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Composition for fermentation process of lipstatin

A technology of composition and solution, which is applied in the field of medicine and chemical industry, can solve the problem of low fermentation level, and achieve the effect of easy availability of raw materials and suitable production

Inactive Publication Date: 2014-06-04
鲁南新时代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation process involved in WO2007134836 and WO2007078263 partly adopts the process of feeding linoleic acid and amino acid, but the fermentation level is relatively low

Method used

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  • Composition for fermentation process of lipstatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 100mL of sunflower oil, put it in a 500mL beaker, stir and heat to 70°C, under constant temperature stirring, gradually add 1mol / L sodium hydroxide solution for saponification until the pH of the solution is 10.5, stop Add sodium hydroxide solution dropwise and keep warm for half an hour, cool down to 10°C, add 100mL petroleum ether, stir, let stand, filter, wash the filter cake once with 100mL petroleum ether, collect the filter cake and put it in a 500mL beaker, add 100mL water, Under stirring, add 20% hydrochloric acid solution dropwise until the pH is 2.5, let stand to separate layers, separate the water layer, wash the oil layer with water at 30°C until neutral, collect the oil layer to obtain the composition. Gas chromatography analysis results: stearic acid 2.2%, oleic acid 17.8%, linoleic acid 74.3%, palmitic acid 5.5%, other components 0.2%.

[0026] Adopt the method described in the inventor's existing patent CN102268466B to carry out slant cultivation, ...

Embodiment 2

[0028] Weigh 1L of sunflower oil, put it in a 5L beaker, stir and heat to 70°C, under constant temperature and constant speed stirring, gradually add 1mol / L sodium hydroxide solution for saponification until the pH of the solution is 11 , stop adding sodium hydroxide solution and keep it warm for half an hour, cool down to 10°C, add 1L petroleum ether to the above mixed solution, stir, let it stand, filter, wash the filter cake once with 1L petroleum ether, collect the filter cake and place it in 5L Add 1L of water into the beaker, under stirring, dropwise add 20% hydrochloric acid solution until the pH is 2.1, let stand to separate the layers, separate the water layer, wash the oil layer with 50°C water until neutral, collect the oil layer to obtain the composition. Gas chromatography analysis results: stearic acid 2.3%, oleic acid 17.7%, linoleic acid 73.7%, palmitic acid 5.8%. Other ingredients 0.5%.

[0029] According to the operation of Fermentation Example 1, the slant ...

Embodiment 3

[0031] Weigh 10L of sunflower oil, put it in a 50L reaction kettle, stir and heat to 70°C, under constant temperature and constant speed stirring, gradually add 1mol / L sodium hydroxide solution for saponification until the pH of the solution is 10.8 , stop adding sodium hydroxide solution dropwise and keep it warm for half an hour, cool down to 10°C, add 10L petroleum ether to the above mixed solution, stir, let it stand, filter, wash the filter cake once with 1L petroleum ether, collect the filter cake and place Add 10L of water into a 50L reactor, stir, add 20% hydrochloric acid solution dropwise until the pH is 2.0, let stand to separate layers, separate the water layer, wash the oil layer with water at 40°C until neutral, collect the oil layer to obtain the combination thing. Gas chromatography analysis results: stearic acid 2.3%, oleic acid 17.6%, linoleic acid 73.7%, palmitic acid 5.7%. Other ingredients 0.7%.

[0032]According to the operation of Fermentation Example ...

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Abstract

The invention belongs to the technical field of medicine chemical industry, and relates to a composition used in the fermentation process of lipstatin and preparation method of the composition. Specifically, the composition is obtained by saponifying sunflower seed oil into salt, separating the salt, acidizing and washing; the composition contains linoleic acid, and the cost is only a half of the linoleic acid on market, the linoleic acid in the fermentation process is replaced by the composition, and the fermentation valence is improved by 12-18%.

Description

technical field [0001] The invention relates to a composition used in the fermentation process of riprestatin and a preparation method thereof, belonging to the field of medicine and chemical industry. Background technique [0002] Liprestatin (compound of formula 1) is an intermediate of the new weight-loss drug orlistat. Orlistat is a long-acting and potent specific gastrointestinal lipase inhibitor that inactivates the enzymes by forming a covalent bond with the active serine sites of gastric and pancreatic lipases in the lumen of the stomach and small intestine To play a therapeutic role, inactivated enzymes cannot hydrolyze the fat in food, mainly triglycerides, into absorbable free fatty acids and monoacylglycerols. Undigested triglycerides cannot be absorbed by the body, thereby reducing caloric intake and controlling weight. [0003] [0004] Formula 1 [0005] There are two methods for the production of orlistat, fermentation synthesis and total synthesis. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02
Inventor 张太凤邵征杨雷陈杨
Owner 鲁南新时代生物技术有限公司
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