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Rapid detection method for pesticide residues in fruits and vegetables

A technology for pesticide residues and detection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome pretreatment, damage to samples, and long time consumption, and achieve the effects of less consumption of organic solvents, pollution prevention, and high recovery rate

Inactive Publication Date: 2015-01-14
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to: in view of the above existing problems, provide a rapid detection method for pesticide residues in fruits and vegetables, which solves the problems of sample damage, cumbersome pretreatment, long time-consuming, high cost, and environmental pollution caused by conventional pesticide residue detection methods. Insufficient problems, it has the advantages of simple operation, fast, low cost, less consumption of organic solvents, high enrichment efficiency, reliable sensitivity, good recovery rate, etc., and can meet the international detection requirements for pesticide residue limits in vegetables. certain promotional value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] After washing the apples, chop or mash them, weigh 10g in a centrifuge tube, first add 2g of sodium chloride, 10g of anhydrous sodium sulfate, and then add 15mL of acetone and dichloromethane mixed solvent in a ratio of 1:1, Shake vigorously for 1 min, after ultrasonic extraction for 10 min, centrifuge at 5000r / min for 1 min, take out the organic supernatant for later use, and then extract the residue twice with 10mL and 5mL organic solvents respectively, and combine the extracts; place the obtained extracts in a distillation flask, Evaporate to dryness under vacuum in a water bath at 40°C or blow dry with nitrogen; add 1mL of acetone to dissolve, then elute through a CARBON / NH2 column, using a mixed solvent of acetone and n-hexane with a volume ratio of 3:5, acetone, and ethyl acetate , 2 mL of acetone and methanol mixed solvents with a volume ratio of 1:1 for gradient elution, combined to obtain a purified solution, concentrated and evaporated to dryness, added a mixtu...

Embodiment 2

[0021] Wash and chop cabbage leaves, weigh 12g in a centrifuge tube, first add 3g of sodium chloride, 5g of anhydrous sodium sulfate, then add 15mL of acetone and dichloromethane mixed solvent in a ratio of 1:1, Shake vigorously for 1 min, after ultrasonic extraction for 10 min, centrifuge at 5000r / min for 1 min, take out the organic supernatant for later use, and then extract the residue twice with 10mL and 5mL organic solvents respectively, and combine the extracts; place the obtained extracts in a distillation flask, Evaporate to dryness under vacuum in a water bath at 40°C or blow dry with nitrogen; add 1mL of acetone to dissolve, then elute through a CARBON / NH2 column, using a mixed solvent of acetone and n-hexane with a volume ratio of 3:5, acetone, and ethyl acetate , 2 mL of acetone and methanol mixed solvent with a volume ratio of 1:1 for gradient elution, combined to obtain a purified solution, concentrated and evaporated to dryness, added a mixture of acetone and wat...

Embodiment 3

[0023] Peeled oranges, chopped or crushed, weighed 6g in a centrifuge tube, first added 3g of sodium chloride, 8g of anhydrous sodium sulfate, and then added 16mL of acetone and dichloromethane mixed solvent in a ratio of 1:1, vigorously Vibrate for 1 min, ultrasonically extract for 10 min, centrifuge at 5000 r / min for 1 min, take out the organic supernatant for later use, and then use 10 mL and 5 mL of organic solvents for ultrasonic extraction twice, and combine the extracts; ℃ in a water bath by vacuum rotary evaporation or blowing to dryness with nitrogen; add 1mL of acetone to dissolve, and then elute through a CARBON / NH2 column, using acetone and n-hexane mixed solvent with a volume ratio of 3:5, acetone, ethyl acetate, Gradient elution with 2 mL each of acetone and methanol mixed solvents with a volume ratio of 1:1, combined to obtain the purified solution, concentrated and evaporated to dryness, adding a mixture of acetone and water with a volume ratio of 1:1 to constan...

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Abstract

The invention discloses a rapid detection method for pesticide residues in fruits and vegetables, and relates to the field of food safety. The method comprises four steps: ultrasonic extraction, concentration and drying by distillation, purification and GC-MS / MS determination. According to the rapid detection method, the problems of damages to samples, fussy pretreatment, long elapsed time, high cost, pollution to environment and the like in a conventional pesticide residue detection method are solved, and the rapid detection method has the advantages of being simple and rapid in operation, low in cost, low in organic solvent consumption, high in enrichment efficiency, reliable in sensitivity, relatively high in recovery rate and the like, can meet the international detection requirements on the pesticide residue limits in vegetables, and has certain promotional value.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a rapid detection method for pesticide residues in fruits and vegetables. Background technique [0002] With the development of agricultural industrialization, the production of agricultural products is increasingly dependent on exogenous substances such as pesticides, antibiotics and hormones. The amount of pesticides used in food, vegetables, fruits, and tea remains high in China, and the unreasonable use of these substances will inevitably lead to excessive pesticide residues in agricultural products, affecting the safety of consumers, and in severe cases, it will cause consumers to treat diseases and develop diseases. Abnormal, even directly lead to poisoning death, excessive pesticide residues will also affect the trade of agricultural products. With the improvement of people's living standards, consumers put forward higher and higher requirements for the food safety of fruits an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张艳梅
Owner GUANGXI UNIV