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Preparation method of water soluble active collagen and epidermis repair growth factor liquid

A growth factor and epidermal repair technology, applied in the fields of medicine and biology, can solve the problems of staying and the bottleneck of large-scale production and application, and achieve the effect of reducing wrinkles, avoiding immune reaction of foreign proteins, and lightening pigmentation.

Inactive Publication Date: 2014-10-29
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current extraction and preparation methods of human placental collagen still remain at the technical level of preparing acid-soluble collagen. Taking patent 201310448097.8 as an example, the human placental collagen described in this patent belongs to acid-soluble collagen. Acidic substances must be used to dissolve collagen. Most acidic substances have a pungent smell and skin irritation. Therefore, acid-soluble collagen is only a crude product of collagen, and there is still a bottleneck before large-scale production and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 (preparation of water-soluble active collagen):

[0040] (1) Extraction of acid-soluble natural collagen: The frozen healthy human placenta was taken and thawed completely at 4°C for 24 hours. Take 100 parts by weight of healthy human placenta, wash it with deionized water until it is flesh-colored. Add 1 weight part of ethylene glycol ethyl ether for degreasing treatment for 3 hours, and then rinse with deionized water for 5 minutes. The above degreasing treatment steps are repeated 3 times. After the treatment, soak in 5 L of NaCl with a concentration of 0.5 mol / L for 24 hours at 4°C to remove residual serum and fat. After soaking, wash with deionized water, and use a meat grinder to grind the placenta fine section at 4°C. Add the ground placenta into a stirring container filled with 0.5mol / L NaCl (5L / Kg placenta), use a stirrer to stir at 100rpm at 4°C for 8 hours to mix evenly, and then centrifuge at 10,000rpm at 4°C for 30 minutes , take the precipi...

Embodiment 2

[0044] Embodiment 2 (preparation of water-soluble active collagen):

[0045] (1) Extraction of acid-soluble natural collagen: The frozen healthy human placenta was taken and thawed completely at 6° C. for 12 hours. Take 100 parts by weight of healthy human placenta, wash it with deionized water until it is flesh-colored. Add 2 parts by weight of fatty acid polyoxyethylene ether for degreasing treatment for 2 hours, then rinse with deionized water for 10 minutes, and repeat the above degreasing treatment step once. After the treatment, soak in 5 L of NaCl with a concentration of 5 mol / L for 24 hours at 6°C to remove residual serum and fat. After soaking, wash with deionized water, and use a meat grinder to grind the placenta fine section at 10°C. Add the ground placenta into a stirring container filled with 5mol / L NaCl (5L / Kg placenta), use a stirrer to mix well at 10°C at 50rpm for 10 hours, and then place in 10 Centrifuge at 13000rpm for 10 minutes at 10°C, take the precip...

Embodiment 3

[0049] Embodiment 3 (preparation of water-soluble active collagen):

[0050] (1) Extraction of acid-soluble natural collagen: Take the frozen healthy human placenta and put it in the environment of 2° C. for 48 hours to thaw completely. Take 100 parts by weight of healthy human placenta, wash it with deionized water until it is flesh-colored. After adding 1.5 parts by weight of alkylphenol polyoxyethylene ether for degreasing treatment for 2.5 hours, it was rinsed with deionized water for 10 minutes. After the treatment, soak in 5 L of NaCl with a concentration of 5 mol / L for 24 hours at 2°C to remove residual serum and fat. After soaking, wash with deionized water, and use a meat grinder to grind the thin section of placenta at 2°C. Add the ground placenta into a stirring container filled with 2mol / L NaCl (5L / Kg placenta), use a stirrer to mix well at 80rpm at 2°C for 12 hours, and then centrifuge at 10,000rpm at 2°C for 60 minutes. Take the precipitate and add it to deion...

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PUM

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Abstract

The invention discloses a preparation method of water soluble active collagen. The preparation method comprises the following steps: extraction of acid-soluble natural collagen; purification of acid-soluble natural collagen; preparation of a water soluble active collagen coarse product; and preparation of the water soluble active collagen. The invention further discloses an epidermis repair growth factor liquid containing the water soluble active collagen prepared by the preparation method. According to the technical field of the water soluble active collagen disclosed by the invention, wastes are turned to wealth, so that the value of umbilical cord and placenta is utilized and developed to the maximum extent. The water soluble active collagen is extracted from a healthy human placenta and / or umbilical cord, is easy to absorb and avoids immunoreaction caused by allogenic materials. The water soluble active collagen is free from external factors and does not contain chemical additives, so that the water soluble active collagen is closer to nature when in use, and the combined effect is best. By taking the water soluble active collagen serving as a main raw material can be matched with various cell factors and plant essences, so as to achieve the effects of repairing and regenerating skin, preventing sensitivity, diminishing inflammation, reducing wrinkles and lightening stains, avoiding adverse reaction and achieving good safety.

Description

technical field [0001] The invention belongs to the technical fields of biology and medicine, and relates to a preparation method of water-soluble active collagen and an epidermal repair growth factor solution containing the water-soluble active collagen. Background technique [0002] The root of skin wound repair lies in the regeneration ability of cells at the wound site. Cytokines and extracellular matrix provide rich nutrition and regeneration power for cell regeneration, and are the source of stimulating cell regeneration. Most of the existing epidermal repair growth factor solutions use large molecular weight sodium hyaluronate as a structural support or slow-release material, and then add various cytokine components and antiseptic stabilizers. When this type of repair factor liquid is used for epidermal repair, since the skin cannot effectively absorb and decompose the macromolecular sodium hyaluronate, even if it contains multiple cytokine components, it still canno...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78A61K38/39A61K8/97A61K8/65A61Q19/00A61P17/02
Inventor 胡忠国廖知恒陈静娴海泉夷慧群赵峻
Owner CHENGDU QINGKE BIOTECH
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