Enzyme preparation method of sea collagen peptide capable of promoting wound healing

A technology of marine collagen and collagen peptides, which is applied to the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of lack, unreasonable use of ingredients, waste of processing by-products, etc., to achieve Improve the competitiveness of enterprises, promote healthy and sustainable development, and increase the effect of added value

Active Publication Date: 2014-10-22
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for enzymatically preparing marine collagen peptides with wound healing activity, which is to prepare highly active marine collagen peptides with wound healing effects from sea bass processing by-products by enzymatic method combined with membrane separation technology. Bioactive peptides, which can solve the problems of unreasonable utilization of waste by-products of existing sea bass processing and lack of ingredients for clinical nutritional products for trauma patients

Method used

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  • Enzyme preparation method of sea collagen peptide capable of promoting wound healing
  • Enzyme preparation method of sea collagen peptide capable of promoting wound healing
  • Enzyme preparation method of sea collagen peptide capable of promoting wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Wash and mince the skin and bones of 1kg sea bass, soak in 15L of 0.1mol / L sodium hydroxide solution for 15h to remove impurities; wash with distilled water until neutral, then use 15L of 0.6 Soak in mol / L hydrochloric acid for 15 hours to decalcify; wash with distilled water until neutral, and finally soak in 2L of 10% ether for 24 hours, repeat twice, and degrease for a total of 48 hours;

[0057] (2) After the degreasing is completed, wash repeatedly with distilled water, drain, soak in 1L of 0.5mol / L acetic acid solution for 3 days, centrifuge at 4000r / min for 20min, take the supernatant, and freeze-dry to obtain 200g of collagen;

[0058] (3) Add 2L of distilled water and 4.6g of alkaline protease to 200g of collagen, and enzymolyze it for 3.0h at a temperature of 45°C and a pH value of 9.0, and the extraction rate of collagen peptides is 48%;

[0059] (4) Heat in a water bath at 90°C for 10 minutes to inactivate the enzyme;

[0060] (5) Add 10g of activated c...

Embodiment 2

[0067] (1) Wash and mince the fish head and fish bones of 300g sea bass, soak them in 6L of 0.1mol / L sodium hydroxide solution for 30h to remove impurities; Soak in mol / L hydrochloric acid for 30 hours to decalcify; wash with distilled water until neutral, and finally soak in 0.9L of 10% ether for 24 hours, repeat twice, and degrease for a total of 48 hours;

[0068] (2) After the degreasing is completed, wash with distilled water, drain, soak in 0.3L of 0.5mol / L acetic acid solution for 3 days, centrifuge at 4500r / min for 15min, take the supernatant, and freeze-dry to obtain 30g of collagen;

[0069] (3) Add 0.3L of distilled water and 0.9g of alkaline protease to 30g of collagen, and enzymolyze it for 4.0h at a temperature of 50°C and a pH value of 9.5, and the extraction rate of collagen peptides is 42%;

[0070] (4) Heat in a water bath at 95°C for 15 minutes to inactivate the enzyme;

[0071] (5) Add 1.8g of activated carbon and heat in a water bath at 45°C for 50 minute...

experiment example 3

[0079] (1) Wash and mince the skin and bones of 1kg sea bass, soak in 20L of 0.1mol / L sodium hydroxide solution for 24 hours to remove impurities; wash with distilled water until neutral, then use 20L of 0.6 Soak in mol / L hydrochloric acid for 24 hours to decalcify; wash with distilled water until neutral, and finally soak in 20L of 10% ether for 24 hours, repeat twice, and degrease for a total of 48 hours;

[0080] (2) After the degreasing is completed, wash repeatedly with distilled water, drain, soak in 1L of 0.5mol / L acetic acid solution for 2 days, centrifuge at 4400r / min for 15min, take the supernatant, and freeze-dry to obtain 200g of collagen;

[0081] (3) Add 2L of distilled water and 4.6g of alkaline protease to 200g of collagen, and enzymolyze it for 2.0h at a temperature of 45°C and a pH value of 9.0, and the extraction rate of collagen peptides is 45%;

[0082] (4) Heat in a water bath at 95°C for 10 minutes to inactivate the enzyme;

[0083] (5) Add 12g of activ...

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Abstract

The invention discloses an enzyme preparation method of sea collagen peptide capable of promoting the wound healing. The method comprises the following steps: (1) removing other proteins and impurities from by-products of orange rock-fish processing, opening the three-strand helical structure of collagen; (2) adding acid to extract the pre-treated collagen from the by-products of orange rock-fish processing, freeze-drying the obtained collagen, and saving the freeze-dried collagen as the substrate to prepare collagen peptide through enzymolysis in the next step; (3) adding water into the freeze-dried collagen to prepare a collagen solution, adding protease into the collagen solution to carry out enzymolysis so as to convert collagen into collagen peptide; (4) deactivating the enzyme, cooling; (5) removing the fishy smelling, decoloring; (6) subjecting the collagen peptide to centrifugation, saving the supernate for later use; (7) carrying out membrane separation to collect the collagen peptides with a molecular weight of 1000 to 3000 Da; (8) condensing the separated collagen peptides through rotation evaporation; (9) freeze-drying the condensed collagen peptide into powder. High bioactivity biological active peptide capable of healing the wounds is prepared from by-products of orange rock-fish processing through an enzyme method assisted by a membrane separation technology.

Description

technical field [0001] The invention relates to a preparation method of biologically active peptides, in particular to a method for enzymatically preparing marine collagen peptides with the activity of promoting wound healing. Background technique [0002] Sea bass (scientific name Lateolabrax japonicus ), also known as seven-star perch and perch, is a genus of ray-finned perciformes and perch family. It is distributed in the Western Pacific Ocean and is the largest marine economic fish in Guangdong Province. During the processing of sea bass, a large number of by-products such as fish heads, fish bones and fish skins are produced, which are usually discarded or processed into animal feed, which not only pollutes the environment but also wastes resources. Nagai et al. found that the collagen content in the skin, bone and fin of sea bass was 51.4%, 40.7% and 41.6% (on a dry basis), respectively. Due to its relatively stable triple-helix structure, collagen has a low absorpt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14A61P17/02
Inventor 刘磊张名位魏振承张瑞芬邓媛元唐小俊池建伟李健雄
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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