Method for preparing walnut polypeptide nano-selenium with anti-tumor activity

An anti-tumor activity, walnut polypeptide technology is applied in the field of preparation of walnut polypeptide nanometer selenium, and achieves the effects of simple preparation process, inhibiting tumor cell proliferation, and obvious inhibitory effect.

Active Publication Date: 2014-12-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In summary, there is no report on the preparation of nan

Method used

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  • Method for preparing walnut polypeptide nano-selenium with anti-tumor activity
  • Method for preparing walnut polypeptide nano-selenium with anti-tumor activity
  • Method for preparing walnut polypeptide nano-selenium with anti-tumor activity

Examples

Experimental program
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Effect test

Embodiment 1

[0040] (1) Restricted enzymatic hydrolysis: mix walnut meal (the protein content of walnut meal is 40%, w / w) with water at a mass ratio of 1:4, and add neutral protease (Nanning, Guangxi) accounting for 2.0% of the protein content of walnut meal Pangbo Bioengineering Co., Ltd.), enzymolysis at 53°C for 30 h; inactivate the enzyme in a boiling water bath for 10 min, centrifuge, take the supernatant, and filter with suction to obtain the walnut polypeptide liquid (the protein recovery rate is 45.2%);

[0041] (2) Concentrate and spray-dry to obtain walnut polypeptide: Concentrate walnut dreg protein enzymatic hydrolyzate at 60°C by vacuum rotary evaporation to a solid content of 20 Brix, then spray-dry to obtain walnut polypeptide, and the obtained powder has uniform fineness.

[0042] (3) Anti-tumor effect of walnut polypeptide: it has significant anti-tumor effect determined by comprehensive indicators such as its influence on tumor cell survival rate and tumor cell morpho...

Embodiment 2

[0044] (1) Restricted enzymatic hydrolysis: mix walnut meal (the protein content of walnut meal is 43%, w / w) and water at a mass ratio of 1:4, and add neutral protease (Nanning, Guangxi, Pang Bo Biological Engineering Co., Ltd.), enzymatic hydrolysis at 53°C for 30 h; inactivate the enzyme in a boiling water bath for 10 min, centrifuge, take the supernatant, and filter with suction to obtain walnut polypeptide liquid (protein recovery rate is 45.2%);

[0045] (2) Concentrate and spray dry to obtain walnut polypeptide: Concentrate the enzymatic hydrolyzate of walnut meal protein at 60°C by vacuum rotary evaporation to a solid content of 20 Brix, then spray dry to obtain walnut polypeptide, and the obtained powder has uniform fineness.

[0046] (3) Add inorganic selenium source and vitamin C to walnut polypeptide solution to prepare functional nano-selenium sol: prepare 8 mmol / l vitamin C solution and 0.3 mmol / l sodium selenite solution, add walnut polypeptide powd...

Embodiment 3

[0051] (1) Restricted enzymatic hydrolysis: mix walnut meal (the protein content of walnut meal is 45%, w / w) and water at a mass ratio of 1:6, and add neutral protease (Nanning, Guangxi, Pang Bo Biological Engineering Co., Ltd.), enzymatic hydrolysis at 55°C for 35 h; inactivate the enzyme in a boiling water bath for 10 min, centrifuge, take the supernatant, and filter with suction to obtain the walnut polypeptide liquid (the protein recovery rate is 50.08%);

[0052] (2) Concentrate and spray-dry to obtain walnut polypeptide: vacuum concentrate and separate the product, spray-dry to obtain walnut polypeptide for regulating nano-selenium; concentrate the walnut meal protein hydrolyzate at 65°C by vacuum rotary evaporation to a solid of 25 Brix Finally, the walnut polypeptide is obtained by spray drying, and the particle size of the obtained powder is uniform.

[0053] (3) Adding inorganic selenium source and vitamin C to walnut polypeptide solution to prepare functio...

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Abstract

The invention discloses a method for preparing walnut polypeptide nano-selenium with anti-tumor activity. The method comprises the following steps: (1) performing limited enzymatic hydrolysis on walnut dreg; (2) concentrating, and spray-drying to obtain walnut polypeptide; (3) adding an inorganic selenium source and vitamin C into the walnut polypeptide solution to prepare functional nano-selenium sol; (4) dialyzing the sol; and (5) carrying out freeze drying to obtain the walnut polypeptide nano-selenium. The dissolved walnut polypeptide nano-selenium is orange sol, and the morphology of the polypeptide nano-selenium is observed by a scanning electron microscope, and nano-particles with uniform particle size can be observed. The walnut polypeptide nano-selenium disclosed by the invention can be used for preparing selenium supplement agent healthy products and medicines. Cell experiments prove that the sol has a remarkable anti-tumor bioactivity, and is expected to become a high-efficiency low-toxicity preparation for treating cancers, in particular breast cancer.

Description

technical field [0001] The invention relates to a preparation method of walnut polypeptide nano-selenium, in particular to a preparation method of walnut polypeptide nano-selenium with antitumor activity. Background technique [0002] Selenium is one of the necessary and indispensable nutritional elements in organisms. Its main biological function is to form glutathione catalase (GSH-Px), which catalyzes the transformation of reduced glutathione (GSH) into Oxidized glutathione (GSSG) converts toxic peroxides into non-toxic hydroxylates (ROH). The only atoms that undergo redox reactions in the GSH-Px molecule are Se, ROOH and H 2 o 2 It is decomposed under the catalysis of GSH-Px, thereby protecting cells and their tissues from being damaged by peroxides, especially protecting the membranes of cells and organelles, such as mitochondria, microsomes, and lysosomes. Selenium and its compounds play an important role in anti-oxidation, anti-tumor, and improving immunity. Insuf...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61P35/00A61K33/04
Inventor 任娇艳张榕廖文镇林泽华刘鹏
Owner SOUTH CHINA UNIV OF TECH
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