Effective method for preparing avermectin

A technology of abamectin and Streptomyces avermitilis, applied in the field of microorganisms, can solve the problems of low yield of production strains, backward fermentation technology and the like, and achieve the effects of improving efficiency and increasing yield

Inactive Publication Date: 2014-06-25
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In my country, the development, research and industrial production of abamectin has been listed as a national key scientific and technological project, and several pharmaceutical companies have been producing abamectin. However, the production enterprises of abamectin in my country The yield of strains is low, and the fermentation technology is backward

Method used

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  • Effective method for preparing avermectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Use 10 500mL Erlenmeyer flasks, each containing 150mL medium A (corn starch 3.0%, α-amylase 6u / 100mL, soybean meal powder 0.8%, yeast extract 0.4%, peanut cake powder 1.0%, cobalt chloride 0.3%), Sterilize at 120° C. for 30 minutes, inoculate the activated Streptomyces avermitilis (Streptomyces avermitilis) AW-H1 bacteria liquid after cooling, place at 28° C., and shake the flask for 32 hours. Inoculate the cultured strain solution into a 50L fermenter with 20L sterilized medium B (medium B: corn flour 0.04%, soluble starch 0.01%, cornstarch 14.0% , soybean powder 0.008%, soybean meal powder 2.8%, peanut cake powder 0.006%, yeast extract 0.008%, yeast powder 1.0%, manganese sulfate 0.0072%, ammonium sulfate 0.025%, dipotassium hydrogen phosphate 0.0008%, cobalt chloride 0.002%, Sodium chloride 0.0003%, sodium molybdate 0.023%, α-amylase 28u / 100mL, calcium carbonate 0.08%), at 27°C-32°C, aeration and stirring fermentation for 8-20 hours, with 0.01-1.0L / Sodium propionat...

Embodiment 2

[0051] Use 12 500mL Erlenmeyer flasks, each containing 150mL medium A (corn starch 4.0%, α-amylase 8u / 100mL, soybean meal powder 1.0%, yeast extract 0.4%, peanut cake powder 0.8%, cobalt chloride 0.2%), Sterilize at 120° C. for 30 minutes, inoculate the activated Streptomyces avermitilis (Streptomyces avermitilis) AW-H1 bacteria liquid after cooling, place at 28° C., and shake the flask for 32 hours. Inoculate the cultured strain solution into a 50L fermenter with 20L sterilized medium B (medium B: 0.08% corn flour, 0.02% soluble starch, 12.0% cornstarch) , soybean powder 0.02%, soybean meal powder 3.0%, peanut cake powder 0.01%, yeast extract 0.01%, yeast powder 1.5%, manganese sulfate 0.0024%, ammonium sulfate 0.02%, dipotassium hydrogen phosphate 0.001%, cobalt chloride 0.0025%, Sodium chloride 0.001%, sodium molybdate 0.025%, α-amylase 30u / 100mL, calcium carbonate 0.08%), at 27°C-32°C, aeration and stirring fermentation for 8-20 hours, intermittent feeding of propionic aci...

Embodiment 3

[0056] Use 10 500mL Erlenmeyer flasks, each containing 150mL medium A (corn starch 4.0%, α-amylase 8u / 100mL, soybean meal powder 1.0%, yeast extract 0.5%, peanut cake powder 1.0%, cobalt chloride 0.2%), Sterilize at 120°C for 30 minutes, inoculate the activated Streptomyces avermitilis AW-12 (preserved in our laboratory) bacterial solution after cooling, place at 28°C, and shake the flask for 32 hours. Inoculate the cultured strain solution into a 50L fermenter with 20L sterilized medium B (medium B: 0.06% corn flour, 0.008% soluble starch, 16.0% cornstarch, Soybean powder 0.01%, soybean meal powder 3.0%, peanut cake powder 0.02%, yeast extract 0.02%, yeast powder 1.5%, manganese sulfate 0.003%, ammonium sulfate 0.025%, dipotassium hydrogen phosphate 0.002%, cobalt chloride 0.002%, chlorine Sodium chloride 0.0008%, sodium molybdate 0.023%, α-amylase 40u / 100mL, calcium carbonate 0.1%), at 27°C-32°C, aeration and stirring fermentation for 8-20 hours, then 0.01-1.0L / h Sodium pro...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a method for preparing avermectin (Avermectins). The method comprises the following steps: cultivating a streptomyces avermitilis strain capable of generating avermectin and genetic improved bacteria thereof in a first stage of fluid nutrient medium, and then inoculating into a second stage of fluid nutrient medium as a seed solution; fermenting and cultivating after inoculating the seed solution into the second stage of fluid nutrient medium, and feeding a sodium propionate precursor to carry out feeding cultivation; collecting the avermectin B1a from fermentation broth after fermentation is finished. The effective method has the characteristics of being high in production efficiency and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an effective method for preparing abamectin. Background technique [0002] Avermectin is a group of 16-membered macrolide antibiotics with similar structure synthesized by the secondary metabolism of Streptomyces avermitilis. It has broad-spectrum and high-efficiency insecticidal and acaricidal activities. Composed of 8 components, namely Abamectin A1a, A2a,, B2a, A1b, A2b, B1b, B2b, wherein, Abamectin B1a has the highest activity. [0003] Due to its high activity, wide insecticidal spectrum, no persistent residue, environmental safety, unique chemical structure and easy modification, abamectin has become a hot spot in the development of biological pesticides. In 1985, Abamectin was officially introduced to the market as an agricultural insecticide, and it has been widely used in the prevention and control of plant pests and mites in vegetables, fruit trees, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/465
Inventor 钟娟谭红周金燕舒丹罗笛杨杰肖亮
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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