Endocervical cell preserving fluid and method for preparing endocervical cell specimen

Abstract

The invention discloses endocervical cell preserving fluid and a method for preparing an endocervical cell specimen. The endocervical cell preserving fluid comprises N-acetyl-3-cysteine, anhydrous alcohol, sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, glacial acetic acid, concentrated hydrochloric acid and distilled water. The method for preparing the endocervical cell specimen comprises the following steps; soaking endocervical cells in a collection tube of the endocervical cell preserving fluid, uniformly oscillating, adding the endocervical cells in a smear device, arranging the smear device in a centrifugal machine for centrifuging, and taking a glass slide with a uniform thin-layer cell smear; and fixing the obtained glass slide with the cell smear by a fixing solution, dyeing, dehydrating, making the glass slide transparent, and finally, mounting to obtain a specimen smear for diagnosis. The endocervical cell preserving fluid and the method for preparing the endocervical cell specimen are reliable and practical, are low in cost, and have useful effects, thus being suitable for large-scale popularization.

Description

technical field [0001] The invention relates to a cervical cell preservation solution and a preparation method of cervical cell specimens. Background technique [0002] Cervical cancer is one of the most common gynecological malignancies, so the screening of cervical cancer has been widely concerned. The traditional Pap direct smear method is the main method for early screening of cervical cancer and has been used for more than 50 years. However, the traditional Pap direct smear method has the following disadvantages: the sample collection uses cotton swabs or cork chips, which is easy to contaminate transport personnel and tools; only 20% of the cells collected by direct smear are applied on the glass slide, and there are too few diagnostic components of the cells; Excessive force during filming will deform the cells; it is easy to dry the film, causing cell rupture and making it difficult to diagnose; discarding the sampler, about 80% of the cell samples are left on the s...

AI Technical Summary

Problems solved by technology

The traditional Pap direct smear method is the main method for early screening of cervical cancer and has been used for more than 50 years. However, the traditional Pap direct smear method has the following disadvantages: the sample collection uses cotton swabs or cork chips, which is easy to contaminate transport personnel and tools; only 20% of the cells collected by direct smear are applied on the glass slide, and there are too few diagnostic components of the cells; Excessive force during filming will deform the cells; it is easy to dry the film, causing cell rupture and making it difficult to diagnose; discarding the sampler, about 80% of the cell samples are left on the sampler and discarded, and abnormal cells that may be found are also discarded; Cell specimens cannot be preserved and cannot be made into slides; a large amount of mucus, red blood cells, and white blood cells cover the smear, resulting in unclear background and poor staining of the smear, which is not conducive to diagnosis and affects the detection rate to a certain extent

However, the "cervical cell preservation solution" used in this technology is mostly imported from abroad, the price is very expensive, the source is inconvenient, and it cannot be popularized, so it cannot be used for extensive cervical cancer screening.

The cytocentrifuge or membrane-type ultra-thin cell slicer in this technology is also very expensive, and some small and medium-sized hospitals cannot afford it.

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