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Chocolate microbacterium magnetic cell as well as preparation method and application thereof

A microbacterium and chocolate technology, applied in the field of microbacteria chocolate magnetic cells and its preparation, can solve the problems of weak binding force between cells and materials, inability to reuse, low stability, etc., and achieve convenient separation, enzyme activity maintenance, and low activity Effect

Inactive Publication Date: 2014-04-30
SHANGHAI INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One of the purposes of the present invention is to provide a kind of microbacterium chocolate in order to solve the above-mentioned technical problems such as weak binding force between cells and materials, low stability, easy to fall off in the batch reaction process of repeated use and unable to be reused. magnetic cell

Method used

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  • Chocolate microbacterium magnetic cell as well as preparation method and application thereof
  • Chocolate microbacterium magnetic cell as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A magnetic microbacterium chocolatier cell is specifically prepared by a method comprising the following steps:

[0042] (1), Chitosan-wrapped Fe 3 o 4 Preparation of Magnetic Nanomaterials

[0043] Dissolve 0.2g of chitosan (viscosity 400-600mPa.s) in 20ml of acetic acid aqueous solution with a mass percent concentration of 5%. After the chitosan is completely dissolved, add Fe 3 o 4 Particles 0.2g, ultrasonically mixed for 20 minutes to obtain a mixture;

[0044] Then, under strong stirring in a water bath at 40°C, disperse the resulting mixed solution in a three-necked flask filled with 40ml of liquid paraffin and 5ml of Span 80, stir for 30min, add 3ml of glutaraldehyde aqueous solution with a concentration of 25% by mass, and mechanically stir After reacting for 4 hours, the chitosan-coated Fe 3 o 4 Crude magnetic nanomaterials;

[0045] The obtained chitosan-coated Fe was fully ultrasonically washed with petroleum ether 3 o 4 The crude magnetic nanomateri...

Embodiment 2

[0057] A microbacterium chocolatier magnetic cell, specifically prepared by the method comprising steps as described in Example 1:

[0058] Just the Microbacterium chocolatier cells used in step (3) and the chitosan-coated Fe 3 o 4 The weight ratio and stirring time of magnetic nanomaterial are different from Example 1, and others are all the same as Example 1;

[0059] The used Microbacterium chocolatier cells with chitosan-coated Fe 3 o 4 The weight ratio of magnetic nanomaterials: that is, the used chitosan-wrapped Fe 3 o 4 Amount of magnetic nanomaterials, Microbacterium chocolate cells, by chitosan-coated Fe 3 o 4 The wet weight of magnetic nanomaterials: the dry weight of Microbacterium chocolate cells: the ratio of phosphate buffer solution is 150g: 10g: 1L;

[0060] Stirring time is 1h.

[0061] Others are the same as embodiment 1.

[0062] The immobilization rate of the magnetic cells of Microbacterium chocolate was calculated by the formula of immobilization...

Embodiment 3

[0064] A microbacterium chocolatier magnetic cell, specifically prepared by the method comprising steps as described in Example 1:

[0065] Just the Microbacterium chocolatier cells used in step (3) and the chitosan-coated Fe 3 o 4 The weight ratio and stirring time of magnetic nanomaterial are different from Example 1, and others are all the same as Example 1;

[0066] The used Microbacterium chocolatier cells with chitosan-coated Fe 3 o 4 The weight ratio of magnetic nanomaterials: that is, the used chitosan-wrapped Fe 3 o 4 Amount of magnetic nanomaterials, Microbacterium chocolate cells, by chitosan-coated Fe 3 o 4 The wet weight of magnetic nanomaterials: the dry weight of Microbacterium chocolate cells: the ratio of phosphate buffer solution is 150g: 10g: 1L;

[0067] Stirring time is 3h.

[0068] Others are the same as embodiment 1.

[0069] The immobilization rate of the magnetic cells of Microbacterium chocolate was calculated by the formula of immobilization...

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Abstract

The invention discloses a chocolate microbacterium magnetic cell as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, preparing a Fe3O4 magnetic nano material wrapped by chitosan, then preparing the cell suspension of a chocolate microbacterium, finally adding the obtained Fe3O4 magnetic nano material wrapped by the chitosan into the cell suspension of the chocolate microbacterium, controlling the temperature to below 30 DEG C, and stirring and adsorbing for 10-600 min, so that the chocolate microbacterium magnetic cell is obtained. The chocolate microbacterium magnetic cell is used for catalyzing asymmetric hydrolysis reaction of meso dimethyl ester to generate (4S and 5 R) semi-ester, and can be recycled. After the chocolate microbacterium magnetic cell is recycled for 10 times, the catalytic conversion rate of the chocolate microbacterium magnetic cell for the substrate biotin dimethyl ester is still more than 70 percent. The chocolate microbacterium magnetic cell has the advantages of simple preparation method, high recovery rate of enzyme activity and good stability, and can be conveniently and quickly recycled and reused in a magnetic field.

Description

technical field [0001] What the present invention relates to is a kind of Microbacterium chocolate magnetic cell and its preparation method and application, more specifically relate to a kind of using chitosan-Fe 3 o 4 Microbacterium chocolate magnetic cells prepared by immobilizing Microbacterium chocolate with magnetic nanoparticles and its ability to catalyze the generation of asymmetric hydrolysis of meso-dimethyl ester (4 S , 5 R )-application in half esters. Background technique [0002] Cell immobilization refers to a basic technology that uses chemical or physical means to locate free cells in a limited space and keep them active and reusable. [0003] Compared with free cells, immobilized cells have significant advantages. For example, immobilized cells can have higher stability, can be reused, and the immobilization method is simpler and lower in cost. Commonly used methods for immobilizing cells include embedding and adsorption. The embedding method is a meth...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12P17/10C12R1/01
CPCY02P20/50
Inventor 徐毅陈建波周音卉郑蒙蒙吴范宏朱勇刚吴小梅徐峰蒲强卞蒙璐周敏何梨梨
Owner SHANGHAI INSTITUTE OF TECHNOLOGY
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