Soybean trigonelline synthase as well as coding gene and application thereof

A technology that encodes genes and trigonelline, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of slow research progress and achieve the effect of wide application prospects
CN104031897AInactive Publication Date: 2014-09-10INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Publication Date
2014-09-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a soybean trigonelline synthase (GmTS) as well as a coding gene and application thereof. The protein serving as a substrate of the trigonelline synthase has high specificity; Na<+>, K<+>, NH4<+>, Mg<2+>, Mn<2+> and Ca<2+> do not have significant influence on the enzyme activity; heavy metal ions such as Zn<2+>, Cu<2+>, Fe<2+> and Fe<3+> can significantly inhibit the enzyme activity; when the pH value of the protein is 6.0-7.0, the enzyme activity of the trigonelline synthase is relatively high. The protein is the one of the following (1) or (2): (1) protein shown by SEQ ID No. 6; and (2) proteins which are obtained by substituting and / or deleting and / or adding one or a few amino acid residues to an amino acid sequence shown by SEQ ID No. 6 and have the same function. The trigonelline synthase and the coding gene thereof have wide application prospect in the fields of soybean molecular assisted breeding, human health care and the like.
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Description

technical field

[0001] The invention relates to a trigonelline synthetase, its encoding gene and application, in particular to a soybean trigonelline synthetase, its encoding gene and application, and belongs to the field of biotechnology. Background technique

[0002] Trigonelline is a product of N-methylation of Nicotinate (NA), which belongs to alkaloid compounds. Previous isotopic tracers have shown (mainly using 14 C-labeled nicotinamide, i.e. 14 C-NIM), trigonelline is widely present in higher plants, especially in the seeds of legumes, and in addition, the accumulation of trigonelline is also found in the seeds of crops such as rye, lettuce, sunflower and tomato. Studies have shown that in the cotyledons and hypocotyls of clover and alfalfa (legumes) 14 The main products of C-NIM feeding were trigonelline and part of the metabolites of the compensatory synthesis pathway of Nicotinamide adenine dinucleotide (NAD), and there were no other types of nicotinic acid deri...

Claims

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