Rice peptide production method

A production method, rice peptide technology, applied in the field of rice peptide production, can solve the problems of lower product protein purity, low yield of rice peptide, long processing cycle, etc., achieve short production cycle, high product purity, and increase added value Effect

Inactive Publication Date: 2014-05-14
江苏联海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some of the existing enzyme modification technologies introduce other components for graft coupling, which reduces the purity of the product protein, and the process is complicated and the processing cycle is long; some use acid-base treatment in the pre-treatment stage to easily introduce Chemical residues; although some only use simple enzyme modification technology, the yield of rice peptide is not high

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0030] Rice peptide production method, concrete steps are as follows:

[0031] A. Weigh 5000g of 100-mesh rice protein powder and put it into the enzymolysis tank, add 25L of 2% sodium sulfite solution, fully stir the material liquid, control the temperature of the enzymolysis tank at 45°C, and continue to keep warm after the temperature of the enzymolysis tank is stable 30min. A rice protein slurry is obtained.

[0032] B, the pH of rice protein slurry is controlled at 9.0 with 1mol / L sodium hydroxide solution. Add 5g of alkaline protease for enzymolysis. During the enzymolysis process, use 1mol / L sodium hydroxide solution to control the pH of the reaction at 9.0, keep stirring for 2.5h, then stop adding alkali, and continue stirring for 0.5h.

[0033] C, adjust the pH of the rice protein slurry through alkaline protease treatment to 7.2 with 1mol / L hydrochloric acid solution, add neutral protease 25g, compound flavor protease 25g and papain 100g to carry out enzymolysis at...

Embodiment 2

[0038] Rice peptide production method, concrete steps are as follows:

[0039] A. Weigh 5000g of 300-mesh rice protein powder and put it into the enzymolysis tank, add 50L of 1% sodium sulfite solution, fully stir the material liquid, control the temperature of the enzymolysis tank at 65°C, and continue to keep warm after the temperature of the enzymolysis tank is stable 75min. A rice protein slurry is obtained.

[0040] B. After the temperature of the feed liquid is stabilized, the pH of the rice protein slurry is controlled at 8.5 with 1mol / L sodium hydroxide solution. Add 125g of alkaline protease for enzymolysis. During the enzymolysis process, use 1mol / L sodium hydroxide solution to control the pH of the reaction at 8.5, keep stirring for 0.5h, then stop adding alkali, and continue stirring for 2.5h.

[0041] C. Use 1mol / L hydrochloric acid solution to adjust the pH of the rice protein slurry treated with alkaline protease to 7.0. At the same time, add 5g of neutral pro...

Embodiment 3

[0046] Rice peptide production method, concrete steps are as follows:

[0047] A. Weigh 5000g of 600-mesh rice protein powder and put it into the enzymolysis tank, add 75L of 1% sodium sulfite solution, fully stir the material liquid, control the temperature of the enzymolysis tank at 75°C, and continue to keep warm after the temperature of the enzymolysis tank is stable 120min. A rice protein slurry is obtained.

[0048] B. After the temperature of the feed liquid is stabilized, the pH of the rice protein slurry is controlled at 7.5 with 2mol / L sodium hydroxide solution. Add 150g of alkaline protease for enzymolysis. During the enzymolysis process, use 2mol / L sodium hydroxide solution to control the pH of the reaction at 8.5, keep stirring for 1.5h, then stop adding alkali, and continue stirring for 1.5h.

[0049] C. Use 2mol / L hydrochloric acid solution to adjust the pH of the rice protein slurry treated with alkaline protease to 6.5, and add 100g of neutral protease, 100g o...

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Abstract

The invention discloses a rice peptide production method. The rice peptide production method comprises the steps of (A) mixing rice protein and a sodium sulfite solution to obtain rice protein slurry; (B) adding alkaline protease into the rice protein slurry to perform enzymolysis; (C) adding neutral protease, compound flavourzyme and papain for enzymolysis to obtain enzymatic hydrolysate; (D) performing enzyme deactivation treatment on the enzymatic hydrolysate; (E) filtering the enzymatic hydrolysate subjected to the enzyme deactivation and then performing freeze drying to obtain a high-purity rice peptide product. The rice peptide production method utilizes the synergistic effect of multiple enzymes, performs rice protein modification by adopting a multiple-process efficient separation method and is short in production period, high in yield and product purity and good in solubility through enzymolysis technology exploration and control.

Description

technical field [0001] The invention relates to the technical field of rice protein processing, in particular to a production method of rice peptide. Background technique [0002] Rice is my country's largest agricultural product and grain variety. In 2008, my country's rice production accounted for about 30% of the world's rice production. Rice is the basic food for human beings to survive. Processing 100Kg of rice can produce 60-65Kg of rice, and the rest is 40Kg of by-products (rice husk, broken rice, rice bran and rice germ), and these by-products can be obtained after intensive processing. A high value-added product. [0003] For a long time, my country's rice processing is only in a primary processing stage to meet the demand for ration rice, and the deep processing of by-products is very little, and the resource utilization rate is low. With the rapid increase of population and the rapid depletion of resources, rice is an important food for human survival, and the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 唐波李舒宇仝志勇陈玉和
Owner 江苏联海生物科技有限公司
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