Fermentation culture medium for meleumycin

A fermentation medium, melelamycin technology, applied in the direction of fermentation, microbe-based methods, microbes, etc., can solve the problem of low ratio, achieve the effect of reducing risk and increasing content

Inactive Publication Date: 2014-10-22
CHONGQING DAXIN PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, in scientific research and actual production, the proportion of midecamycin A1 and kitamycin A6 produced by the fermentation of some mebelamycin-producing bacteria is relatively low, so that the quality of the final product is just on the edge of the qualified line, and the unqualified more risky

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Slant and seed culture: the slant was propagated by Streptomyces mika, a bacterium producing mebamycin, and the culture medium of the slant was Gaoshi No. 1 medium at a temperature of 28°C for 5 days. Insert mature slanted seeds into the seed bottle for cultivation at 28°C, shaker speed 220rpm, shaker bottle filling volume 100ml / 750ml, and cultivate for 30 hours. The used seed medium is: 1-2% of glucose, 1-3% of starch, 2-4% of soybean powder, 0.01-0.05% of potassium dihydrogen phosphate, 0.2-0.5% of ammonium sulfate and 0.1-0.3% of calcium carbonate. Fermentation culture: Put the mature seed liquid into the fermentation bottle for cultivation. Inoculum volume 2ml / 20ml, 28°C, shaking table speed 220rpm, shaking flask filling volume 20ml / 250ml, ferment for 48h. The fermentation medium used is: oil 1-10%, glucose 1.5-3.0%, starch 1.5-3.0%, soybean powder 2-5%, yeast powder 0.1-0.3%, fish meal 0.1-0.5%, potassium dihydrogen phosphate 0.05- 0.2%, magnesium sulfate 0.05-0....

Embodiment 2

[0022] Add an emulsifier containing Tween 20 and Tween 80 in a ratio of 1:1 to the oil of the fermentation medium in advance (according to the total amount of the fermentation medium, add 0.2g per 100ml of fermentation medium) and stir well before adding to the culture medium. base. All the other are the same as embodiment one.

[0023] After the fermentation is finished, it is measured that the weight ratio of the mebemycin A1 is 63.78%, and the weight ratio of the mebemycin A6 is 22.68%. The content of mebelamycin produced was 1.17 times that of the control group without emulsifier added.

Embodiment 3

[0025] Add an emulsifier containing Tween 20 and Tween 80 in a ratio of 1:1 to the oil of the fermentation medium in advance (according to the total amount of the fermentation medium, add 0.5g per 100ml of fermentation medium) and stir well before adding to the culture medium. base. All the other are with embodiment 1.

[0026] After the fermentation, it is measured that the weight of mebemycin A1 accounts for 62.14% of the total proportion, and the weight of mebelamycin A6 accounts for 23.58% of the total proportion. The content of mebelamycin produced was 1.11 times that of the control group without emulsifier added.

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Abstract

The invention discloses a fermentation culture medium for meleumycin, and belongs to the production field of meleumycin. The fermentation culture medium comprises the following components: 1.5-3.0% of glucose, 1.5-3.0% of starch, 2-5% of soybean meal, 0.1-0.3% of yeast powder, 0.1-0.5% of fish meal, 0.05-0.2% of potassium dihydrogen phosphate, 0.05-0.2% of magnesium sulfate, 0.1-0.35% of calcium carbonate and 1-10% of oil. The fermentation culture medium also comprises an emulsifying agent. The emulsifying agent may be composed of Tween 20 and Tween 80. A weight ratio of Tween 20 to Tween 80 is 1:1. During addition, the emulsifying agent is firstly added into the oil and mixed uniformly, and then is mixed with other components. The addition of the emulsifying agent can increase content of meleumycin after fermentation and increase a proportion of medemycin A and kitasamycin A6 in all the components, reduces influences of relevant substances, and enables a final product to reach and be better than requirements of pharmacopeia.

Description

technical field [0001] The invention relates to the field of melelamycin production, in particular to a fermentation medium for producing meleomycin. Background technique [0002] Meleumycin (Meleumycin, MLM) is a Streptomyces (S. mycarofaciens10204 and 1748) isolated from the soil of Sichuan and Guangdong by antibiotic workers in my country, and obtained through fermentation and extraction. Mebemycin has good antibacterial effect on erythromycin-sensitive bacteria in Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes and pneumococcus, and other common clinical symptoms such as Streptococcus parvum and Neisseria gonorrhoeae Pathogenic bacteria have certain antibacterial activity. [0003] Oil is one of the important raw materials for the fermentative production of mebamycin. Meleomycin-producing bacteria can produce lipase compatible with oil, decompose fat into glycerol and fatty acids, the former is oxidized to pyruvate, and the latter is oxidized ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/465
Inventor 吴峰谢云张洪兰张葵雷川洋
Owner CHONGQING DAXIN PHARMA
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