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Application of ginkgo seed extract

A technology of extracts and ginkgo kernels, which can be used in the fields of medicine, food or health care products, can solve problems such as aplastic anemia, colchicine with large side effects, leukopenia, etc., and achieve strong inhibitory effect and good anti-gout effect Effect

Inactive Publication Date: 2014-07-02
HUZHOU R & D CENT FOR NUTRITION & HEALTH SHANGHAI INST FOR BIOLOGICAL SCI CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The side effects of colchicine are relatively large, such as nausea, vomiting, diarrhea and other gastrointestinal reactions are common, and severe cases can lead to leukopenia, aplastic anemia, abnormal liver function, etc.; non-steroidal drugs can cause gastrointestinal tract, kidney, central nervous system, skin, liver, etc.; uric acid excretion drugs, such as probenecid, can cause kidney damage and kidney stones, etc., while benzbromarone can cause gastrointestinal reactions, kidney stones, renal colic, and induce acute attacks of arthritis etc.; xanthine oxidase inhibitors, such as allopurinol, which is a purine analogue, was marketed in the 1960s. Although it has been used until now, it also has many side effects. Patients may have fever, allergic rash, and abdominal pain. , diarrhea, leukopenia and thrombocytopenia, and even liver damage and other side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 , the preparation of ginkgo kernel extract

[0025] Appropriate amount of coarse powder of medicinal materials, 10 times the amount of 70% ethanol, heated and refluxed for 3 times, 2h each time, filtered and combined the filtrate, concentrated the filtrate under reduced pressure, and dried the concentrated solution to obtain the extract powder.

Embodiment 2

[0026] Example 2 Inhibitory effect of ginkgo kernel extract on xanthine oxidase

[0027] Experimental Materials:

[0028] Drug to be tested: ginkgo kernel extract (sample prepared in Example 1), dissolved in DMSO to prepare a 60 mg / mL mother solution.

[0029] Xanthine oxidase: Sigma, Cat.:X1875

[0030] Xanthine: Sigma, Cat.: X7375

[0031] Positive control drug: Allopurinol (Jiangsu Fangqiang Pharmaceutical)

[0032] experimental method:

[0033] Potassium phosphate buffer was used as the diluent to prepare different working concentrations of drug solution, xanthine oxidase (0.04U / mL) and xanthine solution (0.8mM), and 50 μL of drug solution and 50 μL of yellow purine solution were added to a 96-well assay plate. Purine oxidase and 100 μL xanthine solution were detected with a microplate reader at 290 nm, and the values ​​of absorbance and absorbance increase were recorded every 5 seconds for a total of 10 minutes.

[0034] The experimental results are shown in Table ...

Embodiment 3

[0038] Example 3, the inhibitory effect of ginkgo kernel extract on tumor necrosis factor alpha

[0039] Experimental Materials:

[0040] Drug to be tested: ginkgo kernel extract (sample prepared in Example 1), dissolved in DMSO to prepare a 60 mg / mL mother solution.

[0041] TNFαELISA Kit: R&D, Cat.:DY210

[0042] Positive control drug: IKK-2 inhibitor IV (Santa cruz, Cat.:sc-203083)

[0043] Lipopolysaccharide (LPS): Sigma, Cat.:L6529

[0044] experimental method:

[0045] Fresh healthy adult whole blood was taken, and human peripheral blood mononuclear cells (PBMC) were separated by density gradient centrifugation. After counting, they were seeded in 96-well cell culture plates, and drugs of different concentrations were added. After pretreatment for 30 minutes, lipopolysaccharide (LPS) stimulation overnight, the next day, according to the method of TNFαELISA kit ELISA test detection of TNFα protein content.

[0046] The experimental results are shown in Table 2 below:...

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PUM

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Abstract

The invention discloses application of a ginkgo seed extract in preparation of anti-gout medicaments, foods or healthcare products. The study finds that the ginkgo seed extract has a strong inhibiting effect on xanthine oxidase and tumor necrosis factor alpha. The ginkgo seed extract disclosed by the invention has a good anti-gout effect, and can be applied to preparation of the anti-gout medicaments, foods or healthcare products and pharmaceutical compositions. New use is developed for application of the ginkgo seed extract.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to the application of ginkgo kernel extract for preparing anti-gout medicine, food or health products. Background technique [0002] Gout is a group of abnormalities caused by the disorder of purine metabolism in the body, increased synthesis or decreased excretion of uric acid in the body, resulting in increased blood uric acid. Clinically, it often manifests as hyperuricemia, recurrent gouty acute arthritis, and the formation of tophi. In severe cases, it can cause joint deformity and dysfunction or gouty nephropathy. Xanthine oxidase is an enzyme that can not only catalyze hypoxanthine to xanthine, and then generate uric acid, but also directly catalyze xanthine to generate uric acid. Studies have found that inhibitors targeting xanthine oxidase have good anti-gout application prospect. [0003] Anti-gout drugs commonly used clinically include colchicine,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P19/06A23L1/29A61K131/00A23L33/00
Inventor 王滔王永兵魏晨宗伟英肖功胜蒋文丹
Owner HUZHOU R & D CENT FOR NUTRITION & HEALTH SHANGHAI INST FOR BIOLOGICAL SCI CHINESE ACADEMY OF SCI
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