Preparation method of piceatannol

A piceatannol and ethanol technology, applied in the biological field, can solve the problems of low purity of piceatannol and the like, and achieve the effects of easy industrial production, high product yield and simple operation.

Inactive Publication Date: 2014-12-10
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] By searching the literature, the literature on the preparation of picetanol from plants is less disclosed at present, such as the method disclosed in the patent "composition containing picetanol and the preparation method of the composition containing picetanol" is to extract and dry the organic reagent. Yes, the purity of picatanol is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 10 kg of passionflower seeds and grind them, add 2 times the amount of 60 ° C aqueous solution, add 0.1% cellulase for 3 hours, and then 8 times the amount of 60% ethanol solution for reflux extraction for 3 times, dilute the extract with water and add D101 large Adsorbed in a resin column with holes, firstly use 6 times column volume 40% ethanol solution to elute impurities, then use 10 times column volume 70% ethanol to elute active ingredients, eluate is concentrated without alcohol and placed for precipitation, and the precipitate is collected with 95% ethanol Dissolved and added to a short column of alumina, collected through the column, added twice the volume of water, left to crystallize, filtered out the crystals, and then refluxed with 95% ethanol to dissolve and recrystallize, and dried to obtain 9.5g of piceatanol, the content of which was 94.3% after testing .

Embodiment 2

[0019] Take 10kg of passionflower seeds and crush them, add 2 times the amount of 60°C aqueous solution, add 0.2% amylase for 3 hours, and then 8 times the amount of 60% ethanol solution for reflux extraction for 3 times, dilute the concentrated extract with water and add it to HZ816 macropore Adsorbed in the resin column, first use 6 times the column volume of 40% ethanol solution to elute the impurities, then use 10 times the column volume of 70% ethanol to elute the active ingredient, the eluent is concentrated without alcohol and placed for precipitation, and the collected precipitate is dissolved in 95% ethanol Add a short column of alumina, collect the column liquid and add twice the volume of water, let it crystallize, filter out the crystals, reflow with 95% ethanol to dissolve and recrystallize, and dry to obtain 10.2 g of piceatanol, with a detected content of 95.4%.

Embodiment 3

[0021] Take 10 kg of passionflower seeds and grind them, add 2 times the amount of 50 ° C aqueous solution, add 0.3% pectinase for 2 hours, and then reflux extract 5 times the amount of 90% ethanol solution for 3 times, dilute the extract with water and add AB- Adsorption in 8 macroporous resin columns, first use 6 times column volume 50% ethanol solution to elute impurities, then use 7 times column volume 80% ethanol to elute active ingredients, the eluent is concentrated without alcohol and placed for precipitation, and the precipitate is collected with 95 % ethanol was dissolved and added to a short column of alumina, the column liquid was collected and twice the volume of water was added, and the crystal was left to crystallize, the crystal was filtered out, and then refluxed with 95% ethanol to dissolve and recrystallize, and dried to obtain 8.9 g of piceatanol. 93.1%.

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PUM

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Abstract

The invention discloses a preparation method of piceatannol. The preparation method comprises the following steps: (1) crushing passion fruit seeds, adding 50-60 DEG C aqueous solution the amount of which is twice that of the passion fruit seeds, adding 0.1-0.5% of biological enzyme for enzymolysis, adding 50-90% ethanol solution for reflux extraction for 2-3 times, recovering the reagent from the extract and then adding the extract to a macroporous resin for adsorption, performing gradient elution by use of the ethanol solution, concentrating, standing and precipitating the eluate, and filtering to obtain the precipitate; (2) dissolving the precipitate by use of 95% ethanol, putting the solution through an aluminum oxide short column, collecting the column passing solution, adding 1-2 times of water by volume, stirring thoroughly and standing for crystallization, filtering out the crystals, performing reflux dissolution on the crystals by use of 95% ethanol for recrystallization, and drying the crystals to obtain the piceatannol. As the method is used for producing the piceatannol, the obtained piceatannol is high in purity, and the process is simple and easy to operate, high in extraction efficiency and easy for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a preparation method of piceatanol. Background technique [0002] Picetanol, CAS registration number 10083-24-6, molecular formula C14H12O4, molecular weight 244.24, molecular structure: [0003] [0004] Picetanol has a strong antioxidant effect, inhibiting non-receptor activating enzymes, spleen tyrosine kinase and lymphocyte-specific protein tyrosine kinase. Passiflora belongs to the Passiflora family. The fruit is rich in 17 kinds of amino acids, multivitamins, carotenoids and various trace elements needed by the human body. The soluble solids are 15-16%, and the total acid content is 3.8-4%. Moderately sweet and sour. Regular consumption of egg fruit and its processed products can help the human body digest, reduce phlegm, treat kidney deficiency, refresh the mind, activate blood and strengthen the body, calm and relieve pain, relieve pressure and reduce fat, etc. Seed Pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/68
CPCC07C37/004
Inventor 苏刘花杨存
Owner NANJING ZELANG AGRI DEV
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