Method for testing hemoglobin concentration by utilizing liquid chromatogram tandem mass spectrum

Abstract

The invention relates to the technical field of apparatus analysis, and in particular relates to a method for testing hemoglobin concentration by utilizing liquid chromatogram tandem mass spectrum. The method comprises the following steps of: A, decomposing a hemoglobin sample to obtain a splitting segment of a globin polypeptide chain; B, separating the splitting segment to obtain one or more splitting segments serving as marking peptide segments, and quantitatively testing by utilizing mass spectrum; C, setting standard hemoglobin concentration gradient, building a linear regression equation of mass spectrum response intensity and hemoglobin concentration according to the existing hemoglobin concentration testing method, introducing a sample mass spectrum response intensity value into an equation for calculating to obtain hemoglobin concentration of the sample. Because whole hemoglobin molecules are not directly tested, the hemoglobin concentration of specimen during collection still can be accurately tested under the conditions without any earlier stage processing and with long storage time.

Description

technical field [0001] The invention relates to the technical field of instrumental analysis, in particular to a method for measuring the hemoglobin concentration in blood by using liquid chromatography and mass spectrometry, especially capable of measuring the hemoglobin concentration of trace blood samples stored for a long time. Background technique [0002] Hemoglobin is a functional protein in red blood cells. Oxygen is bound to iron atoms and transported by blood. Hemoglobin plays the role of transporting oxygen and decomposing H in the living body 2 o 2 , transfer electrons and other important activities related to oxygen and energy metabolism. Because leukemia, anemia and heart disease are often accompanied by abnormal hemoglobin clinically, the determination of hemoglobin content in human serum is an important indicator for evaluating human health. [0003] It is known that hemoglobin (Hemoglobin, Hb) is composed of one globin and four heme (also known as ferrous...

AI Technical Summary

Problems solved by technology

Since the above-mentioned existing methods are all based on the detection of biological activity or physical and chemical properties under the condition of intact hemoglobin structure, when the blood sample is kept for too long, hemolysis or hemoglobin cracking may occur, and the result of the measurement is difficult to reflect the hemoglobin at the time of sample collection. The real concentration, and the experimental operation is cumbersome, and the test efficiency is low

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