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Method used for removing lignocelluloses enzymatic hydrolysate inhibitors

A technology of lignocellulose and enzymatic hydrolysis solution, applied in sugar production, food science, sugar production, etc., can solve the problems of sugar consumption and high enzyme cost, and achieve adsorption capacity recovery, high selectivity, and high removal rate effect

Active Publication Date: 2015-05-13
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of enzymes is high, and microorganisms need to be cultivated and will consume sugars in the hydrolyzate

Method used

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  • Method used for removing lignocelluloses enzymatic hydrolysate inhibitors

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The steamed material was adjusted to pH 5 with NaOH, added with Shandong Zesheng cellulase, hydrolyzed at 50°C for 120 hours, and centrifuged to obtain the enzymatic hydrolyzate.

[0033] (1) At a flow rate of 3.5mL / min, a pH value of 5, and a temperature of 20°C, the enzymatic hydrolysis solution was put into an adsorption chromatography column of φ30*500mm in an upflow manner, and the resin was NKA-Ⅱ (purchased from Nankai University Chemical Factory ), the filling height of NKA-Ⅱ resin is 300mm, and the absorbance of the outflowing enzymatic hydrolysis solution is detected with a UV spectrophotometer. When the absorbance is less than 0.2, stop entering the enzymatic hydrolysis solution. The adsorption capacity of fresh NKA-II resin was detected by experiments to be 140 mg / g (based on phenol).

[0034] (2) Drain the enzymolysis solution above the chromatography column and return it to the enzymolysis solution tank. The resin layer drops while the liquid is drained, and ...

Embodiment 2

[0041] The steamed material was adjusted to pH 5 with NaOH, added with Shandong Zesheng cellulase, hydrolyzed at 50°C for 120 hours, and centrifuged to obtain the enzymatic hydrolyzate.

[0042] (1) At a flow rate of 5.0mL / min, a pH value of 5, and a temperature of 20°C, the enzymatic hydrolysis solution was poured into a φ30*500mm adsorption chromatography column in an upflow manner, and the resin was NKA-Ⅱ (purchased from Nankai University Chemical Plant) , The filling height of NKA-Ⅱ resin is 300mm, and the absorbance of the outflowing enzymatic hydrolysis solution is detected with a UV spectrophotometer. When the absorbance is less than 0.2, stop entering the enzymatic hydrolysis solution. The adsorption capacity of NKA-II resin was detected by experiments to be 140 mg / g (based on phenol).

[0043] (2) Drain the enzymolysis solution above the chromatography column and return it to the enzymolysis solution tank. The resin layer drops while the liquid is drained, and part of...

Embodiment 3

[0050] The steamed material was adjusted to pH 5 with NaOH, added with Shandong Zesheng cellulase, hydrolyzed at 50°C for 120 hours, and centrifuged to obtain the enzymatic hydrolyzate.

[0051] (1) Enter the enzymatic hydrolysis solution into the adsorption chromatography column of φ30*500mm in the upflow mode at the flow rate of 7mL / min, pH value of 5, and temperature of 20°C. The resin is NKA-Ⅱ (purchased from Nankai University Chemical Plant), The filling height of NKA-Ⅱ resin is 300mm. Use an ultraviolet spectrophotometer to detect the absorbance of the outflowing enzymolysis solution. When the absorbance is less than 0.2, stop entering the enzymolysis solution. The adsorption capacity of fresh NKA-II resin is 140 mg / g (based on phenol) as detected by experiments.

[0052] (2) Drain the enzymolysis solution above the chromatography column and return it to the enzymolysis solution tank. The resin layer drops while the liquid is drained, and part of the saturated resin is d...

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Abstract

The invention discloses a method used for removing lignocelluloses enzymatic hydrolysate inhibitors. According to the method, a lignocelluloses enzymatic hydrolysate is delivered through a polar macroporous resin chromatographic column in an upper current manner; a sulfite is added for further detoxification; H2O2-ozone-UV method is adopted for chemical oxidative degradation of the inhibitors in the saturated resin, and an organic solvent and water are used for resin regeneration; and the regenerated adsorption resin is recycled. Detoxification effects of the method on the lignocelluloses enzymatic hydrolysate are excellent; glucose loss ratio and xylose loss ratio are low; and at the same time, the absorption resin is subjected to oxidative degradation firstly, and then is washed for regeneration, so that absorption performance is recovered preferably, resin regeneration times are reduced, and resin utilization ratio is increased.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for detoxifying enzymatic solution by using polar macroporous adsorption resin and sulfite method, which is mainly used for various plant straws such as corn stalks, wheat stalks and sweet sorghum stalks The detoxification of the enzymatic hydrolyzate or the detoxification of its pretreatment hydrolyzate. Background technique [0002] During the pretreatment process, due to the degradation of sugars and lignin, some inhibitors of microbial growth will be released, including weak acid inhibitors (formic acid, acetic acid, levulinic acid, etc.), furfural inhibitors (furfural, 5-hydroxymethyl furfural) and phenolic inhibitors (vanillin, hydroquinone, 4-hydroxybenzoic acid) and other substances. These inhibitors, especially phenols, have obvious inhibitory effect on subsequent microbial fermentation and reduce product yield, so necessary detoxification treatm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C13K1/04C13K13/00
Inventor 唐开宇
Owner CHINA PETROLEUM & CHEM CORP
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