Device and method for determining nanometer material through hollow fiber field-flow fractionation
A technology of flow field flow separation and nanomaterials, which is applied in the field of environmental analytical chemistry to achieve the effects of high separation efficiency, low operating cost and easy operation
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Embodiment 1
[0044] Embodiment 1: Polystyrene (polystyrene, be called for short PS) microsphere separation assay
[0045] Polystyrene (PS) microspheres are widely used in nanoscale standard particles because of their high monodispersity, ideal spherical shape, easy-to-control particle size and surface electrical properties. The company has commercialized PS microspheres for sale. The microspheres of the present invention are purchased from the National Institute of Standards and Technology (abbreviated as NIST) of two kinds of PS microspheres with particle diameters of about 50nm and 100nm. During the experiment, the maximum absorption wavelength of the ultraviolet detector was selected as 254nm. The hollow fiber flow field flow separation device is used to separate the mixture of two PS microspheres, and the resolution R is 1.8. It can be seen that the device of the present invention can be used for the separation of PS microspheres with different particle sizes.
Embodiment 2
[0046] Embodiment 2: Separation and determination of synthetic gold nanoparticles
[0047] In the early days, gold nanoparticles were used as drugs, such as to treat alcoholism, tuberculosis, arthritis and so on. Since the 20th century, with the maturity of the synthesis method of nano-gold particles and the in-depth research on the characteristics of nano-gold optics, electricity and biological affinity, the application fields of nano-gold have become more extensive. The hollow fiber flow field flow separation device was used to separate two kinds of self-prepared gold nanoparticles. During the experiment, the maximum absorption wavelength of the ultraviolet detector was selected as 520nm. With the device of the present invention, gold nanoparticles with two sizes of 5nm and 60nm are better separated (R=2.5).
Embodiment 3
[0048] Embodiment 3: Separation and determination of nano-silver in sewage treatment plant outlet wastewater
[0049] In addition to applications in the electronics industry and catalysis, nano-silver is widely used in medicine and personal care because of its good bactericidal properties. Nanosilver will inevitably be released into the environment during production and use. The device of the invention is used to separate and measure the nano-silver in the outlet wastewater of a sewage treatment plant. During the experiment, the maximum absorption wavelength of the ultraviolet detector was selected as 410nm. The recoveries of the standard sample were 85% (20nm), 92% (40nm) and 102% (60nm), and the resolution R of the three particle sizes of nano silver was greater than 1.7. The device of the invention will have wide application prospects in the fields of environmental chemistry, environmental detection research and the like.
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