Rapid mycorrhization method of orchid aseptic seedlings

A sterile seedling and mycorrhizal technology, applied in the agricultural field, can solve the problems of death, slow growth of plants, entanglement of plants, etc., and achieve the effects of high infection rate, high quality of seedling growth and fast effect.

Active Publication Date: 2014-11-05
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary medium is generally more conducive to the growth of fungi, and it is prone to overgrowth and entanglement of plants due to excess nutrition of fungi, showing pathogenicity to plants, resulting in slow growth or even death of plants

Method used

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  • Rapid mycorrhization method of orchid aseptic seedlings
  • Rapid mycorrhization method of orchid aseptic seedlings
  • Rapid mycorrhization method of orchid aseptic seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and identification of orchid mycorrhizal fungi:

[0021] 1. Separation of root materials: Take the potted Chunlan from Shanghai Academy of Agricultural Sciences and move it to a shaded greenhouse for later use.

[0022] 2. Separation and identification medium:

[0023] Use malt extract medium (MEA), the formula is: malt extract 20g, tryptone 1g, glucose 20g, agar 20g, distilled water to 1000ml, then autoclave at 121°C for 20 minutes, cool to 60 When the temperature is below 0.03 g streptomycin is added.

[0024] 3. Separation and morphological identification methods:

[0025] Isolation of mycorrhizal fungal strains: take several sections of roots of fresh and healthy orchid plants, rinse them under tap water until there is no sludge on the surface of the roots. Cut the roots into several small sections about 10cm long, wash them three times with sterile water, soak the filter paper in 75% alcohol for 60s, then immerse in 2% sodium hypochlorite for...

Embodiment 2

[0040] 1. Orchid tissue culture seedling materials: collect tissue culture seedlings of orchid "Red Beauty", the selected tissue culture seedlings are basically the same, with a fresh weight of about 0.6g and a plant height of about 8cm.

[0041] 2. Cultivation substrate:

[0042] Select fully sterilized and dried peat: yellow sand mixed in a certain proportion as the cultivation substrate, the specific formula is peat: yellow sand 1:2, pour MMN liquid medium, 500mL / 1500g cultivation substrate, mix evenly and pack to 640mL cultivation flat, after autoclaving, cool to room temperature. Under aseptic conditions, 3 orchid seedlings were transplanted in each bottle, and 6 bottles of orchid seedlings were inoculated in each treatment as a repetition, and placed in the tissue culture room for sterile culture. After one week of growth, check for contamination, and inoculate the strains to be tested in non-polluted tissue culture bottles, that is, place a 0.5 cm diameter bacterial ca...

Embodiment 3

[0047] Materials for orchid tissue culture seedlings: The tissue culture seedlings of the orchid "Red Beauty" were collected, and the selected tissue culture seedlings were basically the same, with a fresh weight of about 0.9g and a plant height of about 10cm. The test is provided with 2 kinds of inoculation conditions, one adopts the method of the present invention, that is, the cultivation substrate and the inoculation treatment method are the same as the implementation case 3; the other uses DE medium (prescription: 10 grams of glucose, 5 grams of tryptone, dihydrogen phosphate Potassium 1 g, magnesium sulfate 0.5 g, Bengal red 0.033 g, agar 20 g, distilled water to 1000 ml, adjust the pH value to 5.0, then autoclave at 121°C for 20 minutes, cool down to below 60°C and pour the plates for use ), the inoculation method is similar to the method of the present invention. In this case, the strain LH53 was inoculated, and the seedling growth and infection were counted after 80 d...

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Abstract

The invention relates to a rapid mycorrhization method of orchid aseptic seedlings. By the method, problems of slow seedling growth, high mortality and the like in artificial breeding of orchid in the prior art are solved. The rapid mycorrhization method of orchid aseptic seedlings comprises a step of preparing a culture medium and a nutrient solution and a step of inoculating aseptic seedlings, wherein the culture medium is composed of peat and sand; the volume ratio of peat to sand is 1:1.5-3; and during the step of inoculating aseptic seedlings, tissue culture rooting seedlings or aseptic sowing seedlings are transplanted into the culture medium which has undergone aseptic treatment, and a strain piece is inoculated near roots of the seedlings. The strain piece is a strain with the collection number of CGMCC No: 9222 or CGMCC No: 9247. The strain piece inoculation position is 0.3-2cm from the roots. The seedlings are placed in a culture room to be cultured after inoculation. By the above method, orchid seedlings form mycorrhiza rapidly, infection rate is high, and seedlings grow fast. The method can be used in orchid seedling production.

Description

Technical field: [0001] The invention relates to the field of agriculture, in particular to a cultivation technique for orchids, in particular to a method for rapid mycorrhization of orchid sterile seedlings. Background technique: [0002] Orchidaceae, referred to as orchids, is a class of precious flowers with important ornamental and economic value. Tissue culture is often used to propagate sterile seedlings, but there are difficulties in tissue culture, small multiplication coefficient, slow growth, and low survival rate when transplanting. And other issues. Orchid mycorrhizal fungi play an important role in the growth of orchid plants, promote seed germination, promote the absorption and utilization of nutrients such as N and P during the plant growth stage, secrete hormones to promote plant growth, and even antagonize pathogenic bacteria inhibit its growth. Mycorrhizalization of seedlings is an effective method to overcome the slow growth of orchid seedlings and impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N1/14C12R1/645
Inventor 黄芳张春英王强谢军峰杨慧丽魏翔莺
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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