A shortcut method for artificial disease of tobacco bacterial wilt

Abstract

The invention discloses a method for fast manual disease attack of tobacco bacterial wilt disease. The method includes the following steps that first, tobacco seedlings are cultivated and prepared; second, bacteria solution for inoculation is cultivated and prepared; third, the tobacco seedlings are processed before being inoculated; fourth, bacterial wilt bacteria inoculation is performed; fifth, the tobacco seedlings are processed after inoculation. The method has the advantages that an inoculation system is miniature, inoculation is performed on miniature seedlings in miniature space, and the test cycle is short; the technological system is simple in process, convenient to operate, small in technical difficulty and high in working efficiency; the whole process is completed in a common laboratory, greenhouse equipment does not need to be arranged, local conditions of the disease attack can be controlled easily, interference is small, and standardization of a technological method can be achieved easily.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is a shortcut method for the artificial disease of tobacco bacterial wilt Background technique [0002] Tobacco bacterial wilt occurs globally and is a devastating disease in tobacco production. Many basic research and research on major theoretical issues related to disease prevention and control are inseparable from the basic method of inoculating pathogens, especially the pathogenic bacteria of the disease. (Ralstonia solanacearum) is currently one of the model systems for studying the pathogenic mechanism of plant pathogenic bacteria in the world. The research on this pathogen must frequently go through the process of pathogenicity testing, that is, the work of artificial inoculation. High-paced contemporary work often requires the means and methods used to be high-efficiency, high-throughput, and high-accurate. Therefore, efficient and fast artificial path...

AI Technical Summary

Problems solved by technology

Although these methods are very mature, effective and widely used, there are more or less disadvantages in their application as follows: 1) The test period is long. In the practical application of these methods and technologies, larger tobacco seedlings are usually used so far. Due to the The early growth is slow, and it takes a long time to cultivate and prepare tobacco seedlings, generally more than 2 months, which leads to a long test period

3) The technical requirements are relatively high. If injection vaccination is adopted, it generally requires quantitative injection plant by plant and point by point, which often requires the operator to pass the test and carefully operate, and the work efficiency is low in implementation

4) It is difficult to control some local conditions related to the disease. For example, it is required to create a humidity that is conducive to the disease after inoculation, which is still difficult under some common laboratory equipment conditions; the use of soil root injury inoculation is easily affected by other microorganisms in the soil. Although some small experiments can use sterilized or sterilized soil to grow seedlings, it is still difficult for general laboratories to provide full aseptic conditions during the seedling raising period, and the microbial population in the soil is still uncontrollable

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