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Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance

A technology for active parts and neuroprotection, applied in the field of medicine, can solve the problems that the active functional components are not very clear, and there is no refining of Cicada flower extracts.

Active Publication Date: 2014-05-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that cicadae and the anamorph of the famous and precious Chinese medicine Cordyceps sinensis belong to fungi (Cordyceps genus), and Paecilomyces cicadae has various pharmacological effects such as immunity, and has the advantages of low toxicity and easy cultivation, it is hopeful that it can be used as a substitute for Cordyceps sinensis. Its corresponding active functional components are not very clear, and most of the pharmacological experiments only stay on the basis of the original medicinal materials or crude extracts, and there is no report that the extract of Cicada japonica is further refined to the active site and used to prepare neuroprotective and anti-aging drugs

Method used

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  • Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance
  • Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance
  • Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The ethyl acetate extraction part (JCH AE )preparation:

[0017] Take 80 g of dried cicadae powder that has been crushed through the Pharmacopoeia No. 2 sieve, add 10 times of 80% ethanol for reflux extraction, and filter to recover ethanol. Cicada ethanol extract was dispersed in water, followed by an equal volume of petroleum ether, ethyl acetate, and fractional extraction of n-butanol, a total of 3 times of extraction, combined, concentrated under reduced pressure to recover the solvent, respectively to obtain the petroleum ether part, the ethyl acetate part ( JCH AE ), n-butanol part, water part, freeze-dried to get JCH AE 2.50g.

Embodiment 2

[0018] Example 2JCH AE Protective effect on glutamate-induced senescence of PC12 cells

[0019] (1) Determination of cell viability by MTT method and LDH method:

[0020] Take PC12 cells in the logarithmic growth phase (provided by Jiangsu University School of Medicine), and use 2 × 10 4 Inoculate in a 96-well culture plate at 200 μl / L per well. At 37°C, 5% CO 2 After culturing overnight under the condition, the cells were divided into control group, model group and drug treatment group. That is, the control group (complete medium without Glu); model group (complete medium + Glu); drug treatment group (complete medium + JCH AE +Glu, the concentrations were 200μg / mL, 100μg / mL, and 50μg / mL, diluted with culture medium. )JCH AE After pretreatment for 1 h, add Glu and incubate for 24 h. Set up 4 parallel wells in each group. After culturing for 24 hours, add 20 μl of 5g / L MTT to each well, continue culturing for 4 hours, then terminate the culture, carefully suck the supern...

Embodiment 3

[0037] Example 3JCH AE In vitro antioxidant activity assay:

[0038] (1) Determination of DPPH free radical scavenging ability:

[0039] Add 1.0mL of 200μmol / DPPH ethanol solution to 1mL of Cicada flower extract solution with different concentrations, then add 2.0mL of 80% ethanol and mix evenly. After placing it in a dark place for 30min, measure the absorbance value A at 517nm with a UV spectrophotometer 样品 , and simultaneously measure the absorbance value A of the mixture of 1.0mL DPPH ethanol solution and 3.omL ethanol solution 空白 and the absorbance value A of the mixture of 3mL ethanol and 1.0mL sample 对照 , clearance rate formula: DPPH clearance rate=[A 空白 -(A 样品 -A 对照 ) / A 空白 ]×100%. The data show that JCH AE Has the ability to remove DPPH·. The results are shown in Table 5

[0040] (2) Superoxide anion (O 2·- ) Determination of free radical scavenging ability:

[0041] Add 3ml Tris-HCl (PH8.2) to 1mL different concentrations of Cicada flower extract solution,...

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Abstract

The invention relates to a Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance. The active site is a Cordyceps sobolifera alcohol extract ethyl acetate extraction site. The active site is prepared by the following steps: pulverizing the medicinal material Cordyceps sobolifera, screening, extracting with ethanol under reflux, dispersing the ethanol extract with water, sequentially carrying out fractional extraction with petroleum ether, ethyl acetate and n-butanol for 3-5 times, merging, concentrating under reduced pressure to recover the solvent, and carrying out freeze-drying to respectively obtain a petroleum ether site, an ethyl acetate site, an n-butanol site and a water site. The ethyl acetate site can obviously inhibit aging and damage of the glutamic-acid-induced PC12 cells, prevent the cell LDH from release, enhance the survival rate of the cells, lower the intracellular oxygen free radical level, enhance the activity of the glutathione reductase (GSH-Px) and superoxide dismutase (SOD), and have favorable in-vitro oxidation resistance activity in the experiment of clearing DPPH. and superoxide anion (O<2.->) free radicals.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the preparation of parts with neuroprotective and anti-aging activities from Cicada japonica, a Chinese medicinal material, and its application in the preparation of neuroprotective and anti-aging drugs. Background technique [0002] With the aging population in our country, the incidence of neurodegenerative diseases is increasing day by day, and the research on aging and neurodegenerative diseases has become a neuroscience hotspot. The aging of the human body often leads to an accelerated reduction in the number of nerve cells, resulting in some neurodegenerative diseases represented by Alzheimer's Disease (AD). At present, the etiology of neurodegenerative diseases is unknown, and there is no effective treatment. Only a few drugs can be used to treat neurodegenerative diseases. [0003] Cicada is a dry complex formed by the fungus Cordyceps cicadae Shing of the f...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P25/00A61P39/06
Inventor 欧阳臻张魏琬麒赵明王吉标尚磊王璠汪愿
Owner JIANGSU UNIV
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