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Method and application of immobilized glucoamylase by using sodium alginate silicon dioxide hybridized gel

A technology of sodium alginate and silicon dioxide, applied to biochemical equipment and methods, fixed on/in organic carriers, specific-purpose bioreactors/fermenters, etc., can solve the problem of loss of catalytic activity and contamination, Changes in the advanced structure of enzyme proteins, increasing immobilization costs, etc., to achieve the effects of reducing immobilization costs, saccharification costs, and protein content

Inactive Publication Date: 2014-12-10
SHANDONG XIWANG SUGAR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest defect of this method is that it often causes changes in the high-level structure of the enzyme protein and destroys the active center of the enzyme, thereby greatly reducing the immobilization rate of the enzyme; and the cross-linking method also uses expensive cross-linking reagents to further increase the immobilization rate cost
Adsorption method is also used for immobilization of glucoamylase. Adsorption method has many disadvantages, such as the selection of enzyme amount is based on experience, and weak binding force between enzyme and carrier may easily lead to loss of catalytic activity and contamination of reaction products, etc.
Therefore, its application is limited

Method used

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  • Method and application of immobilized glucoamylase by using sodium alginate silicon dioxide hybridized gel

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Experimental program
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Effect test

Embodiment 1

[0040] 1. Preparation of sodium alginate silica hybrid gel immobilized glucoamylase:

[0041] (1) Prepare 25ml of sodium alginate solution with a mass concentration of 2.0% (w / w) as liquid A for use after disinfection, and prepare 25ml of sodium silicate solution with a concentration of 1.5% (w / w) with sterile water and use a concentration of 30% The above acetic acid is adjusted to pH5.6-7.0 as B liquid for subsequent use;

[0042] (2) Mix liquid A and liquid B evenly to form liquid C, add 0.12 g of glucoamylase into solution C and stir for 10-30 minutes to obtain liquid D;

[0043] (3) The D solution after mixing is dripped into the calcium chloride solution with a mass concentration of 3% by a peristaltic pump through a 10ml syringe needle to form glucoamylase immobilized microsphere particles, soaked in the calcium chloride solution for 3 hours; then use distilled water Wash the microspheres and put them in a 4°C refrigerator for later use.

[0044] 2. The starch liquefa...

Embodiment 2

[0062] 1. Preparation of sodium alginate silica hybrid gel immobilized glucoamylase

[0063] (1) Prepare 4 liters of sodium alginate solution with a mass concentration of 1.8% (w / w) as liquid A for use after disinfection, and use sterile water to prepare 3.9 liters of sodium silicate solution with a mass concentration of 1.6% (w / w) Adjust the pH to 5.6-7.0 with hydrochloric acid and use it as liquid B for later use;

[0064] (2) Mix liquid A and liquid B evenly to form liquid C, add 15.3 g of glucoamylase into the scale of solution C and stir for 10-30 minutes to obtain liquid D.

[0065] (3) The D solution after mixing is dripped into the calcium chloride solution with a mass concentration of 4% by a peristaltic pump through a disc equipped with multiple syringe needles to form glucoamylase immobilized microsphere particles, soaked in the calcium chloride solution 3 hours; then wash the microspheres with distilled water and put them in a refrigerator at 4°C for later use.

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Abstract

The invention belongs to the technical field of immobilized enzymes, and in particular relates to a method of immobilized glucoamylase by using sodium alginate silicon dioxide hybridized gel, and a method for saccharifying the obtained immobilized glucoamylase to produce glucose. According to the method, the sodium alginate silicon dioxide hybridized gel is adopted to embed a saccharified enzyme so as to immobilize the enzyme, by virtue of most appropriate proportioning of silicon dioxide and sodium alginate, the defects that in a conventional embedding method, a substrate is not easy to contact with the enzyme, or enzyme molecules are easy to drop off, and the like, are overcome, the immobilization cost of the saccharified enzyme is lowered, the stability of the saccharified enzyme which is immobilized by using the embedding method is improved, and the production pollution is reduced. In addition, due to design of immobilized saccharified enzyme hydrolysis starch reaction equipment, functions of stirring a reaction liquid and separating the saccharified enzyme from the reaction liquid are achieved, and thus the immobilized saccharified enzyme can be repeatedly used.

Description

technical field [0001] The invention belongs to the technical field of immobilized enzymes, in particular to a method for immobilizing glucoamylase with sodium alginate silica hybrid gel, a method for saccharifying glucose with the obtained immobilized glucoamylase, and an immobilized enzyme reactor count. Background technique [0002] As a specific and efficient biocatalyst, enzymes have very broad and good application prospects. Because enzymes are expensive and free enzymes are difficult to recycle, effective immobilization of enzymes has always been a research hotspot in the field of enzyme engineering. [0003] In the process of cornstarch hydrolysis to produce glucose, a large amount of glucoamylase is used in the saccharification process, and the free glucoamylase cannot be recovered effectively after one use, which not only causes a lot of waste, but also increases the difficulty of the post-processing process and environmental pollution. [0004] The current status...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C12P19/14C12P19/02C12M1/02
CPCY02P20/50
Inventor 王棣李伟王居亮张继宽夏颖颖崔强高民光朱海涛
Owner SHANDONG XIWANG SUGAR
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