Nobel Prize winner Merrifield and has been further developed since then. Solid-phase peptide synthesis is one of the most widely used solid-phase chemical synthesis (J. Am. Chem. Soc., 1963, 85, 2149-2154.). [0003] The solid-phase peptide synthesis method is to connect the reactants to the polymer skeleton with active groups, introduce the reactants through the fluid phase to undergo chemical synthesis reactions with the solids, and remove impurities and by-products, and finally use appropriate chemical The method cleaves the target polypeptide. Solid-phase ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/04B01J19/00
Inventor 郑晖王蔚芝胡志远
Owner BEIJING NANOPEP BIOTECH CORP LTD
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