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Method for preparing peanut non-starch polysaccharides by utilizing hot pressed peanut meal

A technology of non-starch polysaccharide and hot-pressed peanut meal, which is applied in the field of food nutrient active substance processing, can solve the problems of low efficiency, easily damaged structure, unsuitable for industrial production and the like

Inactive Publication Date: 2015-01-28
青岛博之源生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hot water extraction consumes a lot of energy, has low efficiency, and requires multiple extractions. Subsequent removal of water in the extraction solution consumes a lot of energy and takes a long time, which is not suitable for industrial production.
In the ethanol solution extraction method, the structure of peanut non-starch polysaccharides is easily destroyed in ethanol for a long time, the extraction rate is low, and the product activity is low
The acid and alkali solution extraction method is harsh, the peanut non-starch polysaccharide is easily denatured, and the production waste liquid is easy to pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Peanut meal is crushed through a 100-mesh sieve, and the sieve is taken as a raw material. Use peanut meal powder, add distilled water to the peanut meal powder at a ratio of 1:8.9 (g / ml), and use a microwave power of 900w in a microwave catalytic / synthesizer. , Heating temperature at 50.7°C, microwave heating for 1.5min to mix peanut meal powder and distilled water evenly, adjust the pH value of the mixture to 5.8, add 15.3 FBG per gram of peanut meal powder (FBG is the enzyme activity unit of the compound plant hydrolytic enzyme Viscozyme L) Viscozyme L was hydrolyzed for 5.2 minutes in a microwave catalytic / synthesizer with a microwave power of 900w and a heating temperature of 50.7°C to obtain a Viscozyme L enzymatic hydrolyzate; adjust the pH of the Viscozyme L enzymatically to 8.4, and add The compound protease of peanut meal powder 4756U (U is the enzyme activity unit of the compound protease mixed with alkaline protease Alcalase and trypsin at a ratio of 2.8:1.5)...

Embodiment 2

[0018] Peanut meal is crushed through a 100-mesh sieve, and the sieve is taken as a raw material. Use peanut meal powder, add distilled water to the peanut meal powder at a ratio of 1:9.2 (g / ml), and use a microwave power of 900w in a microwave catalytic / synthesizer. , Heating temperature at 50.7°C, microwave heating for 1.5min to mix peanut meal powder and distilled water evenly, adjust the pH value of the mixture to 5.7, add 16.4 FBG per gram of peanut meal powder (FBG is the enzyme activity unit of the compound plant hydrolytic enzyme Viscozyme L) Viscozyme L was hydrolyzed for 5.2 minutes in a microwave catalytic / synthesizer with a microwave power of 900w and a heating temperature of 50.7°C to obtain a Viscozyme L enzymatic hydrolyzate; adjust the pH of the Viscozyme L enzymatically to 8.6, and add The compound protease of peanut meal powder 5658U (U is the enzymatic activity unit of the compound protease mixed with alkaline protease Alcalase and trypsin at a ratio of 2.8:1...

Embodiment 3

[0020]Peanut meal is crushed through a 100-mesh sieve, and the sieve is taken as a raw material. Use peanut meal powder, add distilled water to the peanut meal powder at a ratio of 1:9.5 (g / ml), and use a microwave power of 900w in a microwave catalytic / synthesizer. , Heating temperature at 50.7°C, microwave heating for 1.5min to mix peanut meal powder and distilled water evenly, adjust the pH value of the mixture to 5.9, add 17.2 FBG per gram of peanut meal powder (FBG is the enzyme activity unit of the compound plant hydrolytic enzyme Viscozyme L) Viscozyme L was hydrolyzed for 6.4 minutes in a microwave catalytic / synthesizer with a microwave power of 900w and a heating temperature of 50.7°C to obtain a Viscozyme L enzymatic hydrolyzate; adjust the pH of the Viscozyme L enzymatically to 8.8, and add Peanut meal powder 6268U (U is the enzyme activity unit of the compound protease mixed with alkaline protease Alcalase and trypsin at a ratio of 2.8:1.5) is a compound protease in...

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PUM

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Abstract

The invention discloses a method for preparing peanut non-starch polysaccharides by utilizing hot pressed peanut meal. The method comprises the following steps: uniformly mixing peanut meal powder and distilled water, adjusting the pH value, adding compound plant hydrolase ViscozymeL, and carrying out microwave heating for enzymolysis to obtain enzymatic hydrolysate; adjusting the pH value of the enzymatic hydrolysate, adding compound protease, carrying out microwave heating for enzymolysis, killing enzyme and centrifuging; supernate is added to absolute ethyl alcohol with the volume of 4 times of that of the supernate, standing overnight in a refrigerator, centrifuging, precipitating, washing with ethyl alcohol and acetone, and freeze-drying to obtain the peanut non-starch polysaccharides. The peanut non-starch polysaccharides have functional activity of resisting oxidation and degreasing blood sugar and are suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing peanut non-starch polysaccharides by using hot-pressed peanut meal, and belongs to the field of food nutritional active substance processing. Background technique [0002] Peanut is an annual herbaceous plant of leguminous family, containing 25%-36% protein and 10%-23% carbohydrate. The light brown or dark brown small block or powdery by-products left after peanut oil extraction is called peanut meal, with an annual output of 4 million tons, which is a bulk protein and polysaccharide resource. Soluble non-starch polysaccharides can be obtained from the enzymatic hydrolyzate of peanut meal by ethanol precipitation. It is a kind of mixed polysaccharides including reducing sugars, neutral sugars, uronic acids or glycoproteins. Tumor, hypoglycemic, improve immunity and so on. Diabetes is a disorder of sugar, fat and protein metabolism. The cause is increased glucose production, but weakened utilization, res...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04
Inventor 齐宏涛
Owner 青岛博之源生物技术有限公司