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A simple and rapid method for detecting l-cysteine

A cysteine, rapid technology, applied in color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, analysis by making materials undergo chemical reactions, etc., can solve the problem of high detection limit, selection Problems such as poor performance and low sensitivity

Active Publication Date: 2016-12-28
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quantitative determination of L-cysteine ​​is generally carried out by utilizing its reducibility and its reaction with some organic reagents, and then using electrochemical, chemiluminescence, catalytic kinetics and fluorescence methods. These methods are complicated to operate. , The sensitivity is not high or the selectivity is poor, and some of the above methods require large instruments, some require professionals, and some have higher detection limits

Method used

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  • A simple and rapid method for detecting l-cysteine
  • A simple and rapid method for detecting l-cysteine
  • A simple and rapid method for detecting l-cysteine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Determination of L-cysteine ​​concentration in pig liver.

[0029] (1) Sample acquisition: take fresh pig liver

[0030] (2) Sample pretreatment: Cut 2g of sample into pieces with tissue scissors, put it into a 10ml vial, then add deionized water to make up the volume to 10ml, and then homogenize with a homogenizer. Add the same volume of ethanol to the homogenate to remove protein and other impurities, mix thoroughly and centrifuge at 10,000 rpm for 10 minutes, and take the supernatant. The supernatant was filtered with a 0.22 μm membrane filter, and the filtrate was diluted 10 times for later use.

[0031] (3) Take 800 μl of the sample to be tested and add 100 μl of solution A.

[0032] (4) Add 15 μl of solution B and 5 μl of solution C to the liquid obtained in step (3), and react for 5 minutes in a boiling water bath.

[0033] (5) Concentration adjustment: If the color of the reaction solution obtained in step (4) is too yellow, the sample to be tested...

Embodiment 2

[0039] Example 2: Determination of the concentration of L-cysteine ​​in pig liver by standard addition.

[0040] (1) Obtaining of samples: take fresh pig liver.

[0041] (2) Sample pretreatment: Cut 2g of sample into pieces with tissue scissors, put it into a 10ml vial, then add deionized water to make up the volume to 10ml, and then homogenize with a homogenizer. Add an equal volume of ethanol to the homogenate to remove protein and other impurities, mix thoroughly and centrifuge at 10,000 rpm for 10 minutes, and take the supernatant. The supernatant was filtered with a 0.22 μm filter membrane, the filtrate was diluted 10 times, and 5 μM standard L-cysteine ​​was added for use.

[0042] (3) Take 800ml of the sample to be tested and add 100μl of solution A.

[0043] (4) Add 15 μl of solution B and 5 μl of solution C to the liquid obtained in step (3), and react for 5 minutes in a boiling water bath.

[0044] (5) Concentration adjustment: If the color of the reaction solutio...

Embodiment 3

[0049] Implementation Example 3: Concentration spike determination of L-cysteine ​​in pig liver.

[0050] (1) Obtaining of samples: take fresh pig liver.

[0051] (2) Sample pretreatment: Cut 2g of sample into pieces with tissue scissors, put it into a 10ml vial, then add deionized water to make up the volume to 10ml, and then homogenize with a homogenizer. Add an equal volume of ethanol to the homogenate to remove protein and other impurities, centrifuge at 10,000 rpm after thorough mixing, and take the supernatant. The supernatant was filtered with a 0.22 μm membrane filter, the filtrate was diluted 10 times, and 10 μM standard L-cysteine ​​was added for use.

[0052] (3) Take 800ml of the sample to be tested and add 100μl of solution A.

[0053] (4) Add 15 μl of solution B and 5 μl of solution C to the liquid obtained in step (3), and react for 5 minutes in a boiling water bath.

[0054] (5) Concentration adjustment: If the color of the reaction solution obtained in step...

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Abstract

The invention discloses an ultraviolet colorimetric method for simply rapidly detecting L-cysteine. The method is based on a reaction that L-cysteine and 1-chloro-2,4-dinitrobenzene are reacted in boiling water bath to rapidly generate a yellow complex, and also hexadecyl trimethyl ammonium bromide is capable of accelerating the reaction efficiency. 20 mu M of L-cysteine in the reaction can be detected by naked eyes. The detection limit by employing an ultraviolet detector is 0.5 mu M. The method has the advantages of being simple and convenient in operation, short in consume time, rapid, specific, visual by naked eyes, sensitive, economic and the like.

Description

technical field [0001] The invention relates to the detection field of the color reaction of organic materials, in particular to a simple and fast method for detecting L-cysteine. Background technique [0002] L-cysteine ​​is a thiol-containing amino acid and one of the important physiologically active substances. As the substrate of reduced glutathione, it can increase the production of glutathione, thereby protecting the stability of the cell membrane and reducing the damage of myocardial cells in ischemia-reperfusion. L-cysteine ​​participates in the reduction of cells and the phospholipid metabolism in the liver, and has the functions of protecting liver cells and promoting liver function. In addition, L-cysteine ​​also stimulates the differentiation of pre-T lymphocytes into mature lymphocytes and increases the body's resistance to certain toxins. Changes in the content of cysteine ​​in organisms and metabolic disorders can lead to the occurrence of some diseases. By ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/33G01N21/31
Inventor 陈令新殷堃张卫卫
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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