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Preparation method and antitumor application of pendulone

A technology for yatenone and yatuenone is applied in the field of preparation of plant extracts, which can solve the problems of unsuitability for industrial preparation of pendant yatenone, low sample yield, small preparation amount, etc., so as to improve bioavailability The effect of high degree of preparation, large amount of preparation and low pollution

Inactive Publication Date: 2014-10-01
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complicated operation, small amount of preparation, and low sample yield, it is not suitable for the industrial preparation of drooping jasperone

Method used

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  • Preparation method and antitumor application of pendulone

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Effect test

Embodiment 1

[0015] The preparation of drooping tweenone

[0016] Get the cane of Tachibana japonica and crush it, weigh 20kg, use 8 times of 90% methanol solution for ultrasonic extraction for 30 minutes, extract 3 times, add the extract to HZ816 macroporous resin column for adsorption after decompression and concentration, and use 5 times of column volume 50 % methanol solution for elution, and then 8 times column volume for 85% methanol solution for elution, the eluent was concentrated to adjust pH4, ethyl acetate was added to stir thoroughly, and the ethyl acetate layer was collected to obtain a solid crude extract. Take chloroform, methanol, and water and mix them according to 3:2:1. After fully layering, take the upper phase and fill the high-speed countercurrent chromatography tube as the stationary phase, turn on the host at a speed of 800 rpm, and pump the lower phase into the flow phase at the same time. After the dynamic balance is established, the flow rate is adjusted to 2ml / m...

Embodiment 2

[0018] Anti-tumor application of drooping jigtenone

[0019] 1. Materials

[0020] Animals: Kunming mice, male and female, weighing 20±2g, provided by Nanjing University of Traditional Chinese Medicine.

[0021] Cell line: Sarcoma 180 ascites-type tumor line (S180), provided by Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

[0022] 2. Method

[0023] Take 50 mice and inoculate each mouse with S 180 The next day, the tumor fluid was randomly divided into 5 groups, 10 rats in each group, which were normal saline control group (NS group), positive control group, low-dose katydine group (1mg / kg), middle-dose group (2mg / kg). kg), high dose group (4mg / kg). 24 hours after the inoculation, the drooping tweedenone administration group was intragastrically administered for 7 consecutive days, and the positive control group was intraperitoneally injected twice during the test, and administered every other day. During this period, the general activities, fur and ...

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Abstract

The invention discloses a preparation method and application of a plant extract and particularly relates to a preparation method and antitumor application of pendulone. The preparation method comprises the following steps of smashing rattans of millettia dielsiana, adding 5-10 times of 50-99% methanol solution, carrying out ultrasonic extraction 2-3 times, and adsorbing in a macroporous resin column after extracting and concentrating; eluting by using 3-9 times of 10-50% methanol solution, and then, eluting by using 5-10 times of 60-90% methanol solution; concentrating the eluate, and regulating the pH value of the solution to 3-5; adding ethyl acetate to sufficiently stir, collecting an ethyl acetate layer, and concentrating to obtain a solid; separating the solid by using a high-speed countercurrent chromatograph, collecting the fraction, and carrying out vacuum drying to obtain pendulone. The preparation method disclosed by the invention is simple and convenient is process operation, high in yield, little in pollution and suitable for producing high-purity pendulone. Pendulone has an antitumor promotion effect; an intestinal absorption enhancer is added in the formula of an oral liquid of pendulone, so that the bioavailability of pendulone is favorably increased.

Description

technical field [0001] The invention discloses a preparation method and application of a plant extract, and in particular relates to a preparation method and anti-tumor application of drooping thuitenone. Background technique [0002] Pendulone is an isoflavone compound, CAS No. 69359-09-7, molecular formula C17H16O6, molecular weight 316.31, molecular structural formula: [0003] [0004] Drooping jaggery ketone is derived from the cane of Millettia dielsiana Harms ex Diels of the leguminosae family. It has anti-tumor promotion effect, significantly inhibits chemical carcinogenesis, and its activity is higher than that of glycyrrhetinic acid. [0005] Through literature search, most of the existing isoflavone active substances are extracted by using silica gel and gel chromatography for ethanol extraction and reflux extraction, silica gel and gel chromatography for separation and other steps. Due to the complicated operation, small amount of preparation and low sample y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/58A61K31/353A61P35/00
CPCC07D311/58A61K31/353
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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