Method for preparing fluorescent silica nanoparticles by ultraviolet light radiation

Abstract

The invention discloses a method for preparing fluorescent silica nanoparticles by ultraviolet light radiation, which comprises the following steps of adding a silicon source compound into an aqueous solution of a reducing compound; adding a ligand compound, so that a precursor solution is obtained; and radiating the obtained product under ultraviolet light, so that water soluble fluorescent silica nanoparticles are obtained. The method disclosed by the invention is implemented completely in an aqueous phase, and therefore, the method is safe in operation, fast and convenient, low in toxicity, and safe and easily obtained in raw materials. The obtained fluorescent silica nanoparticles are good in monodispersity, high in fluorescence properties and quantum yield and good in stability, has good water solubility, can be used as fluorescent markers and widely applied to biological detection and analysis, and is expected to be used in related optical industrial productions.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biological analysis and detection, and relates to a method for preparing silicon nanoparticle, in particular to a method for preparing fluorescent silicon nanoparticle by ultraviolet (UV) radiation. Background technique [0002] Due to their unique optical / electrical / mechanical properties, surface modification and good biocompatibility, functional silicon nanomaterials have increasingly attracted widespread attention from scientists (see: Nature 2000, 408, 440-444; Science 2002, 296, 1293-1297; Science 2003, 299, 1874-1877; Science 2006, 313, 1100-1104.). Of particular concern is that fluorescent silicon nanoparticles, as a representative zero-dimensional silicon nanomaterial, are expected to be widely used in biology and medicine because of their good biocompatibility and low toxicity (see: Science 2005, 307, 538-544; Chem. Roc. Rev. 2010, 39, 4234-4243; Nano Today 2010, 5, 282-295.). ...

AI Technical Summary

Problems solved by technology

However, although these methods can greatly enhance the water dispersibility of silicon nanoparticles, the process is relatively complicated (for example, it is first necessary to prepare hydrophobic silicon nanoparticles and then modify them with hydrophilic groups). , and the modification process is easy to damage the surface of silicon nanoparticles, affecting its physical / chemical properties (acrylic acid / allylamine modified silicon nanoparticles have poor pH stability; polymer / microbeam modified silicon nanoparticles are too large in size (60~ 300nm)) (See: Angew. Chem. Int. Ed. 2009, 121, 134-138; Nano Today 2010, 5, 282-295; ACS Nano 2011, 5, 413-423)

In recent years, although there are also reports on water-soluble silicon nanoparticles, most of them are from large to small (that is, top-down, using large silicon nanoparticles, silicon oxide powder, and silicon nanowires as silicon sources). The preparation method, the size of the prepared silicon nanoparticles is difficult to control, and the fluorescence quantum efficiency is relatively low (see: J. Am. Chem. Soc. 2011, 133, 14192-14195; Angew. Chem. Int. Ed. 2012, 51 , 8485-8489.)

This largely limits the development of silicon nanoparticle-based biological applications

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