Hericium caput-medusae fermentation extract feed additive
A technology of feed additive and monkey head, which is applied in animal feed, animal feed, application, etc., and can solve problems that have not been seen yet
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Embodiment 1
[0012] The preparation of embodiment 1 Hericium erinaceus fermented extract
[0013] (1) Slant culture:
[0014] Quickly inoculate the pure strain of Hericium erinaceus into the special slant medium in the ultra-clean bench of the sterile room, and cultivate it at 26-28°C for 7-9 days to obtain the strain of the liquid shake flask; the formula of the special slant medium is: Potato 100: Glucose 1: Potassium dihydrogen phosphate 1: Agar 1, pH natural;
[0015] Preparation method: Peel 200 g of fresh potatoes and cut into small pieces, add 1000 ml of water and boil for 25 minutes, filter with gauze to get the filtrate, soak the agar in advance, add to the filtrate and boil while stirring continuously until it is completely dissolved, then add glucose and phosphoric acid respectively Potassium dihydrogen and other substances are stirred evenly, dispensed into test tubes while they are hot, corked and placed in a sterilizer for sterilization, steam pressure is 1.1 atmospheres, ...
Embodiment 3
[0039] Example 3 Fermented extract of Hericium erinaceus to promote growth of broiler chickens
[0040] 1. Materials and methods
[0041] 1.1 Experimental animals and grouping
[0042] Select 3,000 1-day-old Arbor Acres (AA) broilers, with a male-female ratio of half and half, and body weight (36.25±0.38). After observing no abnormality for 6 hours, they were randomly divided into 2 groups, with 3 repetitions in each group, and 500 rats in each repetition.
[0043] 1.2 Diet composition and nutrition of experimental animals
[0044] The test feed materials corn, soybean meal and calcium hydrogen phosphate meet the requirements of the national health standard (GB / 13078-2001). The diet was designed with reference to the national broiler feeding standard (NY / T33-1986) and NRC (1994) poultry feeding standard, and the fermented extract of Hericium erinaceus was added to the basic diet (daily No drug additives are added to the grain) and stir well.
[0045] 1.3 Date and manage...
Embodiment 4
[0051] Example 4 Effect of Hericium erinaceus fermented extract on immune function of broiler chickens
[0052] 1. Materials
[0053] The broilers used in the experiment were AA commercial broilers aged 14, 21 and 28 days. The broiler chicken group fed with the fermented extract of Hericium erinaceus was taken as the test group, and the addition amount was 0.3%; the broiler group fed without the fermented extract of Hericium erinaceus in the diet was the blank control group. 10 male and 10 hens were randomly selected from each group, slaughtered and peeled by bloodletting, their thymus, spleen and bursa of Fabricius were weighed respectively, and the immune organ index was calculated.
[0054] Immune organ index = immune organ mass / body weight × 100
[0055] 2. Data processing and analysis
[0056] The experimental data were statistically analyzed using SPSS 19.0 statistical software.
[0057] 3. Results and Analysis
[0058] The analysis results of thymus index, spleen...
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