Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of sea anemone polysaccharide and its antitumor application

The technology of sea anemone polysaccharide and sea anemone is applied in the field of preparation of sea anemone anti-tumor active polysaccharide, which can solve the problems such as no report on the biological activity research of star worm Ai's sea anemone, and achieve obvious anti-tumor activity, good solubility, The effect of high cytotoxic selectivity

Active Publication Date: 2016-06-22
ZHEJIANG UNIV OF TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The coast of Zhejiang Province is rich in A. elegans sea anemone, but there is no report on the extraction method and biological activity of the polysaccharides of A.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of sea anemone polysaccharide and its antitumor application
  • A kind of preparation method of sea anemone polysaccharide and its antitumor application
  • A kind of preparation method of sea anemone polysaccharide and its antitumor application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] a) Raw material processing: clean the outer surface of the fresh star worm, cut it, wash off the contents of its coelenterate, and cut it into pieces;

[0041] b) cutting the homogenate of crushed sea anemone for 10 minutes, then adding water with a volume 4 times the volume of the homogenate to the homogenate, and leaching at 30° C. for 2 hours to obtain an extract;

[0042] c) centrifugation of the extract: 8000rpm, 4°C, centrifugation for 5min, to obtain a supernatant;

[0043] d) Concentration: Concentrate the supernatant by rotary evaporation under reduced pressure (45° C.) to 1 / 4 of the original volume to obtain a concentrated solution;

[0044]e) Alcohol precipitation: add 95% ethanol to the concentrated solution until the volume fraction of ethanol is 30%, place it at 4°C for 4 hours, centrifuge and separate to obtain the precipitate I and supernatant I, and continue to add to the supernatant I Add 95% ethanol until the ethanol volume fraction is 60%, let it st...

Embodiment 2

[0049] a) Raw material processing: clean the outer surface of the fresh star worm, cut it, wash off the contents of its coelenterate, and cut it into pieces;

[0050] b) Cut the homogenate of crushed sea anemone for 15 minutes, add 6 times the volume of water to the homogenate, and extract at 50° C. for 3 hours to obtain an extract;

[0051] c) centrifugation of the extract: 9000rpm, 4°C, centrifugation for 10min to obtain a supernatant;

[0052] d) Concentration: Concentrate the supernatant by rotary evaporation under reduced pressure (45° C.) to 1 / 4 of the original volume to obtain a concentrated solution;

[0053] e) Alcohol precipitation: add 95% ethanol to the concentrated solution until the volume fraction of ethanol is 30%, place it at 4°C for 6 hours, centrifuge and separate to obtain the precipitate I and supernatant I, and continue to add to the supernatant I Add 95% ethanol until the ethanol volume fraction is 60%, let it stand for the same time, centrifuge and sep...

Embodiment 3

[0058] a) Raw material processing: clean the outer surface of the fresh star worm, cut it, wash off the contents of its coelenterate, and cut it into pieces;

[0059] b) Cut the homogenate of crushed sea anemone for 20 minutes, add 10 times the volume of water to the homogenate, and extract at 70° C. for 4 hours to obtain an extract;

[0060] c) centrifugation of the extract: 10000rpm, 4°C, centrifugation for 15min to obtain a supernatant;

[0061] d) Concentration: Concentrate the supernatant by rotary evaporation under reduced pressure (45° C.) to 1 / 4 of the original volume to obtain a concentrated solution;

[0062] e) Alcohol precipitation: add 95% ethanol to the concentrated solution until the volume fraction of ethanol is 30%, place it at 4°C for 8 hours, centrifuge and separate to obtain the precipitate I and supernatant I, and continue to add to the supernatant I Add 95% ethanol until the ethanol volume fraction is 60%, let it stand for the same time, centrifuge and s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of sea anemone polysaccharides. The raw materials are broken by a constant temperature high-speed shearing method, subjected to water leaching, 30%, 60%, and 80% alcohol precipitation, and then enzymatically hydrolyzed with papain, and desorbed by sevag. Protein, three kinds of sea anemone polysaccharides with higher sugar content are obtained, and the three kinds of sea anemone polysaccharides prepared by the present invention can be used to prepare antitumor drugs, specifically can be used to prepare anti-lung cancer, anti-prostate cancer or ascites tumor drugs.

Description

1. Technical field [0001] The invention relates to the field of deep processing of marine products, in particular to a preparation method of sea anemone polysaccharide with anti-tumor activity. 2. Background technology [0002] The environment of high salinity, high pressure, low temperature, hypoxia, oligonutrition and insufficient light in the ocean has contributed to the richness and diversity of the structure and function of marine polysaccharides. Numerous studies have shown that marine animal polysaccharides have anti-tumor, anti-virus, anti-cardiac Vascular diseases, anti-oxidation and immune regulation and other biological activities, such as: abalone polysaccharides (Yin Hongling, Yang Jingfeng. Research on Enzymatic Extraction of Abalone Polysaccharides, Food and Fermentation Industry, 2006, 32(12): 158), sea cucumber polysaccharides (Cai Binxin , Wu Chengye. Separation and purification of polysaccharides from sea cucumbers and their main biological activities, Fuj...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 何荣军赵月钧王茜孙培龙
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com