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A cultivation method and technology of kelp, applied in seaweed cultivation, botanical equipment and methods, cultivation and other directions, can solve the problems of high labor intensity, difficult to control conditions, and high investment cost, and achieve labor saving, convenient operation, and high cultivation yield. Effect
Active Publication Date: 2016-08-17
海宁经开产业园区开发建设有限公司
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PCT patent 00812730.1 discloses an improved method of cultivating seaweed, which uses antibiotics and fungicides to aseptically treat the growable seaweed material, and then isolates the healer on a PES-enhanced agar plate with controlled temperature and light for 40 days. Injure the tissue, adjust the temperature and light to cultivate asexual buds, and then transfer to liquid PES medium to cultivate and develop into immature embryos with multiple branches under vibration conditions, and finally cultivate them in perforated polyethylene bags to realize large-scale cultivation of seaweed; Chinese patent 201120053539.5 discloses a ventilated culture bottle for seaweed cultivation, which has a ventilation tube with an upward opening through the vent hole at the bottom of the culture bottle, and the mouth of the culture bottle is provided with a filter membrane to facilitate the introduction of gas , which improves the success rate of seaweed cultivation; but the seaweed cultivation methods and devices disclosed in the above-mentioned patented technology all have the disadvantages of complex process, difficult conditions, high investment cost, and high labor intensity. Therefore, the present invention seeks to design An easy-to-implement, high-speed, high-efficiency seaweed cultivation method has good application prospects and implementation value
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Embodiment 1
[0012] The present embodiment is illustrated by taking laver somatic cell culture as an example, and the specific method includes the following process steps:
[0013] (1) Put the natural seawater into the seawater tank, control the temperature at 70-90°C, and irradiate with ultraviolet light for 2-5 hours for heat disinfection treatment before standby;
[0014] (2) Dry the selected and collected species of laver until the water content of the cells is below 20%, culture in the culture medium at a temperature controlled 15-20°C under low light for 4-5 days, and then place them in a medium containing conch enzyme, abalone enzyme and agarase In the enzyme solution, the temperature is controlled at 23-28°C, the pH value is 5.6-6.4, and the enzymolysis treatment is carried out, and then the enzyme solution and cells are filtered with a 30-60um pore size sieve, and the porphyra somatic cells are collected by centrifugation for later use;
[0015] (3) Place the Porphyra somatic cell...
Embodiment 2
[0018] The present embodiment is illustrated by taking the culture of kelp gametophytes as an example, and the specific method includes the following process steps:
[0019] (1) Put the natural seawater into the seawater tank, control the temperature at 70-90°C, and irradiate with ultraviolet light for 2-5 hours for heat disinfection treatment before standby;
[0020] (2) Select kelp with thick, shiny, strong algae and large sporangia as the seed kelp, wash the seed kelp with the sterilized seawater in step (1) for 3-5 times, and place it in step (1) In the conventional artificial medium of seawater after neutralization and disinfection, the temperature is controlled at 15-19°C, and the sunlight is 500-4000Lx. After 6-10 days of cultivation, zoospores are formed, and after the temperature is raised to 18-21°C for 5-10 days, female gametophytes are formed. with the male gametophyte;
[0021] (3) Adjust the temperature to 10-15°C, use long-term light, and continue to cultivate ...
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Abstract
The invention belongs to the technical field of seaweed cultivation, and relates to a seaweed cultivation method. Natural seawater is contained in a seawater tank, and the temperature and the ultraviolet lamp irradiation are controlled to conduct disinfection treatment for standby application; selected and collected seed seaweeds are washed, disinfected and dried, then conventional culture media are selected according to different seaweed types, temperature control and dim-light static cultivation are carried out, and then enzymolysis or direct cultivation is carried out; after to-be-cultivated somatic cells, to-be-cultivated gametophytes or to-be-cultivated callus mature, bolting-silk filtering, sectioning processing and / or centrifugal collecting are carried out, the somatic cells, the gametophytes or the callus are placed into the culture media according to the requirements of different kinds of seaweeds, the temperature, the illumination and the osmotic pressure are controlled for cultivation, and the somatic cells, the gametophytes / sporophores and the callus of the seed seaweeds are attached to the silk net faces in the culture media; silk nets are then manually scattered into the offshore or the tidal zone to conduct conventional seawater cultivation. The seaweed cultivation method is simple in technology, low in cost, convenient to operate, short in seedling growing time, good in cultivation effect and high in cultivation yield, and manpower is saved.
Description
Technical field: [0001] The invention belongs to the technical field of seaweed cultivation, and specifically relates to a method for cultivating seaweeds. According to the characteristics of different types of seaweeds, tissues or protoplasts are selected to realize the cultivation and cultivation of large seaweeds. Background technique: [0002] Seaweed is an algae plant born in the sea. It generally includes red algae, green algae, brown algae, cyanobacteria, diatoms and potassium algae. The common ones are kelp, asparagus and laver, all of which are rich in protein and polysaccharides. , dietary fiber, vitamins, inorganic elements, amino acids and fatty acids, etc., have good food value, industrial value and medicinal value. In the past few decades, with the successful application of tissue culture technology in higher plants, the tissue culture of macroalgae has gradually attracted people's attention, but the living environment of seaweed is complex and different from t...
Claims
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Application Information
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