Lysobacter enzymogenes mutant strain and preparation method thereof

Abstract

The invention provides a lysobacter enzymogenes mutant strain TGJZC-041. The method comprises the following steps: inoculating the bacteria into a nutrient agar culture-medium slope; standing and culturing at 30+ / -1 DEG C for 2-3 days; inoculating into the fermentation medium at the inoculum size of 5-10%; carrying out shake culturing in a constant temperature shaker at 30+ / -1 DEG C at the rotating speed of 215-225rpm for 3 days, so as to prepare an incision enzyme for lysine peptide. The lysobacter enzymogenes mutant strain has the beneficial effects that the Lys-C generated by the mutant strain is relatively high in expression quantity and shorter in fermentation period.

Description

technical field

[0001] The invention belongs to the field of mutation breeding, in particular to a method for space mutation breeding of lysobacterium enzymogenes and a mutant strain of lysobacterium enzymogenesis obtained by space mutation breeding. Background technique

[0002] Lysine endopeptidase Lys-C (Endoproteinase Lys-C) is an alkaline protease with a molecular weight of about 30kD and belongs to a member of the serine protease family. It can specifically hydrolyze the C-terminus of lysine residues in proteins or peptide chains, and can also be used for protein identification by peptide mass fingerprinting or MS / MS spectral matching, so it has a good application in proteomics analysis prospect. In addition, Lys-C can also be used for the cleavage of precursors in the industrial production of recombinant proteins (such as insulin and its analogues). Compared with commonly used trypsin, Lys-C has several significant advantages: 1. Strong specificity, enzyme High acti...

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