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Injectable silk fibroin-alginate double cross-linking hydrogel and preparation method and use method thereof

A technology of alginate and hydrogel, applied in medical science, prosthesis, etc., can solve the problem of not being able to prepare injectable silk fibroin-alginate hydrogel, and achieve good biodegradability and gelation speed Fast, simple process effect

Inactive Publication Date: 2014-06-25
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Adopt above-mentioned method, all can not prepare injectable silk fibroin-alginate hydrogel

Method used

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  • Injectable silk fibroin-alginate double cross-linking hydrogel and preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of solution A silk fibroin protein solution: cut silkworm cocoons into pieces, add 0.05 mol / L Na 2 CO 3 Aqueous solution with a bath ratio of 1:200, heated to boiling and kept for 60 min to remove sericin on the surface of silk. After washing and drying, the silk fibroin was dissolved in CaCl 2 / EtOH / H 2 O (substance ratio: 1 mol:2 mol:8 mol) dissolved in the system, placed in running water and dialyzed for 3 days to remove ethanol and free Ca in the solution 2+ , prepare 10 mL of silk fibroin protein solution with a mass concentration of 1 wt% with deionized water, stir and add 0.0451 g of CaCl 2 .

[0040] Preparation of solution B modified alginate solution: Dissolve alginate, prepare 10 mL of alginate solution with a mass concentration of 2 wt% in deionized water, add a cross-linking agent 1-ethyl - 0.0052 g of 3-(3-dimethylaminopropyl) carbodiimide (EDC), 0.0016 g of N-hydroxysuccinimide (NHS), the pH of the solution was 7.0, stirred, and aged for ...

Embodiment 2

[0043] Preparation of solution A: Prepare 10 mL of silk fibroin protein solution with a mass concentration of 1 wt% according to the method described in Example 1, stir and add 0.0618 grams of CaCl 2 .

[0044] Preparation of Solution B: Prepare according to the steps described in Example 1.

[0045] Cross-linking: according to the steps described in Example 1, a double-cross-linked hydrogel with amino and carboxyl covalent cross-linking and metal ion cross-linking was prepared. As the concentration of calcium ions increases, the gelation time of the obtained hydrogel decreases to 100 s; the cell size of the hydrogel decreases, and the cell distribution is uniform; the elastic modulus increases to 700-900Pa, and the water swelling rate decreases. Small, the degradation rate is reduced.

Embodiment 3

[0047] Preparation of Solution A: Follow the steps described in Example 1.

[0048] Preparation of solution B: Prepare 10 mL of alginate solution with a mass concentration of 2 wt% as described in Example 1, and add a cross-linking agent 1-ethyl-3-(3-dimethylammonia Propyl) carbodiimide (EDC) 0.0104 g, N-hydroxysuccinimide (NHS) 0.0031 g, solution pH 7.0, stirred, and aged for 100 min.

[0049] Crosslinking: Proceed as described in Example 1.

[0050] Increasing the concentration of carboxyl-activated cross-linking agent increases the gelation time of the obtained hydrogel to 240 s, increases the elastic modulus, decreases the water swelling rate of the hydrogel, and decreases the degradation rate.

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Abstract

The invention relates to injectable silk fibroin-alginate double cross-linking hydrogel which comprises a solution A: a silk fibroin solution and a solution B: a modified alginate solution, wherein the solution A comprises the following components in parts by weight: 1.0*10<-1>-3.0 parts of silk fibroin, 1.4*10<-3>-2.0*10<-3> part of a divalent metal ion cross-linking agent, and the balance of water containing 1-5wt% of silk fibroin; the solution B comprises the following components in parts by weight: 5.0*10<-1>-4.0 parts of alginate, 2.5*10<-3>-4.0*10<-1> part of a carboxyl activating cross-linking agent and the balance of water containing 1-5wt% of alginate. By adopting a double cross-linking method of covalent cross-linking and ion cross-linking, the double cross-linking hydrogel is quick to polymerize in situ and has an application prospect in the fields such as engineering stents, drug controlled release and regenerative medicines. The invention further provides a preparation method and a use method of the double cross-linking hydrogel.

Description

technical field [0001] The invention belongs to biomedical materials, and specifically relates to the processing and utilization technology of silk fibroin, more specifically, an injectable silk fibroin-alginate double cross-linked hydrogel and its preparation method and use method. Background technique [0002] The loss and dysfunction of tissues and organs is one of the most important reasons that affect the quality of human life and lead to human disease and death. The repair and reconstruction of damaged organs is a major problem in biology and clinical medicine. Tissue engineering uses scaffold materials, seed cells, and growth factors to regenerate tissues and organs in vitro in bioreactors or in vivo. Scaffold materials for tissue repair are one of the central contents of tissue engineering and regenerative medicine. In terms of physical properties, it is the space for seed cells to attach and survive, providing spatial architecture, mechanical requirements and geom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/52C08J3/075C08J3/24
Inventor 沈星灿李子恒纪仕辰王亚珍覃山福
Owner GUANGXI NORMAL UNIV
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