Bifidobacterium and lactobacillus mixed fermentation medium and fermentation method

Abstract

The invention discloses a bifidobacterium and lactobacillus mixed fermentation medium. The formula of the bifidobacterium and lactobacillus mixed fermentation medium comprises the following ingredients: 0.5-2 g / L of water-soluble fish meal, 1-5 g / L of water-soluble soybean meal, 2-8 g / L of high-nitrogen corn steep liquor dry powder, 30-50 g / L of high-nitrogen syrup yeast powder, 10-20 g / L of glucose, 1-2 g / L of lactose, 0.1-0.3 g / L of sodium acetate, 2-4 g / L of tween-80, 5-20 mg / L of ammonium ferric citrate, 5-15 mg / L of manganese sulfate and 30-50 mg / L of ferric chloride. The medium is used for mixed culturing of the bifidobacterium and the lactobacillus in a jar, and the viable count is as high as (0.6-2.0)*10<10> cfu / ml. The medium is low in cost and simple in preparation. The mixed fermentation method disclosed by the invention adopts an optimized medium and optimized supplementary material medium ingredients and proportion, and a synergistic effect of the bifidobacterium and the lactobacillus is fully played, thus the production cost is lowered and the utilization rate of equipment is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a mixed fermentation medium of bifidobacterium and lactobacillus and a fermentation method thereof. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Beneficial bacteria or fungi in humans and animals mainly include Clostridium butyricum, lactic acid bacteria, bifidobacteria, Lactobacillus acidophilus, actinomycetes, and yeasts. At present, the most powerful products researched in the world are mainly compound active probiotics composed of the above-mentioned various microorganisms, which are widely used in the fields of bioengineering, industry and agriculture, food safety, a...

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Problems solved by technology

There are also a small number of domestic and foreign literatures reporting the mixed fermentation method of bifidobacteria and lactobacillus, but all have their shortcomings, such as high cost, or too low number of viable bacteria, etc.

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