Maca leaf black tea and preparation method thereof

A technology of maca leaves and dried maca leaves, which is applied in the field of black tea preparation and maca leaf black tea preparation, achieves a significant dose-effect relationship, is widely promoted and used, and has the effect of scavenging DPPH free radicals

Active Publication Date: 2014-12-10
乳源瑶族自治县瑶山王茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the development and utilization of maca leaves

Method used

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  • Maca leaf black tea and preparation method thereof
  • Maca leaf black tea and preparation method thereof
  • Maca leaf black tea and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment one: the preparation of maca leaf black tea

[0028] The preparation of maca leaf black tea is prepared by the following specific methods:

[0029] (1) Leaf picking and treatment: pick the middle and upper leaves of maca in late autumn that are free from pollution and pests. Put it in the shade to dry in the room until there is no water drop, stack the maca leaves with petioles removed into 8-12 layers, cut into strips with a length of 1 cm and a width of 0.3-0.5 cm.

[0030] (2) Cage: loosen the cut maca leaves so that they are not pasted together, put them into a steamer covered with clean gauze in advance, the thickness is between 5-7 cm, so that it can be steamed evenly .

[0031] (3) Steam green: put water in the pot, after boiling, put the steamer in the pot and cover it tightly, steam quickly with high or rapid fire, after the atmosphere is turned on, switch to low heat for 2.5-5 minutes according to the degree of airtightness of the steamer , ope...

Embodiment 2

[0040] Embodiment two: the mensuration of maca leaf total flavonoids

[0041] Drawing of standard curve: Accurately weigh 10 mg of dried rutin standard sample and place it in a 50 mL volumetric flask, then dilute to volume with 80% ethanol and shake well to obtain a standard solution with a concentration of 0.2 mg / mL for use. Accurately pipette 0.0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 mL of rutin standard solution into 7 colorimetric tubes, add 5% NaNO respectively 2 0.3 mL of the solution, shake well, let it stand for 6 min, then add 10% Al(NO 3 ) 3 Solution 0.3mL, shake well, after standing for 6 minutes, add 4.0mL of 4% NaOH solution, then dilute to 10mL with 80% ethanol, shake well, let stand for 15 minutes, take the solution in the first test tube as blank reference , Measure the absorbance at 510 nm with a UV spectrophotometer. With the absorbance as the ordinate (Y) and the concentration (μg / mL) as the abscissa (X), the equation of the standard curve is obtained:

[00...

Embodiment 3

[0047] Example 3: Experimental Research on the Toxicology and Safety of Maca Leaf Black Tea

[0048] Objective: To test the safety of maca black tea as a health food.

[0049] Methods: Acute oral toxicity in mice, two mutagenic tests (mouse bone marrow micronucleus test, mouse sperm abnormality test) and 90-day feeding test.

[0050] result:

[0051] (1) Acute oral toxicity test of maca leaf black tea fine powder on SPF Kunming mice of two sexes, the total amount of two gavages in 12 hours was 10 g / kg·BW (equivalent to the recommended daily intake of the population) 300 times the dose of 0.033 g / kg·BW), no obvious symptoms of poisoning were observed in the animals within two weeks, and no animal died. According to the acute toxicity classification standard, the test substance belongs to the practical non-toxic level.

[0052] (2) The results of the two mutagenic tests (micronuclei in bone marrow cells and mouse sperm aberration) were all negative.

[0053] (3) Add maca l...

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Abstract

The invention discloses maca leaf black tea and a preparation method thereof. The maca leaf black tea is prepared by the following steps: removing stalks of selected maca leaves; loosening the cut maca leaves to prevent the maca leaves from sticking together; putting the maca leaves in a steamer paved with clean gauze in advance, wherein the stacking thickness of the maca leaves is 5-7cm and is proper for the uniform steaming of the maca leaves, wherein through the steaming process, the maca leaves are soft but bright green; immediately pouring the maca leaves into a clean container after taking the maca leaves out from the steamer, spreading and cooling, rolling and fermenting, wherein air temperature in a fermentation chamber is 24-25 DEG C, relative humidity is 95% and a leaf spreading thickness is 8-12cm; immediately drying the fermented maca leaves to prevent the tea leaves from being fermented continuously; spreading, sieving and packaging the tea leaves to obtain the maca leaf tea rich in total flavonoids. The tea disclosed by the invention can effectively eliminate a DPPH free radical,.OH and O2<->.; and the black team is obvious in dose-effect relationship and wide in application value.

Description

technical field [0001] The invention relates to the technical field of black tea preparation. Specifically, the present invention relates to the technical field of preparation of maca leaf black tea. Background technique [0002] Maca (Lepidium meyenii Walp) is an annual or biennial herb of the genus Brassicaceae, native to the Peruvian Andes at an altitude of 3500-4500. Maca is a medicinal and edible plant. It has been one of the main foods of the Incas living in the Andes for more than 2,000 years. It has the reputation of "Peruvian ginseng" and "South American ginseng". [0003] Modern studies have shown that maca is rich in nutritional and medicinal ingredients, mainly including macaene, macamide, maca alkaloids, glucosinolates, isothiocyanates and sterols. Maca has the effects of anti-oxidation, anti-fatigue, improving sexual function, promoting growth, and relieving menopausal syndrome. [0004] Flavonoids are a class of active substances widely found in plants. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/34
Inventor 闫论朱光辉牛新民
Owner 乳源瑶族自治县瑶山王茶业有限公司
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