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A high-throughput method for rapid screening of immune enhancers of Macrobrachium rosenbergii

An immune enhancer, the technology of Macrobrachium rosenbergii, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high risk, strong specificity, long cycle of immune enhancer, etc., to reduce the breeding conditions , the operation steps are simple, the effect of saving the cost of breeding

Active Publication Date: 2016-03-23
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the process of disease prevention and control of shrimp and crabs, antibiotics and some chemical drugs are mainly used in large quantities. Although they have a certain effect on related diseases and alleviate the occurrence of some diseases to a certain extent, the frequent and large-scale use of antibiotics leads to drug resistance. , large toxic and side effects, decreased immunity of shrimp and crabs, and drug residues have caused many problems to human health
As a result, vaccines and immune enhancers have become the first choice for the prevention and treatment of aquatic animal diseases. Among them, vaccines have a significant preventive effect on various diseases, especially viral diseases, but they have strong specificity and poor operational feasibility in production. It is an immune enhancer with unique effects on the non-specific immunity of animals. It has become a research hotspot in aquaculture, and it is also in line with the new trend of healthy ecological farming in the aquaculture industry.
However, the long cycle, high cost, and high risk of screening immune enhancers through breeding, and the strong emergency response of shrimp and crabs, resulting in instability of various indicators, all of which affect the screening of immune enhancers

Method used

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  • A high-throughput method for rapid screening of immune enhancers of Macrobrachium rosenbergii
  • A high-throughput method for rapid screening of immune enhancers of Macrobrachium rosenbergii
  • A high-throughput method for rapid screening of immune enhancers of Macrobrachium rosenbergii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A high-throughput method for rapidly screening an immune enhancer for Macrobrachium rosenbergii, comprising the steps of:

[0042] 1. Treatment of immune enhancers to be screened

[0043] Immunopotentiators to be screened select some immunopotentiators that have the effects of improving immunity and antibacterial effects recorded in existing literature: Lycium barbarum polysaccharide, lentinan, tremella polysaccharide, laminarin, bamboo leaf flavonoids, baicalin, levamisole, astragalus polysaccharide, Cordyceps polysaccharides, Ganoderma lucidum polysaccharides, Tea tree polysaccharides, Flammulina velutipes polysaccharides, Yunzhi polysaccharides, Tremella polysaccharides, vitamin C, chitin, chitosan, pine pollen, yeast wall polysaccharides, (-dextran, etc. total 20 kinds.

[0044] Mix the immunopotentiator to be screened with sterilized PBS buffer (NaCl137mmol / L, KCl2.7mmol / L, NaCl 2 HPO 4 10mmol / L, KH 2PO 4 2mmol / L, pH7.4) and mixed to prepare a solution of immun...

Embodiment 2

[0058] A high-throughput method for rapidly screening an immune enhancer for Macrobrachium rosenbergii, comprising the steps of:

[0059] 1. Treatment of immune enhancers to be screened

[0060] The immune enhancer to be screened selects some immune enhancers recorded in the existing literature: Lycium barbarum polysaccharide, Lentinan polysaccharide, Tremella polysaccharide, laminarin, bamboo leaf flavonoids, baicalin, levamisole, astragalus polysaccharide, Cordyceps polysaccharide, Ganoderma lucidum polysaccharide, tea tree mushroom polysaccharide , Flammulina velutipes polysaccharide, versicolor polysaccharide, tremella polysaccharide, vitamin C, chitin, chitosan, pine pollen, yeast wall polysaccharide, (-dextran, etc. a total of 20 kinds.

[0061] Mix the immunopotentiator to be screened with sterilized PBS buffer (NaCl137mmol / L, KCl2.7mmol / L, NaCl 2 HPO 4 10mmol / L, KH 2 PO 4 2mmol / L, pH7.4) to prepare a solution of the immune enhancer to be screened with a concentrati...

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Abstract

The invention discloses a high-flux quick screening method of Macrobrachium rosenbergii immunopotentiators. The method comprises the following steps: treatment of immunopotentiators to be screened, acquisition and culture of Macrobrachium rosenbergii haemolymph cells, and detection and analysis of Macrobrachium rosenbergii nonspecific immunity indexes. The method can be used for in-vitro quick high-flux screening of the Macrobrachium rosenbergii immunopotentiators.

Description

technical field [0001] The invention relates to an in vitro screening method for an immune enhancer of Macrobrachium rosenbergii, in particular to a method for rapidly screening an immune enhancer for Macrobrachium rosenbergii with high throughput. Background technique [0002] Macrobrachium rosenbergii ( Macrobrachium rosenbergii ), also known as Malaysian prawns and freshwater long-arm prawns, belong to the phylum Arthropada, Crustacea, Decapoda, Palaemonidae, and Macrobrachium in classification , is a large freshwater economical shrimp. Native to tropical and subtropical regions of the Indian Ocean and Pacific Ocean. Macrobrachium rosenbergii was introduced to China from Japan in 1976. After more than 30 years of breeding, especially since the 1990s, with the improvement of artificial breeding technology and the development of breeding technology, the breeding area and production have increased rapidly. By 2009 , my country's annual growth rate of Macrobrachium rosenber...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/42C12Q1/28C12Q1/26
Inventor 林锋沈锦玉刘莉曹铮郝贵杰盛鹏程
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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