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Edible peanut protein isolate antimicrobial coating and preparation method thereof

A peanut protein isolate and antibacterial film technology, applied in flexible coverings, transportation and packaging, packaging, etc., to achieve the effects of accelerating industrial production, accelerating practical commercial applications, improving film-forming properties and anti-corrosion and fresh-keeping capabilities

Inactive Publication Date: 2014-07-23
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no literature report using peanut protein isolate and chitosan as film-forming materials, adding clove essential oil and Nisin to the film-forming solution to produce peanut protein isolate edible film

Method used

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  • Edible peanut protein isolate antimicrobial coating and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 3g of peanut protein isolate, dissolve it in 50mL of distilled water, adjust the pH to 9.0 with 0.1N NaOH, stir and dissolve to form a peanut protein isolate solution; weigh 1g of chitosan and dissolve it in 50mL of 2% (V / V) acetic acid solution, stir Dissolved into chitosan solution; mix the two solutions, add 2mL glycerin as a plasticizer, stir evenly with a glass rod, put in an ultrasonic water bath at 75°C for 30min, then cool it to 40°C, add 4mL clove essential oil, and use a homogenizer After homogenizing the above solution for 1 min, put it into a vacuum degasser for degassing 3-4 times, take 50mL of film-forming liquid and pour it on a 20cm×20cm plexiglass plate, cast it, and place it in a blast drying oven at 50°C After internal drying for 10 hours, the film was peeled off, and the peeled film was placed in a desiccator with a relative humidity of 52% (filled with saturated magnesium nitrate solution to maintain its humidity), and its film-forming performa...

Embodiment 2

[0033] Weigh 3g of peanut protein isolate, dissolve it in 50mL of distilled water, adjust the pH to 9.0 with 0.1N NaOH, stir and dissolve to form a peanut protein isolate solution; weigh 1g of chitosan and dissolve it in 50mL of 2% (V / V) acetic acid solution, stir Dissolved into chitosan solution; mix the two solutions, add 2mL glycerin as a plasticizer, stir evenly with a glass rod, put in an ultrasonic water bath at 75°C for 30min, then cool it to 40°C, add Nisin at 5000IU / mL, use Homogenizer Homogenize the above solution for 1min, put it into a vacuum degasser for degassing 3-4 times, take 50mL of film-forming liquid and pour it on a 20cm×20cm plexiglass plate, cast it, and blow it at 50°C After drying in a drying oven for 10 hours, peel off the film, put the peeled film in a desiccator with a relative humidity of 52% (filled with saturated magnesium nitrate solution to maintain its humidity), and measure its film-forming performance and antibacterial effect after equilibrat...

Embodiment 3

[0035] Weigh 3g of peanut protein isolate, dissolve it in 50mL of distilled water, adjust the pH to 9.0 with 0.1N NaOH, stir and dissolve to form a peanut protein isolate solution; weigh 1g of chitosan and dissolve it in 50mL of 2% (V / V) acetic acid solution, stir Dissolved into a chitosan solution; mix the two solutions, add 2mL glycerin as a plasticizer, stir evenly with a glass rod, put in a 75°C ultrasonic water bath for 30min, then cool it to 40°C, add 4mL clove essential oil and press 5000IU / Add Nisin to mL, homogenize the above solution for 1min with a homogenizer, put it into a vacuum degasser for degassing 3-4 times, take 50mL of film-forming liquid and pour it on a 20cm×20cm plexiglass plate, and tape-cast it. After drying in a blast drying oven at 50°C for 10 hours, peel off the film, place the peeled film in a desiccator with a relative humidity of 52% (filled with saturated magnesium nitrate solution to maintain its humidity), and measure its film formation after ...

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PUM

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Abstract

The invention discloses an edible peanut protein isolate antimicrobial coating and a preparation method thereof. The method comprises the following steps: (1) dissolving peanut protein isolate; (2) dissolving chitosan; (3) uniformly mixing a peanut protein isolate solution with a chitosan solution; (4) adding glycerin as a plasticizer; (5) promoting the dissolution by use of an ultrasonic water bath; (6) adding a clove essential oil component and a nisin component; (7) homogenizing; (8) degassing; (9) coating; and (10) drying and uncovering the coating. The antimicrobial coating disclosed by the invention not only can be used for preferably preventing migration of oxygen, carbon dioxide and water vapor but also is nontoxic and harmless to a human body and edible.

Description

technical field [0001] The invention relates to an edible peanut protein isolate antibacterial film and a preparation method thereof, belonging to the application technical field of antibacterial packaging films. Background technique [0002] In recent years, more and more people pay attention to the environment-friendly biopolymer materials made of natural polymers. Among them, edible film, as a new type of packaging material, has the advantages of extensive raw material sources, edible, and pollution-free, so it has become a hot spot in the research of food, medicine, and packaging. Widespread concern. [0003] Peanut protein is a kind of vegetable protein with high quality, low price and abundant sources. There are a large number of hydrogen bonds, hydrophobic bonds, van der Waals forces, ionic bonds, and coordination bonds in peanut protein molecules, which make peanut protein have better film-forming properties. However, so far, there are few industrialized productio...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L5/08C08K5/00C08J5/18B65D65/38
Inventor 杨伟强李鹏孙杰江晨任艳彭娅萍张玉凤焦坤宫清轩
Owner QINGDAO AGRI UNIV
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