Enterobacter cancerogenus for degrading polyethylene and application of enterobacter cancerogenus

A technology of Enterobacteriaceae, which is Enterobacteriaceae, is applied in the fields of biotechnology and the environment, which can solve the problems of unstable degradation effect, large energy consumption, and high cost, achieve significant economic and environmental protection benefits, and increase landfill capacity.

Active Publication Date: 2014-12-24
BEIHANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is costly, consumes a lot of energy, and the degradation effect is unstable.

Method used

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  • Enterobacter cancerogenus for degrading polyethylene and application of enterobacter cancerogenus
  • Enterobacter cancerogenus for degrading polyethylene and application of enterobacter cancerogenus
  • Enterobacter cancerogenus for degrading polyethylene and application of enterobacter cancerogenus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Screening and isolation of Enterobacter cancerogenus YZ1

[0036]Disinfect the bodies of plastic-gnawing insects, use 75% (v / v) ethanol to clean the bodies, and then rinse them twice with sterile water to wash away the alcohol on the insects until the bodies are clean. Work in Petri dishes under sterile conditions. Take out the sterilized worm body, and pull out its intestine after dissection. Afterwards, drop a drop of normal saline on the side of the intestinal tract, gently drive out the intestinal fluid with the head of the elbow tweezers, extract the intestinal fluid with a pipette, and then dilute it 5 times with 0.85% normal saline to make a suspension of intestinal bacteria. Add 1ml of the prepared intestinal bacteria suspension into the Erlenmeyer flask, and then measure 19ml of sterilized liquid inorganic salt medium into the Erlenmeyer flask to prepare 20ml liquid culture intestinal bacteria liquid. The formula of liquid inorganic salt medium is: KH 2 P...

Embodiment 2

[0040] Enterobacter YZ1 degrades plastic in solid inorganic salt medium:

[0041] 1. Take the Enterobacter YZ1 screened in Example 1 and place it in a 150ml Erlenmeyer flask filled with 20ml of broth medium, and culture it at 28°C for 1 to 2 days at a shaker speed of 150rpm. Then inoculate into a 250ml Erlenmeyer flask equipped with 50ml of broth medium, and ferment and cultivate under the same conditions for 1 day to obtain bacterial liquid. Centrifuge and resuspend in normal saline three times, wash away the original medium, and ensure that there is no carbon source and other nutrients in the bacterial suspension. Determination of OD of bacterial suspension 600 value and diluted to a reasonable multiple to obtain a concentration of 1 to 3×10 6 CFU / ml bacterial suspension.

[0042] 2. Use a pipette to take 5mL of the bacterial suspension of the above concentration, and drop it in the center of the solid inorganic salt medium for coating culture. After the coating is compl...

Embodiment 3

[0046] Enterobacter YZ1 degrades plastic in liquid inorganic salt medium:

[0047] Enterobacter YZ1 screened in Example 1 was prepared according to the method in Example 2 to obtain a bacterial suspension. Add 10ml of the bacterial suspension and 40ml of liquid inorganic salt medium to a 250ml Erlenmeyer flask, cut the polyethylene film into small pieces (about 0.5mm×0.5mm) with scissors, add it to the Erlenmeyer flask, and shake at 28°C The bed was rotated at 100 rpm for culture. On the 7th day, 14th day, 21st day, 28th day, and 60th day, 3 parallels were set up. At the corresponding time point, the polyethylene was taken out, weighed and the OD of the bacterial solution was determined 600 value. OD at each time point 600 Value results like Figure 6 As shown, the bacterial cell concentration in the 60-day culture showed an increasing trend, which was similar to the CFU count. The weighed weight of polyethylene plastic at each time point is as follows: Figure 7 shown ...

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Abstract

The invention discloses an enterobacter cancerogenus for degrading polyethylene and an application of the enterobacter cancerogenus. The bacterial strain disclosed by the invention is enterobacter cancerogenus YZ1 CGMCC No.6816. The active component of a microbial agent disclosed by the invention is enterobacter cancerogenus YZ1 CGMCC No.6816. The bacterial strain disclosed by the invention is capable of effectively corroding and degrading polyethylene plastic. The microbial agent disclosed by the invention is low in production cost, and can degrade 5% (mass percent) of polyethylene within 60 days. The bacterial strain and the microbial gent disclosed by the invention can be suitable for polyethylene wastes in refuse landfills and various water body and soil environments, and can ensure that the polyethylene can be biodegraded. According to the method disclosed by the invention for efficiently degrading microorganisms of plastic, the microorganisms of degradable plastic with high purity and high activity can be quickly obtained. A technique and a method can be provided for solving the problems that the existing polyethylene plastic wastes pollute the environment and occupy great landfill capacity of the landfill, and the enterobacter cancerogenus is of great importance in environmental protection and reduction of garbage disposal cost.

Description

technical field [0001] The invention relates to the fields of biotechnology and environmental technology. Specifically, the present invention relates to a method for screening microorganisms capable of rapidly degrading plastics and uses of the microorganisms. Background technique [0002] With the continuous development of human society, the increase of population and the continuous advancement of technology, plastics have been applied in all aspects of human life and production. Due to its excellent performance, polyethylene has become a widely used and extremely important product in people's life and production. It is the highest-yield variety among synthetic resins, accounting for about a quarter of the total plastic output. Global polyethylene production in 2011 has reached 1.32×10 9 Ton. However, because polyethylene is extremely difficult to degrade in the natural environment, how to deal with waste polyethylene has become a big problem. [0003] Currently, incine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B1/00C12R1/01
Inventor 杨军魏轶丰赵姣杨宇周丽沙杨仁涛汪祥燕苏晴晴于忆潇周宇平杨瑞馥
Owner BEIHANG UNIV
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