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A method for extracting total anthraquinones in Polygonum cuspidatum by aqueous enzymatic method-semi-bionic method

A semi-biomimetic and water-enzymatic technology, applied in the field of natural product extraction, can solve problems such as low extraction efficiency and waste of medicinal material resources, and achieve the effects of avoiding environmental pollution problems, improving extraction rate, and reducing mass transfer resistance.

Inactive Publication Date: 2016-04-27
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The aqueous enzymatic method is a relatively efficient and environmentally friendly extraction method, but the extraction efficiency is not high, which to a certain extent has caused a waste of medicinal resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting total anthraquinones in Polygonum cuspidatum by an aqueous enzymatic method-semi-bionic method, comprising the following steps:

[0025] 1) Treatment of Polygonum cuspidatum medicinal material: Wash and dry the medicinal material Polygonum cuspidatum, sieve the powder, and dry to constant weight to obtain medicinal material powder of Polygonum cuspidatum;

[0026] 2) Water enzymatic extraction: Accurately weigh 10g of Polygonum cuspidatum medicinal material powder, add hydrolase reaction solution to Polygonum cuspidatum powder at a solid-liquid ratio of 1g: 16mL, after mixing, adjust the pH value to 4.5, under stirring conditions, at 50 ℃ in a water bath for 6 hours, raise the temperature of the water bath to 80°C, heat for 10 minutes, cool and filter; wherein, the hydrolytic enzyme reaction solution is to dissolve the cellulase in the proportion of 125 mg of cellulase per 100 mL of distilled water made in distilled water;

[0027] 3) Extraction...

Embodiment 2

[0039] A method for extracting total anthraquinones in Polygonum cuspidatum by an aqueous enzymatic method-semi-bionic method, comprising the following steps:

[0040] 1) Processing of Polygonum cuspidatum medicinal material: Wash and dry the medicinal material Polygonum cuspidatum, powder and sieve, and dry to constant weight to obtain medicinal material powder of Polygonum cuspidatum;

[0041] 2) Water enzymatic extraction: Accurately weigh 10g of Polygonum cuspidatum medicinal material powder, add hydrolase reaction solution to Polygonum cuspidatum powder at a solid-liquid ratio of 1g:10mL, after mixing, adjust the pH value to 4.5, under stirring conditions, at 50 ℃ in a water bath for 7 hours, raise the temperature of the water bath to 80°C, heat for 10 minutes, cool and filter; wherein, the hydrolytic enzyme reaction solution is to dissolve the cellulase in the proportion of 125 mg of cellulase per 100 mL of distilled water made in distilled water;

[0042]3) Extraction ...

Embodiment 3

[0046] A method for extracting total anthraquinones in Polygonum cuspidatum by an aqueous enzymatic method-semi-bionic method, comprising the following steps:

[0047] 1) Treatment of Polygonum cuspidatum medicinal material: Wash and dry the medicinal material Polygonum cuspidatum, sieve the powder, and dry to constant weight to obtain medicinal material powder of Polygonum cuspidatum;

[0048] 2) Water enzymatic extraction: Accurately weigh 10g of Polygonum cuspidatum medicinal material powder, add hydrolase reaction solution to Polygonum cuspidatum powder at a solid-liquid ratio of 1g:15mL, mix well, adjust the pH value to 4.0, under stirring conditions, at 55 ℃ in a water bath for 6 hours, raise the temperature of the water bath to 80°C, heat for 10 minutes, cool and filter; wherein, for every 100mL of hydrolytic enzyme reaction solution, dissolve the cellulase in the proportion of 125mg of cellulase per 100mL of distilled water made in distilled water;

[0049] 3) Extract...

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PUM

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Abstract

The invention discloses a method for extracting total anthraquinone from from Rhizoma Polygoni Cuspidati through an aqueous enzymatic process-semi-bionic process. The method comprises the following steps: 1, weighing Rhizoma Polygoni Cuspidati powder, adding a hydrolase reaction liquid, adjusting to an appropriate pH value, uniformly stirring, carrying out enzymatic hydrolysis for 4-8h, carrying out high temperature enzyme inactivation, and filtering; 2, adding a citric acid-sodium dihydrogen phosphate buffer solution to the enzymatic hydrolyzed Rhizoma Polygoni Cuspidati in order to adjust the pH value to 1.5-3.0, extracting, and filtering; 3, adding the citric acid-sodium dihydrogen phosphate buffer solution to filter residues in order to adjust the pH value to 6.5-7.5, extracting, and filtering; 4, adding the citric acid-sodium dihydrogen phosphate buffer solution to newly obtained filter residues in order to adjust the pH value to 8.0-8.5, extracting, and filtering; and 5, mixing filtrates obtained in step 1-4, concentrating, and carrying out vacuum drying to obtain an extract containing Rhizoma Polygoni Cuspidati anthraquinone. The method avoids the use of organic solvents; compared with microwave extraction, ultrasonic extraction and other assisted extraction methods, the method disclosed in the invention has the advantages of high extraction rate, simple extraction device and mild extraction conditions; and the method disclosed in the invention is an environmentally-friendly extraction method suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and relates to an extraction method of anthraquinone, in particular to an aqueous enzymatic-semi-bionic method for extracting total anthraquinone in Polygonum cuspidatum. Background technique [0002] Polygonum cuspidatum is the root and rhizome of Polygonum cuspidatum, a plant of the genus Polygonum cuspidatum. It is a traditional Chinese medicine in my country. It is clinically used for diseases such as damp-heat jaundice, lung-heat cough, sore carbuncle, swelling and pain, and traumatic injury. Polygonum cuspidatum mainly contains anthraquinones, stilbenes, flavonoids, and phenolic acids, which have important physiological activities such as antibacterial, antithrombotic, and lipid-lowering. Polygonum cuspidatum anthraquinone is an important antibacterial component. Its extraction is mostly done with organic solvents, which is costly and causes great environmental pollution...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/704A61K47/42A61K47/12
Inventor 张卉郭增军覃源芮李梦怡席可薛璇玑
Owner XI AN JIAOTONG UNIV