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Bacillusamyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, which is applied in the fields of application, bacteria, and climate change adaptation. It can solve the problems that the beneficial effect is difficult to effectively exert, and achieve good commercial prospects, simple production process, and wide antibacterial spectrum.

Active Publication Date: 2014-08-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the probiotics currently used in aquaculture come from terrestrial environments or terrestrial animals. Most of these probiotics are not suitable for growth and reproduction in aquatic environments, especially marine environments, making it difficult to effectively exert their beneficial effects

Method used

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  • Bacillusamyloliquefaciens and application thereof
  • Bacillusamyloliquefaciens and application thereof
  • Bacillusamyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of dry bacterial powder of Bacillus amyloliquefaciens M001:

[0040] The strains preserved at -80°C were streaked on 2216E seawater solid medium, cultivated at 25°C-30°C, picked a single colony and inoculated in 2216E seawater liquid medium shake flasks and cultured for 6-8 hours to obtain seed liquid. The obtained seeds The liquid was transferred to fresh 2216E seawater liquid medium at a ratio of 1:100 by volume, fermented and cultured at 25-30°C and 120-200rpm for 24-36 hours to obtain a fermented liquid, and the obtained fermented liquid was centrifuged at 5000g for 30 minutes, harvested The bacteria slime is dried at a constant temperature of 60°C to a constant weight, and the obtained bacteria and corn starch are mixed in a weight ratio of 1:5 to make a dry bacteria powder. The number of M001 viable bacteria per gram of dry bacteria powder is not less than 1012CFU.

[0041] The composition of the 2216E seawater medium is, by mass percentage: 0.5% pepto...

Embodiment 2

[0043] Application of dry bacterial powder of Bacillus amyloliquefaciens M001:

[0044] Step 1) feed preparation. Add the dry bacteria powder of Bacillus amyloliquefaciens M001 to the fish basic feed (according to the mass percentage: fish meal 40%, peanut meal 10%, soybean meal 10%, rapeseed meal 8.4%, flour 26%, fish oil 2%, Premix 2%), formulated to contain live Bacillus amyloliquefaciens M001 in 10 per gram of feed 8 -109 CFU’s probiotic feed, with egg white as a binder, mixed with an appropriate amount of water, extruded with a small meat grinder to make pellet feed, dried at 60°C until the moisture content is less than 10%, packaged in separate bags and stored at 4°C spare.

[0045] Step 2) Turbot farming. 180 healthy turbot with an average body weight of 40.7g were temporarily raised in the recirculating aquaculture system for 2 weeks and then randomly divided into two treatment groups, the experimental group and the control group. Each treatment group had 3 parallel...

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Abstract

The invention disclosesbacillus amyloliquefaciens M001 and an application thereof. The bacillus amyloliquefaciens M001 is preserved in the CGMCC(China General Microbiological Culture Collection Center)of CCCCM(China Committee for Culture Collection of Microorganisms), the preservation date is Nov. 4th, 2013, and the preservation number is CGMCC No.8433. Thestrain is isolated and screened from the intestinal tract of paralichthysolivaceus, and can obviously improve the nonspecific immune level of cultured fish and enhance the capacity of resisting bacterial pathogen infection of the cultured fish.

Description

technical field [0001] The invention relates to the technical field of aquatic microorganism application, in particular to a Bacillus amyloliquefaciens and its application. Background technique [0002] my country's aquaculture output has ranked first in the world for more than ten consecutive years, and it has become one of the fastest-growing industries in large-scale agriculture. Due to the intensive and rapid development of the aquaculture industry for many years, coupled with the continuous deterioration of the aquaculture environment and the decline in the stress resistance of the aquaculture species, it eventually led to frequent occurrence of aquaculture animal diseases and directly caused huge economic losses. Diseases have become a bottleneck restricting the sustainable and healthy development of the aquaculture industry. The prevention and control of aquatic diseases in my country mainly relies on chemical drugs such as antibiotics. Due to the adverse effects of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/07A23K10/18A23K20/163A23K50/80
CPCY02A40/818
Inventor 莫照兰李贵阳黄倢李杰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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