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Beta-tricalcium phosphate/alginic acid composite microsphere and preparation method and application of beta-tricalcium phosphate/alginic acid composite microsphere

A technology of tricalcium phosphate and composite microspheres, which is applied in the field of biomedical composite materials, can solve the problems that do not involve the method of changing the particle size of the microspheres, cannot fully meet the clinical needs, and do not involve the regulation method of the particle size. Easy to produce in batches, meet clinical needs, and easy to control the process

Active Publication Date: 2014-12-03
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Chinese patent CN100554327C macromolecularly imprinted calcium phosphate / alginic acid hybrid microspheres and its preparation method introduces a method for preparing calcium phosphate / alginic acid microspheres by calcium ion cross-linking, but does not involve particle size regulation methods
[0010] Xu Chongting (Preparation and Performance Evaluation of β-Tricalcium Phosphate / Alginic Acid Composite Sustained-release Microspheres, Master Thesis of Jinan University) introduced the preparation of composite microspheres with β-TCP and sodium alginate as raw materials. The millimeter level cannot fully meet the clinical needs, and the method of changing the particle size of the microspheres is not involved in the paper

Method used

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  • Beta-tricalcium phosphate/alginic acid composite microsphere and preparation method and application of beta-tricalcium phosphate/alginic acid composite microsphere
  • Beta-tricalcium phosphate/alginic acid composite microsphere and preparation method and application of beta-tricalcium phosphate/alginic acid composite microsphere
  • Beta-tricalcium phosphate/alginic acid composite microsphere and preparation method and application of beta-tricalcium phosphate/alginic acid composite microsphere

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Experimental program
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Effect test

Embodiment 1

[0053] A preparation method of β-tricalcium phosphate / alginic acid composite microspheres, comprising the following steps:

[0054] (1) Weigh ALG-Na solid (Tianjin Ruijinte Chemical Co., Ltd., chemically pure) and add it to 20mL deionized water at a ratio of 0.01g / mL, and stir with a stirrer until the ALG-Na solid is completely dissolved , to obtain 0.01g / mL ALG-Na solution;

[0055] Weigh β-TCP solid (SIGMA-ALDRICH, 21218) into the ALG-Na solution at a ratio of 0.2 g / mL, and stir with a stirrer. After mixing evenly, put it in a refrigerator at 4°C to remove air bubbles, and obtain 0.2g / mL β-TCP and 0.01g / mL ALG-Na mixed solution;

[0056] (2) Add the mixed solution obtained in step (1) into a 50mL disposable syringe, use a medical 21G flat-headed needle, connect an electrode on the needle of the syringe, the needle is positive, and the voltage is 6kv;

[0057] Weigh 2.2196g of CaCl 2 (Guangzhou Chemical Reagent Factory, analytically pure) was dissolved in 200mL deionized w...

Embodiment 2

[0065] A preparation method of β-tricalcium phosphate / alginic acid composite microspheres, comprising the following steps:

[0066] (1) Weigh ALG-Na solid (Tianjin Ruijinte Chemical Co., Ltd., chemically pure) and add it to 20mL deionized water at a ratio of 0.01g / mL, and stir with a stirrer until the ALG-Na solid is completely dissolved , to obtain 0.01g / mL ALG-Na solution;

[0067] Weigh β-TCP solid (SIGMA-ALDRICH, 21218) into the ALG-Na solution at a ratio of 0.2 g / mL, and stir with a stirrer. After mixing evenly, put it in a refrigerator at 4°C to remove air bubbles, and obtain 0.2g / mL β-TCP and 0.01g / mL ALG-Na mixed solution;

[0068] (2) Add the mixed solution obtained in step (1) into a 50mL disposable syringe, use a medical 21G flat needle, connect an electrode on the needle of the syringe, the needle is positive, and the voltage is 12kv;

[0069] Weigh 2.2196g of CaCl 2 (Guangzhou Chemical Reagent Factory, analytically pure) was dissolved in 200mL deionized water t...

Embodiment 3

[0076] A preparation method of β-tricalcium phosphate / alginic acid composite microspheres, comprising the following steps:

[0077] (1) Weigh ALG-Na solid (Tianjin Ruijinte Chemical Co., Ltd., chemically pure) and add it to 20mL deionized water at a ratio of 0.01g / mL, and stir with a stirrer until the ALG-Na solid is completely dissolved , to obtain 0.01g / mL ALG-Na solution;

[0078] Weigh β-TCP solid (SIGMA-ALDRICH, 21218) into the ALG-Na solution at a ratio of 0.2 g / mL, and stir with a stirrer. After mixing evenly, put it in a refrigerator at 4°C to remove air bubbles, and obtain 0.2g / mL β-TCP and 0.01g / mL ALG-Na mixed solution;

[0079] (2) Add the mixed solution obtained in step (1) into a 50mL disposable syringe, use a medical 21G flat-headed needle, and connect an electrode on the needle of the syringe, the needle is positive, and the applied voltage is 20kv;

[0080] Weigh 2.2196g of CaCl 2 (Guangzhou Chemical Reagent Factory, analytically pure) was dissolved in 200m...

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Abstract

The invention discloses a beta-tricalcium phosphate / alginic acid composite microsphere and a preparation method and an application of the beta-tricalcium phosphate / alginic acid composite microsphere. The method comprises the following steps: dropwise adding a mixed solution of beta-tricalcium phosphate and sodium alginate into a calcium chloride solution of which the pH value is 4.5 to 5.0 by virtue of an electrostatic liquid dropping device, and continuing to calcify for a period of time after dropwise adding; pouring out the calcium chloride solution after reaction, then washing a calcified crosslinked microsphere with deionized water, and airing, so as to obtain the beta-tricalcium phosphate / alginic acid composite microsphere. The method is carried out at normal temperature without heating or reference to poisonous and harmful liquid and other volatile solids and gases. The beta-tricalcium phosphate / alginic acid composite microsphere prepared by using the preparation method has the advantage of wide particle size adjusting range and can be widely applied to tissue repair and drug carriers, and a plurality of micropores are formed in the surface of the beta-tricalcium phosphate / alginic acid composite microsphere, so as to be easy for cells to adhere, for tissue fluid to permeate and for loading of drugs, growth factors and cells, and therefore, the beta-tricalcium phosphate / alginic acid composite microsphere can be widely applied to tissue repair and drug carriers.

Description

technical field [0001] The invention belongs to the field of biomedical composite materials, and specifically relates to a β-tricalcium phosphate / alginic acid composite microsphere and a preparation method and application thereof. Background technique [0002] β-tricalcium phosphate (β-TCP) is mainly composed of calcium and phosphorus. Its composition is similar to the inorganic composition of bone matrix. It combines well with bone tissue and has good degradability. A large number of experiments have proved that β-TCP has no adverse reaction to bone marrow hematopoietic function, no rejection reaction, no acute toxicity reaction, no carcinogenesis, and no allergic phenomenon. Therefore, β-TCP can be widely used in joint and spinal fusion, extremity trauma, oral and maxillofacial surgery, cardiovascular surgery, and filling periodontal cavities. During treatment, β-TCP is often filled in the form of granules. Regularly shaped spherical particles have good fluidity and are ...

Claims

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Application Information

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IPC IPC(8): B01J13/02A61L27/20A61L27/12A61L27/50A61K47/36A61K47/04A61K9/16
Inventor 符青云李红周长忍
Owner JINAN UNIVERSITY
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