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Pre-treatment method for microbial oil physical refining

A microbial oil and physical refining technology, applied in the direction of oil/fat refining, fat production, and fat oil/fat production, etc., can solve the problems of high moisture content in dry cells, can not guarantee the passivation of phospholipase, etc., and achieve the effect of improving the effect.

Inactive Publication Date: 2015-01-07
青岛海智源生命科技有限公司
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Problems solved by technology

[0005] The Chinese invention patent with the application number 200910085128 discloses "a preparation method of microbial oil", which is characterized by extruding and puffing dry bacteria, and obtaining mixed oil and extracting bacteria meal by leaching the extruded and expanded dry bacteria with No. 6 solvent; However, the moisture content of the dried bacteria is high, and neither the heating steam pressure nor the extruding temperature can guarantee the inactivation of phospholipase. The subsequent process uses No. 6 solvent for leaching treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Collect 100 tons of microbial cell culture fluid after fermentation, press filter the obtained microbial cell culture fluid to obtain 60 tons of filter cake;

[0024] (2) 60 tons of filter cakes obtained are flash-dried in advance to remove most of the free water and part of bound water to obtain 20 tons of dry filter cakes;

[0025] (3) Crush 20 tons of dry filter cake and send it to an extruder with a pre-pressing section. The pressure of the hydraulic disc is 16MPa, and hot steam with a pressure of 0.9MPa is added. The puffing temperature reaches 155°C and the residence time is 9s. Stage oil extraction, the obtained microbial oil is hydrated and degummed, and the measured phosphorus content is 10ppm.

Embodiment 2

[0027] (1) Collect 100 tons of microbial cell culture fluid after fermentation, press filter the obtained microbial cell culture fluid to obtain 60 tons of filter cake;

[0028] (2) 60 tons of filter cakes obtained are flash-dried in advance to remove most of the free water and part of bound water to obtain 20 tons of dry filter cakes;

[0029] (3) Crush 20 tons of dry filter cake and send it to an extruder with a pre-pressing section. The pressure of the hydraulic disc is 18MPa, and hot steam with a pressure of 1.5MPa is added. The puffing temperature reaches 165°C and the residence time is 8s. Stage oil extraction, the microbial oil obtained after hydration and degumming, the measured phosphorus content is 8ppm.

Embodiment 3

[0031] (1) Collect 100 tons of microbial cell culture fluid after fermentation, press filter the obtained microbial cell culture fluid to obtain 60 tons of filter cake;

[0032] (2) 60 tons of filter cakes obtained are dried in advance to remove most of the free water and part of bound water to obtain 20 tons of dry filter cakes;

[0033] (3) Crush 20 tons of dry filter cake and send it to an extruder with an oil extraction section. The pressure of the hydraulic disc is 19MPa, and hot steam with a pressure of 1.7MPa is added. The expansion temperature reaches 180°C and the residence time is 6s. Stage oil extraction, the microbial oil obtained after hydration and degumming, the measured phosphorus content is 9ppm.

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Abstract

The invention relates to a pre-treatment method for microbial oil physical refining. The pre-treatment method comprises the following steps of 1, collecting a microbial bacterium culture solution and filtering the microbial bacterium culture solution by a membrane filter press to obtain filter cake, 2, carrying out advance flash evaporation drying on the filter cake to remove most of free water and a part of bound water, and 3, under the conditions of high heat-humidity, carrying out puffing treatment on the filter cake obtained by the step 2 by a bulking machine with a pre-pressing section. The oil extracted from the collected microbial bacteria is subjected to high heat-humidity puffing treatment so that oil quality and safety are guaranteed and enzymes in the oil are effectively passivated. The crude oil has reduced nonhydratable phospholipid content and improved total phospholipid content so that follow-up physical refining hydration degumming effects are substantially improved. The hydrated degummed oil has phospholipid content reduced to 0.01-0.03wt% which is equivalent to phosphor content less than 10ppm so that oil refining requirements are satisfied.

Description

technical field [0001] The invention relates to the technical field of microbial oil treatment, in particular to a pretreatment method suitable for physical refining of microbial oil. Background technique [0002] Microbial oil is a new type of oil that has been widely studied and widely used. Some of it has entered people's lives as edible oil, especially for infants, pregnant women, intellectual workers and other specific consumer groups. It plays an important role in improving people's nutrition and health. effect. At present, the most researched and most widely used are docosahexaenoic acid (DHA), arachidonic acid (ARA), eicosapentaenoic acid (EPA), etc., which are effective for the nervous system and vision of infants and young children. It plays an important role in the healthy development of the system, enhancing memory and thinking ability, improving intelligence and promoting brain development. [0003] Expansion is a processing technology that depressurizes the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/06C11B1/02
CPCC11B1/025C11B3/001
Inventor 白长军朱蓓薇金青哲王兴国王永富初跃峰戚桂斌白红超
Owner 青岛海智源生命科技有限公司
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