Multimodal self-assembled nanoprobes for mri/pa and other imaging

A nano-probe and self-assembly technology, applied in the field of biological probes, can solve the problems that PET imaging positron non-metallic nuclides cannot be labeled, and the metal ion loading cannot be achieved, so as to achieve good tumor cell targeting and in vivo Clear fast, solve the effect of safety

Active Publication Date: 2017-04-12
JIANGSU INST OF NUCLEAR MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The first technical problem to be solved by the present invention is that there are no natural material nanoprobes suitable for PET / MRI / PA three-mode fusion imaging in the prior art, melanin that cannot realize PA imaging and metal ions in MRI imaging. Stable coexistence in aqueous solution, the loading of melanin on metal ions cannot achieve sensitive imaging, and the commonly used PET imaging positron non-metallic nuclides cannot be labeled, and then provide a PET / MRI / PA three-mode fusion imaging High-sensitivity, high-specificity nanoprobes for comprehensive imaging

Method used

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  • Multimodal self-assembled nanoprobes for mri/pa and other imaging
  • Multimodal self-assembled nanoprobes for mri/pa and other imaging
  • Multimodal self-assembled nanoprobes for mri/pa and other imaging

Examples

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Effect test

Embodiment 1

[0049] Example 1 Self-assembled PET / MRI / PA three-mode nanoprobe

[0050] The self-assembled PET / MRI / PA three-mode nanoprobe of this embodiment is: apoferritin is used as a carrier, loaded with 64 Cu, Fe for MRI 3+ , and melanin for PA imaging.

[0051] The synthesis route of the self-assembled PET / MRI / PA three-mode nanoprobe of this embodiment is as follows figure 1 Shown, its concrete preparation operation is as follows:

[0052] First, apoferritin and melanin are treated as follows: take 90nmol of apoferritin, add 3.6ml of water to dilute to obtain apoferritin solution, and set aside; dissolve 20mg of melanin in 10ml to a concentration of 0.1M (that is, mol / L) of NaOH aqueous solution, after dissolving, add 0.1M hydrochloric acid aqueous solution, adjust the pH value to 7, and then dissolve in ultrasonic to obtain a clear melanin aqueous solution. The above melanin aqueous solution was further purified in an ultrafiltration centrifugal filter with a molecular weight cut...

Embodiment 2

[0062] Example 2 Self-assembled PET / MRI / PA three-mode nanoprobe

[0063] The self-assembled PET / MRI / PA three-mode nanoprobe of this embodiment is: using ferritin as a carrier, loaded with 68 Ga, Gd for MRI 3+ , and melanin for PA imaging.

[0064] The preparation operation of the self-assembled PET / MRI / PA three-mode nanoprobe of this embodiment is as follows:

[0065] First, ferritin and melanin were pretreated according to the method and dosage in Example 1, and set aside.

[0066] (1) Take the above-mentioned ferritin solution, adjust the pH value to 1 with HCl, and obtain a dissociated protein solution;

[0067] (2) Add 90nmol of the above-mentioned standby melanin and 90 μmol of GdCl to the dissociated protein solution obtained in step 1), mix well, let stand for 15min, and then adjust the pH value to 10.0 with NaOH to obtain a recombinant protein solution; the resulting protein solution was passed through the PD-10 column, with PBS buffer (NaCl 137mmol / L, KCl 2.7mmol / ...

Embodiment 3

[0070] Example 3 Self-assembled PET / MRI / PA three-mode nanoprobe

[0071] The self-assembled PET / MRI / PA three-mode nanoprobe of this embodiment is: apoferritin is used as a carrier, loaded with 82 Rb, Fe for MRI 2+ , and melanin for PA imaging.

[0072] The preparation operation of the self-assembled PET / MRI / PA three-mode nanoprobe of this embodiment is as follows:

[0073] First, pretreat apoferritin and melanin according to the method and dosage in Example 1, and set aside. Among them, the particle diameter of melanin is 4 nm.

[0074] (1) Take the above-mentioned ferritin solution, adjust the pH value to 3 with HCl, and obtain the dissociated protein solution;

[0075] (2) Add 90nmol of the above-mentioned standby melanin and 135 μmol of FeCl to the dissociated protein solution obtained in step 1), mix well, leave for 15min, then adjust the pH value to 7.5 with NaOH to obtain a recombinant protein solution;

[0076] (3) adding radioactivity to the recombined protein so...

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Abstract

The invention discloses a multi-mode self-assembly nanoprobe. The multi-mode self-assembly nanoprobe takes apoferritin or ferritin as a carrier, and loaded with metal ions used for magnetic resonance imaging, melanin used for PA imaging, and function substances used for other imaging. The self-assembly nanoprobe disclosed by the invention realizes PET (polyethylene glycol terephthalate) / MRI / PA three-mode fusion development, has high sensitivity and specificity, realizes a good imaging effect, and can be widely applied clinically due to the simple process, good repeatability, good biocompatibility, safety and non-toxicity, fast elimination speed in a body.

Description

technical field [0001] The invention belongs to the field of biological probes, in particular to a multi-mode self-assembled nanometer probe suitable for MRI / PA and other imaging and its application. Background technique [0002] Molecular Imaging is one of the most promising medical frontier fields in the future. It includes a variety of medical imaging technologies, such as: positron emission tomography (PET), X-ray computer tomography (CT), nuclear magnetic Resonance imaging (MRI), optical imaging (including bioluminescence, fluorescence, near-infrared optical imaging), ultrasound imaging (US), etc. Different imaging technologies have their own characteristics and defects, so multi-mode fusion imaging has become a research and development hotspot today. With the rapid development of multimodal fusion imaging equipment, new technologies such as PET / CT, PET / MR, and photoacoustic imaging (PA, PAUS) have emerged one after another. The organic combination of functional infor...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K49/14A61K49/18A61K49/00A61K51/08A61K51/12A61K9/14
Inventor杨敏程震范曲立张瑞平
OwnerJIANGSU INST OF NUCLEAR MEDICINE