Blood glucose detection method and detection kit
A detection kit and detection method technology, applied in the field of medical diagnostic preparations, can solve the problems of shortened reaction time, interference, and long reaction time, and achieve the effects of wide application range, simple operation, and improved accuracy
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Embodiment 1
[0037] Embodiment 1, a kind of detection kit, comprises packing box, buffer solution, enzyme reagent, standard solution; Packing box is the outer packing of test box; The buffer solution is referred to as reagent 1 below, and the enzyme reagent is called reagent 2.
[0038] The buffer is 0.1 mol / L phosphate buffer containing 25 mmol / L p-hydroxybenzoate.
[0039] The enzyme reagent is glucose oxidase and peroxidase solution, the concentration of glucose oxidase is 5-200U / ml; the concentration of peroxidase is 2-30U / ml.
[0040] The volume ratio of reagent 1 to reagent 2 is 5-1:1.
[0041] The standard solution is a glucose solution with a concentration of 2-7mmol / L, which is calibrated with the national standard.
[0042] The storage conditions and validity period are: the original reagent is stored at 2-8°C, and the validity period is 12 months; after the buffer solution and enzyme reagent are made into a working solution, it can be stable at room temperature for 8 hours, and...
Embodiment 2
[0062] Embodiment 2, a detection kit, except that the following is different from Example 1, all the other are the same as the detection kit in Example 1; the buffer is 0.1mol / L phosphate buffer, containing 25mmol / L p-hydroxybenzene Formate, the pH value is 7.3; the enzyme reagent is glucose oxidase solution, the concentration is 10U / ml, containing 5U / ml peroxidase and 3mmol / L 4-aminoantipyrine; the volume of reagent 1 and reagent 2 The ratio is 4:1; the standard solution is a blood glucose solution with a concentration of 5.55mmol / L, which is calibrated with the national standard.
[0063] Use the above detection kit to detect blood glucose. The detection method is carried out according to the following steps: the absorbance rise rate measurement step is carried out according to the double reagent mode operation. First, take the required blank tube, standard tube and sample tube according to the measurement, and add buffer respectively. ; Then add the sample to be tested, dis...
Embodiment 3
[0080] Embodiment 3, a detection kit, except that the following is different from Example 1, all the other are the same as the detection kit in Example 1; the buffer is 0.1mol / L phosphate buffer, containing 40mmol / L p-hydroxybenzene Formate, pH value is 7.3; Enzyme reagent is glucose oxidase solution, concentration is 12U / ml, contains the 4-aminoantipyrine of 2U / ml peroxidase and 1.2mmol / L; Reagent 1 and reagent 2 The volume ratio is 1:1; the standard solution is blood glucose solution with a concentration of 5.55mmol / L, which is calibrated with the national standard.
[0081] Use the above detection kit to detect blood glucose. The detection method is carried out according to the following steps: the absorbance rise rate measurement step is carried out according to the double reagent mode operation. First, take the required blank tube, standard tube and sample tube according to the measurement, and add buffer respectively. ; Then add the sample to be tested, distilled water a...
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