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Pleurotus eryngii var. tuoliensis and molecular marker identification method thereof

A technology of molecular markers and identification methods, applied in the field of identification of Bailing mushroom and its molecular markers, to achieve the effects of small error, simple operation, and intuitive judgment

Inactive Publication Date: 2014-05-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cultivation of Bailing mushroom is still affected by its relatively single species and its biological characteristics, such as long cultivation period and harsh cultivation conditions, which restrict the development of Bailing mushroom industry to a certain extent.

Method used

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  • Pleurotus eryngii var. tuoliensis and molecular marker identification method thereof
  • Pleurotus eryngii var. tuoliensis and molecular marker identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the screening of Bailing mushroom 54

[0027] Select 6 Pleurotus eryngii strains and 8 Pleurotus eryngii strains with excellent properties, and make original seeds after PDA solid medium is activated, and produce Pleurotus eryngii strains and Pleurotus eryngii strains according to the conventional cultivation methods of Pleurotus eryngii and Pleurotus eryngii. Mushroom test, the basidiospores were ejected, and the spores were germinated to obtain monospore strains of Bailing mushroom and Pleurotus eryngii. Selected 24 Pleurotus eryngii strains and 32 Pleurotus eryngii strains for one-one hybridization, a total of 768 groups were paired, and 116 hybrids of Pleurotus eryngii and Pleurotus eryngii were obtained, and 116 hybrids were obtained. Mushroom experiment, screen out the fruiting body shape and Bailing mushroom hybrid seed 54, named Bailing mushroom 54. Bailing mushroom 54 has been fruited many times, and its mushroom body is mussel-shaped or palm-sha...

Embodiment 2

[0028] Example 2: The fruiting test of Bailing mushroom 54 (CCTCC NO: M2013669), including the following steps:

[0029] 1. Production of Bailing mushroom 54 strains

[0030] The 54 strain of Bailing mushroom was activated twice on PDA culture, then transferred to the regular medium of Bailing mushroom original seed, and cultured at a constant temperature of 25°C until the original seed bottle was overgrown.

[0031] 2. Bailing mushroom 54 wall-type soil-covered cultivation management

[0032] The material selection, various raw material formulas, bagging, disinfection and sterilization, germination management and post-ripening management of Bailing mushroom 54 are basically the same as the routine. Mushroom production management refers to Zhang Zhixuan's Bailing mushroom wall-type soil-covered cultivation technology (Zhang Zhixuan. Bailing mushroom wall-type soil-covered cultivation technology [J]. Northern Horticulture 2010, 11: 189~190.)

[0033] The morphological charact...

Embodiment 3

[0034] Example 3: The molecular marker identification method of Bailing mushroom 54 (CCTCC NO: M2013669), including the following steps:

[0035] 1. Mycelia culture and collection

[0036] 1. Transfer Pleurotus eryngii 54, Pleurotus eryngii and Bailing mushroom bacterial strains to 250 ml wide-mouthed triangular flasks containing 100ml PDA liquid medium, wherein Pleurotus eryngii and Bailing mushroom are purchased in the market for reference;

[0037] 2. Place on a shaker at 25°C with a rotation speed of 90-130 r / min and incubate for 7-10 days;

[0038] 3. Filter the cultured mycelia with gauze, rinse with distilled water, and dry with filter paper;

[0039] 4. Weigh 0.5-1.3 g of mycelium, wrap it with filter paper, and store it at -20°C for later use.

[0040] 2. Genomic DNA extraction by CTAB method

[0041] 1. Take 0.5-1.3 g of the hyphae of the reserved hybrid strain and put it into a mortar, add liquid nitrogen to quickly grind it into powder, and transfer it to a 7ml ...

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Abstract

The invention belongs to the Pleurotus eryngii var. tuoliensis field and in particular relates to Pleurotus eryngii var. tuoliensis and a molecular marker identification method thereof. The Pleurotus eryngii var. tuoliensis 54 is preserved in China center for type culture collection in December 16th, 2013, and has the preservation number of CCTCCNO: M2013669. The molecular marker identification method of the Pleurotus eryngii var. tuoliensis 54 comprises the steps of mycelium culture and collection, genome DNA extraction, EF1alpha-PCR amplification, specific enzyme-digested fragment marker generation and electrophoresis detection of enzyme-digested products. The Pleurotus eryngii var. tuoliensis 54 has shorter cultivation period than common pleurotus nebrodensis, and has high yield and good development and application prospects; the molecular marker identification method has the characteristics of reality, reliability and clearness, has low error compared with the typical morphological identification method, is not influenced by environment conditions and can be judged intuitively.

Description

technical field [0001] The invention belongs to the field of Bailing mushroom, and more specifically relates to a Bailing mushroom and a molecular marker identification method thereof. Background technique [0002] The original Latin name of Bailing side ear Pleurotus nebrodensis (Inzengae) Quél, later Zhang Jinxia and others researched that the Latin name of Bailing mushroom cultivated in my country should be Pleurotus eryngii var. tuoliensis . Bailing mushroom is the trade name of Bailing side ear, also known as winged oyster mushroom, Bailingzhi mushroom, Kashmir mushroom, etc. Its distribution is similar to that of Xinjiang Ferula ( Ferula sinkiangensin The distribution of K.M.Shen) is closely related. In my country, it is only distributed in Altai, Toli, Mulei, Tacheng and other places in Xinjiang, and there are barren beaches where Xinjiang Ferulica grows. The distribution altitude is 720~1000m, and the genetic resources are limited. the progress of its breedi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/68C12Q1/04C12R1/645
Inventor 刘新锐谢宝贵邓优锦江玉姬朱坚
Owner FUJIAN AGRI & FORESTRY UNIV
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